##gff-version 3
##sequence-region BBa_K362052 1 3816
BBa_K362052	.	engineered_region	2886	3816	.	+	0	ID=BBa_J54103;Name=BBa_J54103
BBa_K362052	.	tag	2926	2931	.	+	0	ID=annotation2152494;Name=MulI;Parent=BBa_J54103
BBa_K362052	.	engineered_region	3772	3812	.	+	0	ID=annotation2152500;Name=BBa_B0012;Parent=BBa_J54103
BBa_K362052	.	engineered_region	2912	2931	.	+	0	ID=annotation2152492;Name=BBa_J54025;Parent=BBa_J54103
BBa_K362052	.	ribosome_entry_site	2934	2949	.	+	0	ID=annotation2152495;Name=RBS-1\Strong;Parent=BBa_J54103
BBa_K362052	.	engineered_region	2934	2949	.	+	0	ID=annotation2152496;Name=BBa_B0030;Parent=BBa_J54103
BBa_K362052	.	engineered_region	3685	3764	.	+	0	ID=annotation2152499;Name=BBa_B0010;Parent=BBa_J54103
BBa_K362052	.	stem_loop	3696	3739	.	+	0	ID=annotation2152501;Name=stem_loop;Parent=BBa_J54103
BBa_K362052	.	engineered_region	2886	2905	.	+	0	ID=annotation2152489;Name=BBa_J54033;Parent=BBa_J54103
BBa_K362052	.	engineered_region	2956	3675	.	+	0	ID=annotation2152498;Name=BBa_E0040;Parent=BBa_J54103
BBa_K362052	.	tag	2900	2905	.	+	0	ID=annotation2152491;Name=BamHI;Parent=BBa_J54103
BBa_K362052	.	tag	2886	2891	.	+	0	ID=annotation2152490;Name=SalI;Parent=BBa_J54103
BBa_K362052	.	stem_loop	3784	3803	.	+	0	ID=annotation2152502;Name=T7 TE;Parent=BBa_J54103
BBa_K362052	.	tag	2912	2917	.	+	0	ID=annotation2152493;Name=BglII;Parent=BBa_J54103
BBa_K362052	.	ribosome_entry_site	2941	2947	.	+	0	ID=annotation2152497;Name=RBS;Parent=BBa_J54103
BBa_K362052	.	engineered_region	2886	3816	.	+	0	ID=BBa_K121013;Name=BBa_K121013
BBa_K362052	.	stop_codon	3810	3810	.	+	0	ID=annotation1981716;Name=stop;Parent=BBa_K121013
BBa_K362052	.	engineered_region	2886	3816	.	+	0	ID=annotation1981717;Name=BBa_J54103;Parent=BBa_K121013
BBa_K362052	.	engineered_region	3777	3817	.	+	0	ID=annotation1981713;Name=BBa_B0012;Parent=BBa_K121013
BBa_K362052	.	tag	2926	2931	.	+	0	ID=annotation1981706;Name=MulI;Parent=BBa_K121013
BBa_K362052	.	engineered_region	2940	2954	.	+	0	ID=annotation1981708;Name=BBa_B0030;Parent=BBa_K121013
BBa_K362052	.	engineered_region	3689	3768	.	+	0	ID=annotation1981711;Name=BBa_B0010;Parent=BBa_K121013
BBa_K362052	.	engineered_region	2913	2931	.	+	0	ID=annotation1981705;Name=BBa_J54025;Parent=BBa_K121013
BBa_K362052	.	tag	2900	2904	.	+	0	ID=annotation1981703;Name=BamHI;Parent=BBa_K121013
BBa_K362052	.	polyA_site	3804	3817	.	+	0	ID=annotation1981715;Name=polya;Parent=BBa_K121013
BBa_K362052	.	stem_loop	3700	3743	.	+	0	ID=annotation1981712;Name=stem_loop;Parent=BBa_K121013
BBa_K362052	.	ribosome_entry_site	2940	2954	.	+	0	ID=annotation1981707;Name=RBS-1\Strong;Parent=BBa_K121013
BBa_K362052	.	stem_loop	3784	3803	.	+	0	ID=annotation1981714;Name=T7 TE;Parent=BBa_K121013
BBa_K362052	.	tag	2913	2917	.	+	0	ID=annotation1981704;Name=BglII;Parent=BBa_K121013
BBa_K362052	.	ribosome_entry_site	2947	2951	.	+	0	ID=annotation1981709;Name=RBS;Parent=BBa_K121013
BBa_K362052	.	tag	2886	2890	.	+	0	ID=annotation1981701;Name=SalI;Parent=BBa_K121013
BBa_K362052	.	engineered_region	2886	2904	.	+	0	ID=annotation1981702;Name=BBa_J54033;Parent=BBa_K121013
BBa_K362052	.	engineered_region	2961	3680	.	+	0	ID=annotation1981710;Name=BBa_E0040;Parent=BBa_K121013
BBa_K362052	.	terminator	3689	3768	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K362052	.	engineered_region	3689	3768	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K362052	.	stem_loop	3700	3743	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K362052	.	engineered_region	1009	1869	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K362052	.	CDS	1027	1707	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K362052	.	engineered_tag	1708	1732	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K362052	.	CDS	1027	1701	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K362052	.	terminator	1741	1820	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K362052	.	engineered_region	1741	1820	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K362052	.	stem_loop	1752	1795	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K362052	.	ribosome_entry_site	1009	1020	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K362052	.	conserved_region	1013	1016	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K362052	.	terminator	1829	1869	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K362052	.	stop_codon	1862	1862	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K362052	.	polyA_site	1856	1869	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K362052	.	stem_loop	1836	1855	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K362052	.	engineered_region	1829	1869	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K362052	.	promoter	1	1000	.	+	0	ID=BBa_K362001;Name=BBa_K362001
