BBa_K362001 1 BBa_K362001 ahpC, this promoter is controlled by the OxyR protein. 2010-09-21T11:00:00Z 2015-05-08T01:12:12Z This promoter , Pahpc , exists on the upper stream&#12288;of ahpC. This promoter has sensitivity to oxidative stress. false false _480_ 0 6267 9 It's complicated true TCCATCAGTTTCTCATTGTCACTTAACACTTTCATTTGCTCTGTACTTACGTTTGCAGGCACGTTTAGCTTAAGCTTGCCACCAGAGGCTTCATATTGTTGCCAGGTTTTTGCGGGATCTTTGGAGGCAAGAATTGCCGCTGCTGTCGCCGGGCTTTCTGGCTTGATAACCCCAACCAACAATGTTCTTAATTGCACTTTGCCAGAATCTACCCACGGGCGCGCCTGCTGCCAGAACTGTTTACAATATGGGCAGAACGGATCGGCGAAGACGTAGACAATGACCGGCGCATCTTTTTTACCGTCGAGGAGCCAGTGGGATTGTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCAGATTACACGGTCACCTGGAAAGGGGGCCATTTTACTTTTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTACATCTCATCGAAAACACGGAGGAAGTATAG false Fumihiko Takeuchi,Aya Iwaki,Yuki Fukuyama,Satori Furumiti, Kazuma Usui, Akiko Kawashima, Toyokazu Nakamura,Reo Yoshimura BBa_K121013 1 BBa_K121013 Promoterless GFP on pSB6 2008-10-15T11:00:00Z 2015-05-08T01:09:41Z na Promoterless GFP reporter false false _220_ 0 3180 9 It's complicated false na false Masashi Kajita annotation1981714 1 T7 TE range1981714 1 899 918 annotation1981707 1 RBS-1\Strong range1981707 1 55 69 annotation1981701 1 SalI range1981701 1 1 5 annotation1981709 1 RBS range1981709 1 62 66 annotation1981713 1 BBa_B0012 range1981713 1 892 932 annotation1981711 1 BBa_B0010 range1981711 1 804 883 annotation1981712 1 stem_loop range1981712 1 815 858 annotation1981706 1 MulI range1981706 1 41 46 annotation1981716 1 stop range1981716 1 925 925 annotation1981703 1 BamHI range1981703 1 15 19 annotation1981708 1 BBa_B0030 range1981708 1 55 69 annotation1981704 1 BglII range1981704 1 28 32 annotation1981705 1 BBa_J54025 range1981705 1 28 46 annotation1981715 1 polya range1981715 1 919 932 annotation1981702 1 BBa_J54033 range1981702 1 1 19 annotation1981710 1 BBa_E0040 range1981710 1 76 795 annotation1981717 1 BBa_J54103 range1981717 1 1 931 BBa_J54103 1 BBa_J54103 Promoterless-GFP(A) 2006-10-16T11:00:00Z 2015-08-31T03:48:11Z synthesis Released HQ 2013 promoterless false true _76_ 0 762 76 In stock true as negative control true Yusaku Nakashima annotation2152490 1 SalI range2152490 1 1 6 annotation2152491 1 BamHI range2152491 1 15 20 annotation2152489 1 BBa_J54033 range2152489 1 1 20 annotation2152499 1 BBa_B0010 range2152499 1 800 879 annotation2152502 1 T7 TE range2152502 1 899 918 annotation2152493 1 BglII range2152493 1 27 32 annotation2152500 1 BBa_B0012 range2152500 1 887 927 annotation2152494 1 MulI range2152494 1 41 46 annotation2152496 1 BBa_B0030 range2152496 1 49 64 annotation2152492 1 BBa_J54025 range2152492 1 27 46 annotation2152495 1 RBS-1\Strong range2152495 1 49 64 annotation2152497 1 RBS range2152497 1 56 62 annotation2152501 1 stem_loop range2152501 1 811 854 annotation2152498 1 BBa_E0040 range2152498 1 71 790 BBa_I13507 1 BBa_I13507 Screening plasmid intermediate 2005-05-30T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 Built by Josh as an intermediate in screening plasmid construction. false false _11_ 0 253 6 In stock false true jkm component1524838 1 BBa_B0012 component1524815 1 BBa_B0034 component1524828 1 BBa_B0010 component1524822 1 BBa_E1010 annotation1524822 1 BBa_E1010 range1524822 1 19 699 annotation1524828 1 BBa_B0010 range1524828 1 733 812 annotation1524838 1 BBa_B0012 range1524838 1 821 861 annotation1524815 1 BBa_B0034 