LOCUS BBa_K362051 3816 bp DNA linear 22-Dec-2024 DEFINITION ahpC promoter - RBS - GFP - Term - Term - sufA promoter - RBS - RFP - Term - Term ACCESSION BBa_K362051 VERSION BBa_K362051.1 FEATURES Location/Qualifiers promoter 1..1000 /label=BBa_K362001 misc_feature 1009..1028 /label=BBa_J54033 misc_feature 1009..1014 /label=SalI misc_feature 1023..1028 /label=BamHI misc_feature 1035..1054 /label=BBa_J54025 misc_feature 1035..1040 /label=BglII misc_feature 1049..1054 /label=MulI RBS 1057..1072 /label=RBS-1\Strong misc_feature 1057..1072 /label=BBa_B0030 RBS 1064..1070 /label=RBS misc_feature 1079..1798 /label=BBa_E0040 misc_feature 1808..1887 /label=BBa_B0010 misc_feature 1895..1935 /label=BBa_B0012 stem_loop 1819..1862 /label=stem_loop stem_loop 1907..1926 /label=T7 TE misc_feature 1009..1939 /label=BBa_J54103 stem_loop 1823..1866 /label=stem_loop misc_feature 1812..1891 /label=BBa_B0010 terminator 1812..1891 /label=BBa_B0010 stem_loop 1907..1926 /label=T7 TE misc_feature 1933..1933 /label=stop polyA_site 1927..1940 /label=polya misc_feature 1900..1940 /label=BBa_B0012 terminator 1900..1939 /label=BBa_B0012 misc_feature 1009..1013 /label=SalI misc_feature 1009..1027 /label=BBa_J54033 misc_feature 1023..1027 /label=BamHI misc_feature 1036..1040 /label=BglII misc_feature 1036..1054 /label=BBa_J54025 misc_feature 1049..1054 /label=MulI RBS 1063..1077 /label=RBS-1\Strong misc_feature 1063..1077 /label=BBa_B0030 RBS 1070..1074 /label=RBS misc_feature 1084..1803 /label=BBa_E0040 misc_feature 1812..1891 /label=BBa_B0010 stem_loop 1823..1866 /label=stem_loop misc_feature 1900..1940 /label=BBa_B0012 stem_loop 1907..1926 /label=T7 TE polyA_site 1927..1940 /label=polya misc_feature 1933..1933 /label=stop misc_feature 1009..1939 /label=BBa_J54103 misc_feature 1009..1939 /label=BBa_K121013 promoter 1948..2947 /label=BBa_K362005 misc_feature 2960..2963 /label=conserved RBS 2956..2967 /label=BBa_B0034 CDS 2974..3648 /label=mrfp1 misc_feature 3655..3679 /label=Help:Barcodes CDS 2974..3654 /label=BBa_E1010 stem_loop 3699..3742 /label=stem_loop misc_feature 3688..3767 /label=BBa_B0010 terminator 3688..3767 /label=BBa_B0010 stem_loop 3783..3802 /label=T7 TE misc_feature 3809..3809 /label=stop polyA_site 3803..3816 /label=polya misc_feature 3776..3816 /label=BBa_B0012 terminator 3776..3816 /label=BBa_B0012 misc_feature 2956..3816 /label=BBa_I13507 ORIGIN 1 tccatcagtt tctcattgtc acttaacact ttcatttgct ctgtacttac gtttgcaggc 61 acgtttagct taagcttgcc accagaggct tcatattgtt gccaggtttt tgcgggatct 121 ttggaggcaa gaattgccgc tgctgtcgcc gggctttctg gcttgataac cccaaccaac 181 aatgttctta attgcacttt gccagaatct acccacgggc gcgcctgctg ccagaactgt 241 ttacaatatg ggcagaacgg atcggcgaag acgtagacaa tgaccggcgc atctttttta 301 ccgtcgagga gccagtggga ttgttccatc cgttgccaca tttcgcgtcc ggctggtgcg 361 taaatttctt tttcgataag tgtgttactc aggttttcac ctttctcgtt gtacatgtaa 421 ccagagatag cgtgcttacc atctggagtc aggtagatgg tgacgcccat atcctgatac 481 tttccgagat aacctttcat tcctccgggg gcatcgaatg ttttgatgat tgtaatgccc 541 tgtttttcaa tcgcttttac tggagcagga agttcctctg cgaaggcgat tgcaggaagc 601 agagccagta aaagtatctt ttttaacatt aatttgtcct tttcagtcag tgcaaaagtc 661 gagtaaaagg cataacctat cactgtcata ggtaagagct tagatcaggt gattgccctt 721 tgtttatgag ggtgttgtaa tccatgtcgt tgttgcattt gtaagggcaa cacctcagcc 781 tgcaggcagg cactgaagat accaaagggt agttcagatt acacggtcac ctggaaaggg 841 ggccatttta ctttttatcg ccgctggcgg tgcaaagttc acaaagttgt cttacgaagg 901 ttgtaaggta aaacttatcg atttgataat ggaaacgcat tagccgaatc ggcaaaaatt 961 ggttacctta catctcatcg aaaacacgga ggaagtatag tactagaggt cgactgacga 1021 ctggatccta tcaagatctg tcggataacg cgttactaga gattaaagag gagaaatact 1081 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg 1141 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg 1201 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac 1261 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac 1321 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt 1381 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg 1441 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca 1501 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg 1561 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag 1621 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt 1681 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc 1741 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat 1801 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 1861 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 1921 gggcctttct gcgtttatat actagagctg gaaatcaatg ctaaccacgt ccgctcggca 1981 cgagaagggc gggtgcgcgg cgtatgcaaa ccgttgcatc tcggttcgcg tcaccaggtc 2041 tggcagattg aaatcttcga tgagaaaggg cgtttgtgct gttcgtcacg attgacgacc 2101 gccattttgt gacgttgtca ccagaaaagt gtgacgcata ttgcttttgg tcattatctg 2161 cacagtaaac ggtggtatac tgtgcaggtc ttgtctaaaa gcgaggatgc tatgtctact 2221 caacttgatc ccacccagct ggccattgaa tttttacgcc gtgaccagag caatctttca 2281 cctgcccaat atctcaaacg tctgaaacaa ctagaacttg aatttgctga cctgcttact 2341 ctctcttctg ctgaattaaa agaagaaatc tactttgcct ggcgtctggg cgtgcattga 2401 tcctgactta atgccgcaag ttcgccaaat cgacctggat taacctccgt ccaggttcga 2461 tgaccttctt aatactgctt atctaccgcc tgatattgtt caaaaataat actaatcgca 2521 gtttgtgtta aaacggcggg ttagctttat gagattattc ttatcgcccc ttcaagagct 2581 aagccactga gagtgccgga gataagcgcc ggatggggta gaaaccctta agcctgtgtc 2641 gcacagactt aagggttttc ttatttctga acgctgcact tttttaccta tctcttaaat 2701 agtcattttc aatacaactt tttctttttc atcacagcct taatgctcct tagataataa 2761 ccattatcta acaatgagat acctaattct tagcgtgcct gttaacccac acatcagggt 2821 ctatgcttat taaaagcagg caaaaagcat gttttacggt aaagcccctg cgtttgctgg 2881 gttgaactga taatcattat cactaacatg ctgttatacg ctgaaagcga tgaagtgagg 2941 taaatcgtac tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa 3001 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc 3061 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc 3121 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc 3181 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa 3241 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag 3301 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc 3361 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt 3421 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac 3481 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg 3541 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc 3601 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga 3661 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga 3721 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 3781 ctggctcacc ttcgggtggg cctttctgcg tttata //