LOCUS       BBa_K362012             1884 bp    DNA     linear       22-Nov-2024
DEFINITION  sufA promoter - RBS - RFP - Term - Term
ACCESSION   BBa_K362012
VERSION     BBa_K362012.1  
FEATURES             Location/Qualifiers
     promoter        1..1000
                     /label=BBa_K362001
     RBS             1009..1021
                     /label=RBS-3\Weak
     RBS             1015..1018
                     /label=RBS
     misc_feature    1009..1021
                     /label=BBa_B0032
     RBS             1009..1021
                     /label=BBa_B0032
     CDS             1028..1747
                     /label=GFP protein
     CDS             1028..1747
                     /label=BBa_E0040
     stem_loop       1767..1810
                     /label=stem_loop
     misc_feature    1756..1835
                     /label=BBa_B0010
     terminator      1756..1835
                     /label=BBa_B0010
     stem_loop       1851..1870
                     /label=T7 TE
     misc_feature    1877..1877
                     /label=stop
     polyA_site      1871..1884
                     /label=polya
     misc_feature    1844..1884
                     /label=BBa_B0012
     terminator      1844..1884
                     /label=BBa_B0012
     misc_feature    1009..1884
                     /label=BBa_E0240
ORIGIN
        1 tccatcagtt tctcattgtc acttaacact ttcatttgct ctgtacttac gtttgcaggc
       61 acgtttagct taagcttgcc accagaggct tcatattgtt gccaggtttt tgcgggatct
      121 ttggaggcaa gaattgccgc tgctgtcgcc gggctttctg gcttgataac cccaaccaac
      181 aatgttctta attgcacttt gccagaatct acccacgggc gcgcctgctg ccagaactgt
      241 ttacaatatg ggcagaacgg atcggcgaag acgtagacaa tgaccggcgc atctttttta
      301 ccgtcgagga gccagtggga ttgttccatc cgttgccaca tttcgcgtcc ggctggtgcg
      361 taaatttctt tttcgataag tgtgttactc aggttttcac ctttctcgtt gtacatgtaa
      421 ccagagatag cgtgcttacc atctggagtc aggtagatgg tgacgcccat atcctgatac
      481 tttccgagat aacctttcat tcctccgggg gcatcgaatg ttttgatgat tgtaatgccc
      541 tgtttttcaa tcgcttttac tggagcagga agttcctctg cgaaggcgat tgcaggaagc
      601 agagccagta aaagtatctt ttttaacatt aatttgtcct tttcagtcag tgcaaaagtc
      661 gagtaaaagg cataacctat cactgtcata ggtaagagct tagatcaggt gattgccctt
      721 tgtttatgag ggtgttgtaa tccatgtcgt tgttgcattt gtaagggcaa cacctcagcc
      781 tgcaggcagg cactgaagat accaaagggt agttcagatt acacggtcac ctggaaaggg
      841 ggccatttta ctttttatcg ccgctggcgg tgcaaagttc acaaagttgt cttacgaagg
      901 ttgtaaggta aaacttatcg atttgataat ggaaacgcat tagccgaatc ggcaaaaatt
      961 ggttacctta catctcatcg aaaacacgga ggaagtatag tactagagtc acacaggaaa
     1021 gtactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     1081 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     1141 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     1201 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
     1261 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     1321 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     1381 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     1441 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
     1501 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     1561 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1621 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     1681 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     1741 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1801 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1861 cgggtgggcc tttctgcgtt tata
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