LOCUS BBa_K362052 3816 bp DNA linear 22-Nov-2024 DEFINITION ahpC promoter - RBS - RFP - Term - Term - sufA promoter - RBS - GFP - Term - Term ACCESSION BBa_K362052 VERSION BBa_K362052.1 FEATURES Location/Qualifiers promoter 1..1000 /label=BBa_K362001 misc_feature 1013..1016 /label=conserved RBS 1009..1020 /label=BBa_B0034 CDS 1027..1701 /label=mrfp1 misc_feature 1708..1732 /label=Help:Barcodes CDS 1027..1707 /label=BBa_E1010 stem_loop 1752..1795 /label=stem_loop misc_feature 1741..1820 /label=BBa_B0010 terminator 1741..1820 /label=BBa_B0010 stem_loop 1836..1855 /label=T7 TE misc_feature 1862..1862 /label=stop polyA_site 1856..1869 /label=polya misc_feature 1829..1869 /label=BBa_B0012 terminator 1829..1869 /label=BBa_B0012 misc_feature 1009..1869 /label=BBa_I13507 promoter 1878..2877 /label=BBa_K362005 misc_feature 2886..2905 /label=BBa_J54033 misc_feature 2886..2891 /label=SalI misc_feature 2900..2905 /label=BamHI misc_feature 2912..2931 /label=BBa_J54025 misc_feature 2912..2917 /label=BglII misc_feature 2926..2931 /label=MulI RBS 2934..2949 /label=RBS-1\Strong misc_feature 2934..2949 /label=BBa_B0030 RBS 2941..2947 /label=RBS misc_feature 2956..3675 /label=BBa_E0040 misc_feature 3685..3764 /label=BBa_B0010 misc_feature 3772..3812 /label=BBa_B0012 stem_loop 3696..3739 /label=stem_loop stem_loop 3784..3803 /label=T7 TE misc_feature 2886..3816 /label=BBa_J54103 stem_loop 3700..3743 /label=stem_loop misc_feature 3689..3768 /label=BBa_B0010 terminator 3689..3768 /label=BBa_B0010 stem_loop 3784..3803 /label=T7 TE misc_feature 3810..3810 /label=stop polyA_site 3804..3817 /label=polya misc_feature 3777..3817 /label=BBa_B0012 terminator 3777..3816 /label=BBa_B0012 misc_feature 2886..2890 /label=SalI misc_feature 2886..2904 /label=BBa_J54033 misc_feature 2900..2904 /label=BamHI misc_feature 2913..2917 /label=BglII misc_feature 2913..2931 /label=BBa_J54025 misc_feature 2926..2931 /label=MulI RBS 2940..2954 /label=RBS-1\Strong misc_feature 2940..2954 /label=BBa_B0030 RBS 2947..2951 /label=RBS misc_feature 2961..3680 /label=BBa_E0040 misc_feature 3689..3768 /label=BBa_B0010 stem_loop 3700..3743 /label=stem_loop misc_feature 3777..3817 /label=BBa_B0012 stem_loop 3784..3803 /label=T7 TE polyA_site 3804..3817 /label=polya misc_feature 3810..3810 /label=stop misc_feature 2886..3816 /label=BBa_J54103 misc_feature 2886..3816 /label=BBa_K121013 ORIGIN 1 tccatcagtt tctcattgtc acttaacact ttcatttgct ctgtacttac gtttgcaggc 61 acgtttagct taagcttgcc accagaggct tcatattgtt gccaggtttt tgcgggatct 121 ttggaggcaa gaattgccgc tgctgtcgcc gggctttctg gcttgataac cccaaccaac 181 aatgttctta attgcacttt gccagaatct acccacgggc gcgcctgctg ccagaactgt 241 ttacaatatg ggcagaacgg atcggcgaag acgtagacaa tgaccggcgc atctttttta 301 ccgtcgagga gccagtggga ttgttccatc cgttgccaca tttcgcgtcc ggctggtgcg 361 taaatttctt tttcgataag tgtgttactc aggttttcac ctttctcgtt gtacatgtaa 421 ccagagatag cgtgcttacc atctggagtc aggtagatgg tgacgcccat atcctgatac 481 tttccgagat aacctttcat tcctccgggg gcatcgaatg ttttgatgat tgtaatgccc 541 tgtttttcaa tcgcttttac tggagcagga agttcctctg cgaaggcgat tgcaggaagc 601 agagccagta aaagtatctt ttttaacatt aatttgtcct tttcagtcag tgcaaaagtc 661 gagtaaaagg cataacctat cactgtcata ggtaagagct tagatcaggt gattgccctt 721 tgtttatgag ggtgttgtaa tccatgtcgt tgttgcattt gtaagggcaa cacctcagcc 781 tgcaggcagg cactgaagat accaaagggt agttcagatt acacggtcac ctggaaaggg 841 ggccatttta ctttttatcg ccgctggcgg tgcaaagttc acaaagttgt cttacgaagg 901 ttgtaaggta aaacttatcg atttgataat ggaaacgcat tagccgaatc ggcaaaaatt 961 ggttacctta catctcatcg aaaacacgga ggaagtatag tactagagaa agaggagaaa 1021 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg 1081 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac 1141 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg 1201 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac 1261 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac 1321 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc 1381 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa 1441 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa 1501 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa 1561 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa 1621 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa 1681 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag 1741 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1801 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1861 gcgtttatat actagagctg gaaatcaatg ctaaccacgt ccgctcggca cgagaagggc 1921 gggtgcgcgg cgtatgcaaa ccgttgcatc tcggttcgcg tcaccaggtc tggcagattg 1981 aaatcttcga tgagaaaggg cgtttgtgct gttcgtcacg attgacgacc gccattttgt 2041 gacgttgtca ccagaaaagt gtgacgcata ttgcttttgg tcattatctg cacagtaaac 2101 ggtggtatac tgtgcaggtc ttgtctaaaa gcgaggatgc tatgtctact caacttgatc 2161 ccacccagct ggccattgaa tttttacgcc gtgaccagag caatctttca cctgcccaat 2221 atctcaaacg tctgaaacaa ctagaacttg aatttgctga cctgcttact ctctcttctg 2281 ctgaattaaa agaagaaatc tactttgcct ggcgtctggg cgtgcattga tcctgactta 2341 atgccgcaag ttcgccaaat cgacctggat taacctccgt ccaggttcga tgaccttctt 2401 aatactgctt atctaccgcc tgatattgtt caaaaataat actaatcgca gtttgtgtta 2461 aaacggcggg ttagctttat gagattattc ttatcgcccc ttcaagagct aagccactga 2521 gagtgccgga gataagcgcc ggatggggta gaaaccctta agcctgtgtc gcacagactt 2581 aagggttttc ttatttctga acgctgcact tttttaccta tctcttaaat agtcattttc 2641 aatacaactt tttctttttc atcacagcct taatgctcct tagataataa ccattatcta 2701 acaatgagat acctaattct tagcgtgcct gttaacccac acatcagggt ctatgcttat 2761 taaaagcagg caaaaagcat gttttacggt aaagcccctg cgtttgctgg gttgaactga 2821 taatcattat cactaacatg ctgttatacg ctgaaagcga tgaagtgagg taaatcgtac 2881 tagaggtcga ctgacgactg gatcctatca agatctgtcg gataacgcgt tactagagat 2941 taaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat 3001 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga 3061 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc 3121 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata 3181 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca 3241 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt 3301 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg 3361 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc 3421 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg 3481 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct 3541 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa 3601 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga 3661 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 3721 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 3781 ctggctcacc ttcgggtggg cctttctgcg tttata //