LOCUS       BBa_K362018             1869 bp    DNA     linear       29-Nov-2024
DEFINITION  sodA promoter - RBS - RFP - Term - Term
ACCESSION   BBa_K362018
VERSION     BBa_K362018.1  
FEATURES             Location/Qualifiers
     promoter        1..1000
                     /label=BBa_K362004
     misc_feature    1013..1016
                     /label=conserved
     RBS             1009..1020
                     /label=BBa_B0034
     CDS             1027..1701
                     /label=mrfp1
     misc_feature    1708..1732
                     /label=Help:Barcodes
     CDS             1027..1707
                     /label=BBa_E1010
     stem_loop       1752..1795
                     /label=stem_loop
     misc_feature    1741..1820
                     /label=BBa_B0010
     terminator      1741..1820
                     /label=BBa_B0010
     stem_loop       1836..1855
                     /label=T7 TE
     misc_feature    1862..1862
                     /label=stop
     polyA_site      1856..1869
                     /label=polya
     misc_feature    1829..1869
                     /label=BBa_B0012
     terminator      1829..1869
                     /label=BBa_B0012
     misc_feature    1009..1869
                     /label=BBa_I13507
ORIGIN
        1 acttttgcca gacgaataaa acagaaaccg aggttaatga tcgcgccgcc gcccatgatg
       61 acaacatagc ttggcagagc gacatacagt ggatcgacgc caagtgcggc agcggcttca
      121 tgcatcggtt ttgcggcgtt catcgcaaag gacatcccgg cagagaaaat gccgcacatc
      181 accgccagca ccagcccttt tttcagattg aactcttcgg ctttaatgcc catcttgcgc
      241 tctttcaact gcccggcgcg agttacaatc cctacgccaa tcagcgccac cagaacgccg
      301 agcaacgtca tgcgtccgcc ttcggtgcta atcaacacat cgaaattgcc gttgataatt
      361 ggcgtcatca gcgtaccgac aatcaacgta atgccaatgg cgatgccaat tcccatcgac
      421 atgccgagat aacgcatggt caggccgtag ttgatattac cgatccccca catagcgccg
      481 aacagaaaaa caggcagtcg cgtagagaga ctaaacgagc tgtaatacgc ccagaaattc
      541 ggtagtaaca gggcgctgat ggcccacggc agaataatcc acgaaacaat cccaccgact
      601 gaccacatgg tttcccatga ccattttttt acttttttga acggagcgta aaaacaggct
      661 gcactggccg cgccgatcaa atgccaaaat atccccatcg taatcgcgtt actcatcttc
      721 ttatcctcat catttttcgt cgcgtcacat ctccgacgag atgagtgtaa aaatcgtgct
      781 gtcgattaac ctttcgcctg ttgccgccgt tgtcgattta ctggcaatca cggcattaag
      841 tgggtgattt gcttcacatc tcgggcattt tcctgcaaaa ccataccctt acgaaaagta
      901 cggcattgat aatcattttc aatatcattt aattaactat aatgaaccaa ctgcttacgc
      961 ggcattaaca atcggccgcc cgacaatact ggagatgaat tactagagaa agaggagaaa
     1021 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg
     1081 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac
     1141 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg
     1201 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac
     1261 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac
     1321 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc
     1381 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
     1441 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
     1501 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
     1561 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
     1621 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
     1681 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag
     1741 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1801 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1861 gcgtttata
//