LOCUS       BBa_K362014             1196 bp    DNA     linear       25-Nov-2024
DEFINITION  acrAB promoter - RBS - RFP - Term - Term
ACCESSION   BBa_K362014
VERSION     BBa_K362014.1  
FEATURES             Location/Qualifiers
     promoter        1..327
                     /label=BBa_K362000
     misc_feature    340..343
                     /label=conserved
     RBS             336..347
                     /label=BBa_B0034
     CDS             354..1028
                     /label=mrfp1
     misc_feature    1035..1059
                     /label=Help:Barcodes
     CDS             354..1034
                     /label=BBa_E1010
     stem_loop       1079..1122
                     /label=stem_loop
     misc_feature    1068..1147
                     /label=BBa_B0010
     terminator      1068..1147
                     /label=BBa_B0010
     stem_loop       1163..1182
                     /label=T7 TE
     misc_feature    1189..1189
                     /label=stop
     polyA_site      1183..1196
                     /label=polya
     misc_feature    1156..1196
                     /label=BBa_B0012
     terminator      1156..1196
                     /label=BBa_B0012
     misc_feature    336..1196
                     /label=BBa_I13507
ORIGIN
        1 gatctcactg aacaaatccg acttgtcttt aaaatgccac tagattgcac cgcgcgtaac
       61 gccagctgct ttttgcaatc tcgcccagcg aggtggatga taccccctgc tgtgagaaaa
      121 gacgtagagc cacatcgagg atgtgttggc gcgtttcttg cgcttcttgt ttggtttttc
      181 gtgccatatg ttcgtgaatt tacaggcgtt agatttacat acatttgtga atgtatgtac
      241 catagcacga cgataatata aacgcagcaa tgggtttatt aacttttgac cattgaccaa
      301 tttgaaatcg gacactcgag gtttacatac tagagaaaga ggagaaatac tagatggctt
      361 cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta
      421 acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga
      481 ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc
      541 cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc
      601 tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg
      661 gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta
      721 aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt
      781 gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa
      841 tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg
      901 ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct
      961 cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca
     1021 ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat
     1081 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1141 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//