range1524815 1 1 12 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K362005 1 BBa_K362005 sufA , this promoter is controlled by OxyR. 2010-09-24T11:00:00Z 2015-05-08T01:12:12Z sufA , this promoter has sensitivity to oxidative stress. sufA , this promoter has sensitivity to oxidative stress. false false _480_ 0 6267 9 Not in stock true sufA , this promoter has sensitivity to oxidative stress. false Fumihiko Takeuchi,Aya Iwaki,Yuki Fukuyama,Satori Furumiti, Kazuma Usui, Akiko Kawashima, Toyokazu Nakamura,Reo Yoshimura BBa_K362052 1 BBa_K362052 ahpC promoter - RBS - RFP - Term - Term - sufA promoter - RBS - GFP - Term - Term 2010-10-24T11:00:00Z 2015-05-08T01:12:13Z sufA Promoter from E.coli genome This composite part contains ahpC promoter(BBa_K362001),RBS(BBa_K121013),GFP(BBa_K121013),2 terminators(BBa_K121013),sufA promoter(BBa_K362005),RBS(BBa_I13507),RFP(BBa_I13507) and 2 terminators(BBa_I13507). sufA promoter has sensitibity to oxydative stress. ahpC promoter is controlled by OxyR protein, an oxidative stress resoponsive transcription facor. sufA promoter has sensitivity to oxidative stress. false false _480_ 0 7848 9 Not in stock false This composite part contains ahpC promoter(BBa_K362001),RBS(BBa_K121013),GFP(BBa_K121013),2 terminators(BBa_K121013),sufA promoter(BBa_K362005),RBS(BBa_I13507),RFP(BBa_I13507) and 2 terminators(BBa_I13507). sufA promoter has sensitibity to oxydative stress. ahpC promoter is controlled by OxyR protein, an oxidative stress resoponsive transcription facor. sufA promoter has sensitivity to oxidative stress. false Fumihiko Takeuchi, Aya Iwaki, Akiko Kawashima , Yuki Fukuyama component2102086 1 BBa_B0010 component2102076 1 BBa_J54103 component2102074 1 BBa_K362005 component2102088 1 BBa_B0012 component2102073 1 BBa_I13507 component2102092 1 BBa_K121013 component2102061 1 BBa_K362001 annotation2102092 1 BBa_K121013 range2102092 1 2886 3816 annotation2102074 1 BBa_K362005 range2102074 1 1878 2877 annotation2102086 1 BBa_B0010 range2102086 1 3689 3768 annotation2102088 1 BBa_B0012 range2102088 1 3777 3816 annotation2102061 1 BBa_K362001 range2102061 1 1 1000 annotation2102073 1 BBa_I13507 range2102073 1 1009 1869 annotation2102076 1 BBa_J54103 range2102076 1 2886 3816 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K362001_sequence 1 tccatcagtttctcattgtcacttaacactttcatttgctctgtacttacgtttgcaggcacgtttagcttaagcttgccaccagaggcttcatattgttgccaggtttttgcgggatctttggaggcaagaattgccgctgctgtcgccgggctttctggcttgataaccccaaccaacaatgttcttaattgcactttgccagaatctacccacgggcgcgcctgctgccagaactgtttacaatatgggcagaacggatcggcgaagacgtagacaatgaccggcgcatcttttttaccgtcgaggagccagtgggattgttccatccgttgccacatttcgcgtccggctggtgcgtaaatttctttttcgataagtgtgttactcaggttttcacctttctcgttgtacatgtaaccagagatagcgtgcttaccatctggagtcaggtagatggtgacgcccatatcctgatactttccgagataacctttcattcctccgggggcatcgaatgttttgatgattgtaatgccctgtttttcaatcgcttttactggagcaggaagttcctctgcgaaggcgattgcaggaagcagagccagtaaaagtatcttttttaacattaatttgtccttttcagtcagtgcaaaagtcgagtaaaaggcataacctatcactgtcataggtaagagcttagatcaggtgattgccctttgtttatgagggtgttgtaatccatgtcgttgttgcatttgtaagggcaacacctcagcctgcaggcaggcactgaagataccaaagggtagttcagattacacggtcacctggaaagggggccattttactttttatcgccgctggcggtgcaaagttcacaaagttgtcttacgaaggttgtaaggtaaaacttatcgatttgataatggaaacgcattagccgaatcggcaaaaattggttaccttacatctcatcgaaaacacggaggaagtatag BBa_K362052_sequence 1 