BBa_K362052	.	terminator	3777	3816	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K362052	.	stop_codon	3810	3810	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K362052	.	polyA_site	3804	3817	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K362052	.	stem_loop	3784	3803	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K362052	.	engineered_region	3777	3817	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K362052	.	promoter	1878	2877	.	+	0	ID=BBa_K362005;Name=BBa_K362005
>BBa_K362052
tccatcagtttctcattgtcacttaacactttcatttgctctgtacttacgtttgcaggcacgtttagcttaagcttgc
caccagaggcttcatattgttgccaggtttttgcgggatctttggaggcaagaattgccgctgctgtcgccgggctttc
tggcttgataaccccaaccaacaatgttcttaattgcactttgccagaatctacccacgggcgcgcctgctgccagaac
tgtttacaatatgggcagaacggatcggcgaagacgtagacaatgaccggcgcatcttttttaccgtcgaggagccagt
gggattgttccatccgttgccacatttcgcgtccggctggtgcgtaaatttctttttcgataagtgtgttactcaggtt
ttcacctttctcgttgtacatgtaaccagagatagcgtgcttaccatctggagtcaggtagatggtgacgcccatatcc
tgatactttccgagataacctttcattcctccgggggcatcgaatgttttgatgattgtaatgccctgtttttcaatcg
cttttactggagcaggaagttcctctgcgaaggcgattgcaggaagcagagccagtaaaagtatcttttttaacattaa
tttgtccttttcagtcagtgcaaaagtcgagtaaaaggcataacctatcactgtcataggtaagagcttagatcaggtg
attgccctttgtttatgagggtgttgtaatccatgtcgttgttgcatttgtaagggcaacacctcagcctgcaggcagg
cactgaagataccaaagggtagttcagattacacggtcacctggaaagggggccattttactttttatcgccgctggcg
gtgcaaagttcacaaagttgtcttacgaaggttgtaaggtaaaacttatcgatttgataatggaaacgcattagccgaa
tcggcaaaaattggttaccttacatctcatcgaaaacacggaggaagtatagtactagagaaagaggagaaatactaga
tggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagtt
cgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccg
ctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcc
cggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttac
cgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaag
gtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaa
accggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgtt
gaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctggaaatcaatgctaacc
acgtccgctcggcacgagaagggcgggtgcgcggcgtatgcaaaccgttgcatctcggttcgcgtcaccaggtctggca
gattgaaatcttcgatgagaaagggcgtttgtgctgttcgtcacgattgacgaccgccattttgtgacgttgtcaccag
aaaagtgtgacgcatattgcttttggtcattatctgcacagtaaacggtggtatactgtgcaggtcttgtctaaaagcg
aggatgctatgtctactcaacttgatcccacccagctggccattgaatttttacgccgtgaccagagcaatctttcacc
tgcccaatatctcaaacgtctgaaacaactagaacttgaatttgctgacctgcttactctctcttctgctgaattaaaa
gaagaaatctactttgcctggcgtctgggcgtgcattgatcctgacttaatgccgcaagttcgccaaatcgacctggat
taacctccgtccaggttcgatgaccttcttaatactgcttatctaccgcctgatattgttcaaaaataatactaatcgc
agtttgtgttaaaacggcgggttagctttatgagattattcttatcgccccttcaagagctaagccactgagagtgccg
gagataagcgccggatggggtagaaacccttaagcctgtgtcgcacagacttaagggttttcttatttctgaacgctgc
acttttttacctatctcttaaatagtcattttcaatacaactttttctttttcatcacagccttaatgctccttagata
ataaccattatctaacaatgagatacctaattcttagcgtgcctgttaacccacacatcagggtctatgcttattaaaa
gcaggcaaaaagcatgttttacggtaaagcccctgcgtttgctgggttgaactgataatcattatcactaacatgctgt
tatacgctgaaagcgatgaagtgaggtaaatcgtactagaggtcgactgacgactggatcctatcaagatctgtcggat
aacgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattc
ttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaa
acttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggt
gttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtac
aggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgt
taatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataac
tcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaag
atggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaacca
ttacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaaca
gctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttata