LOCUS       BBa_K362016             1369 bp    DNA     linear       17-May-2024
DEFINITION  dps promoter - RBS - RFP - Term - Term
ACCESSION   BBa_K362016
VERSION     BBa_K362016.1  
FEATURES             Location/Qualifiers
     promoter        1..500
                     /label=BBa_K362002
     misc_feature    513..516
                     /label=conserved
     RBS             509..520
                     /label=BBa_B0034
     CDS             527..1201
                     /label=mrfp1
     misc_feature    1208..1232
                     /label=Help:Barcodes
     CDS             527..1207
                     /label=BBa_E1010
     stem_loop       1252..1295
                     /label=stem_loop
     misc_feature    1241..1320
                     /label=BBa_B0010
     terminator      1241..1320
                     /label=BBa_B0010
     stem_loop       1336..1355
                     /label=T7 TE
     misc_feature    1362..1362
                     /label=stop
     polyA_site      1356..1369
                     /label=polya
     misc_feature    1329..1369
                     /label=BBa_B0012
     terminator      1329..1369
                     /label=BBa_B0012
     misc_feature    509..1369
                     /label=BBa_I13507
ORIGIN
        1 cctcacccgt ttgccaacac ggacatttgg tacgctgatg agcatggaac cggcgctggc
       61 tgccgtttcc gggatgattt tcctcggaga aacactgaca cccatacagc tactggcgct
      121 cggcgctatc atcgccgctt caatggggtc tacgctgaca gtacgcaaag agagcaaaat
      181 aaaagaatta gacattaatt aaatttacat ttctgcatgg ttatgcataa ccatgcagaa
      241 tttctcgcta cttttcctct acaccgtctt tatatatcga attatgcaaa agcatattta
      301 ttccgaaaat tcctggcgag cagataaata agaattgttc ttatcaatat atctaactca
      361 ttgaatcttt attagttttg tttttcacgc ttgttaccac tattagtgtg ataggaacag
      421 ccagaatagc ggaacacata gccggtgcta tacttaatct cgttaattac tgggacataa
      481 catcaagagg atatgaaatt tactagagaa agaggagaaa tactagatgg cttcctccga
      541 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      601 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      661 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      721 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      781 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      841 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      901 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      961 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
     1021 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
     1081 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
     1141 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
     1201 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
     1261 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1321 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//