tccatcagtttctcattgtcacttaacactttcatttgctctgtacttacgtttgcaggcacgtttagcttaagcttgccaccagaggcttcatattgttgccaggtttttgcgggatctttggaggcaagaattgccgctgctgtcgccgggctttctggcttgataaccccaaccaacaatgttcttaattgcactttgccagaatctacccacgggcgcgcctgctgccagaactgtttacaatatgggcagaacggatcggcgaagacgtagacaatgaccggcgcatcttttttaccgtcgaggagccagtgggattgttccatccgttgccacatttcgcgtccggctggtgcgtaaatttctttttcgataagtgtgttactcaggttttcacctttctcgttgtacatgtaaccagagatagcgtgcttaccatctggagtcaggtagatggtgacgcccatatcctgatactttccgagataacctttcattcctccgggggcatcgaatgttttgatgattgtaatgccctgtttttcaatcgcttttactggagcaggaagttcctctgcgaaggcgattgcaggaagcagagccagtaaaagtatcttttttaacattaatttgtccttttcagtcagtgcaaaagtcgagtaaaaggcataacctatcactgtcataggtaagagcttagatcaggtgattgccctttgtttatgagggtgttgtaatccatgtcgttgttgcatttgtaagggcaacacctcagcctgcaggcaggcactgaagataccaaagggtagttcagattacacggtcacctggaaagggggccattttactttttatcgccgctggcggtgcaaagttcacaaagttgtcttacgaaggttgtaaggtaaaacttatcgatttgataatggaaacgcattagccgaatcggcaaaaattggttaccttacatctcatcgaaaacacggaggaagtatagtactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctggaaatcaatgctaaccacgtccgctcggcacgagaagggcgggtgcgcggcgtatgcaaaccgttgcatctcggttcgcgtcaccaggtctggcagattgaaatcttcgatgagaaagggcgtttgtgctgttcgtcacgattgacgaccgccattttgtgacgttgtcaccagaaaagtgtgacgcatattgcttttggtcattatctgcacagtaaacggtggtatactgtgcaggtcttgtctaaaagcgaggatgctatgtctactcaacttgatcccacccagctggccattgaatttttacgccgtgaccagagcaatctttcacctgcccaatatctcaaacgtctgaaacaactagaacttgaatttgctgacctgcttactctctcttctgctgaattaaaagaagaaatctactttgcctggcgtctgggcgtgcattgatcctgacttaatgccgcaagttcgccaaatcgacctggattaacctccgtccaggttcgatgaccttcttaatactgcttatctaccgcctgatattgttcaaaaataatactaatcgcagtttgtgttaaaacggcgggttagctttatgagattattcttatcgccccttcaagagctaagccactgagagtgccggagataagcgccggatggggtagaaacccttaagcctgtgtcgcacagacttaagggttttcttatttctgaacgctgcacttttttacctatctcttaaatagtcattttcaatacaactttttctttttcatcacagccttaatgctccttagataataaccattatctaacaatgagatacctaattcttagcgtgcctgttaacccacacatcagggtctatgcttattaaaagcaggcaaaaagcatgttttacggtaaagcccctgcgtttgctgggttgaactgataatcattatcactaacatgctgttatacgctgaaagcgatgaagtgaggtaaatcgtactagaggtcgactgacgactggatcctatcaagatctgtcggataacgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_I13507_sequence 1 aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K121013_sequence 1 gtcgactgacgactggatcctatcaagatctgtcggataacgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J54103_sequence 1 gtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K362005_sequence 1 ctggaaatcaatgctaaccacgtccgctcggcacgagaagggcgggtgcgcggcgtatgcaaaccgttgcatctcggttcgcgtcaccaggtctggcagattgaaatcttcgatgagaaagggcgtttgtgctgttcgtcacgattgacgaccgccattttgtgacgttgtcaccagaaaagtgtgacgcatattgcttttggtcattatctgcacagtaaacggtggtatactgtgcaggtcttgtctaaaagcgaggatgctatgtctactcaacttgatcccacccagctggccattgaatttttacgccgtgaccagagcaatctttcacctgcccaatatctcaaacgtctgaaacaactagaacttgaatttgctgacctgcttactctctcttctgctgaattaaaagaagaaatctactttgcctggcgtctgggcgtgcattgatcctgacttaatgccgcaagttcgccaaatcgacctggattaacctccgtccaggttcgatgaccttcttaatactgcttatctaccgcctgatattgttcaaaaataatactaatcgcagtttgtgttaaaacggcgggttagctttatgagattattcttatcgccccttcaagagctaagccactgagagtgccggagataagcgccggatggggtagaaacccttaagcctgtgtcgcacagacttaagggttttcttatttctgaacgctgcacttttttacctatctcttaaatagtcattttcaatacaactttttctttttcatcacagccttaatgctccttagataataaccattatctaacaatgagatacctaattcttagcgtgcctgttaacccacacatcagggtctatgcttattaaaagcaggcaaaaagcatgttttacggtaaagcccctgcgtttgctgggttgaactgataatcattatcactaacatgctgttatacgctgaaagcgatgaagtgaggtaaatcg BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z