BBa_K362052
type: ComponentDefinition
persistent identity: BBa K362052
display id: BBa_K362052
version: 1
was generated by: iGEM to SBOL conversion
description: ahpC promoter - RBS - RFP - Term - Term - sufA promoter - RBS - GFP - Term - Term
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:13Z
mutable provenance: sufA Promoter from E.coli genome
owned by: james
owned by: myers
mutable description: This composite part contains ahpC promoter(BBa_K362001),RBS(BBa_K121013),GFP(BBa_K121013),2 terminators(BBa_K121013),sufA promoter(BBa_K362005),RBS(BBa_I13507),RFP(BBa_I13507) and 2 terminators(BBa_I13507). sufA promoter has sensitibity to oxydative stress. ahpC promoter is controlled by OxyR protein, an oxidative stress resoponsive transcription facor. sufA promoter has sensitivity to oxidative stress.
discontinued: false
dominant: false
experience: experience/None
group u list: _480_
m user id: 0
owner id: 7848
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This composite part contains ahpC promoter(BBa_K362001),RBS(BBa_K121013),GFP(BBa_K121013),2 terminators(BBa_K121013),sufA promoter(BBa_K362005),RBS(BBa_I13507),RFP(BBa_I13507) and 2 terminators(BBa_I13507). sufA promoter has sensitibity to oxydative stress. ahpC promoter is controlled by OxyR protein, an oxidative stress resoponsive transcription facor. sufA promoter has sensitivity to oxidative stress.
star: false
creator: Fumihiko Takeuchi, Aya Iwaki, Akiko Kawashima , Yuki Fukuyama
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0010
component: BBa_J54103
component: BBa_K362005
component: BBa_I13507
component: BBa_K121013
component: BBa_B0012
component: BBa_K362001
sequence annotation: BBa_K362001
sequence annotation: BBa_I13507
sequence annotation: BBa_K362005
sequence annotation: BBa_J54103
sequence annotation: BBa_K121013
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K362052_sequence/1

BBa_B0010
type: Component
persistent identity: component2102086
display id: component2102086
version: 1
top level: BBa_K362052
definition: BBa_B0010
access: public

BBa_J54103
type: Component
persistent identity: component2102076
display id: component2102076
version: 1
top level: BBa_K362052
definition: BBa_J54103
access: public

BBa_K362005
type: Component
persistent identity: component2102074
display id: component2102074
version: 1
top level: BBa_K362052
definition: BBa_K362005
access: public

BBa_I13507
type: Component
persistent identity: component2102073
display id: component2102073
version: 1
top level: BBa_K362052
definition: BBa_I13507
access: public

BBa_K121013
type: Component
persistent identity: component2102092
display id: component2102092
version: 1
top level: BBa_K362052
definition: BBa_K121013
access: public

BBa_B0012
type: Component
persistent identity: component2102088
display id: component2102088
version: 1
top level: BBa_K362052
definition: BBa_B0012
access: public

BBa_K362001
type: Component
persistent identity: component2102061
display id: component2102061
version: 1
top level: BBa_K362052
definition: BBa_K362001
access: public

BBa_K362001
type: SequenceAnnotation
persistent identity: annotation2102061
display id: annotation2102061
version: 1
top level: BBa_K362052
location: https://synbiohub.org/public/igem/BBa_K362052/annotation2102061/range2102061/1
component: BBa_K362001

https://synbiohub.org/public/igem/BBa_K362052/annotation2102061/range2102061/1
type: Range
persistent identity: range2102061
display id: range2102061
version: 1
top level: BBa_K362052
direction: direction/reverse
start: 1
end: 1000
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2102073
display id: annotation2102073
version: 1
top level: BBa_K362052
location: https://synbiohub.org/public/igem/BBa_K362052/annotation2102073/range2102073/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K362052/annotation2102073/range2102073/1
type: Range
persistent identity: range2102073
display id: range2102073
version: 1
top level: BBa_K362052
direction: direction/reverse
start: 1009
end: 1869
orientation: inline

BBa_K362005
type: SequenceAnnotation
persistent identity: annotation2102074
display id: annotation2102074
version: 1
top level: BBa_K362052
location: https://synbiohub.org/public/igem/BBa_K362052/annotation2102074/range2102074/1
component: BBa_K362005

https://synbiohub.org/public/igem/BBa_K362052/annotation2102074/range2102074/1
type: Range
persistent identity: range2102074
display id: range2102074
version: 1
top level: BBa_K362052
direction: direction/reverse
start: 1878
end: 2877
orientation: inline

BBa_J54103
type: SequenceAnnotation
persistent identity: annotation2102076
display id: annotation2102076
version: 1
top level: BBa_K362052
location: https://synbiohub.org/public/igem/BBa_K362052/annotation2102076/range2102076/1
component: BBa_J54103

https://synbiohub.org/public/igem/BBa_K362052/annotation2102076/range2102076/1
type: Range
persistent identity: range2102076
display id: range2102076
version: 1
top level: BBa_K362052
direction: direction/reverse
start: 2886
end: 3816
orientation: inline

BBa_K121013
type: SequenceAnnotation
persistent identity: annotation2102092
display id: annotation2102092
version: 1
top level: BBa_K362052
location: https://synbiohub.org/public/igem/BBa_K362052/annotation2102092/range2102092/1
component: BBa_K121013

https://synbiohub.org/public/igem/BBa_K362052/annotation2102092/range2102092/1
type: Range
persistent identity: range2102092
display id: range2102092
version: 1
top level: BBa_K362052
direction: direction/reverse
start: 2886
end: 3816
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2102086
display id: annotation2102086
version: 1
top level: BBa_K362052
location: https://synbiohub.org/public/igem/BBa_K362052/annotation2102086/range2102086/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K362052/annotation2102086/range2102086/1
type: Range
persistent identity: range2102086
display id: range2102086
version: 1
top level: BBa_K362052
direction: direction/forward
start: 3689
end: 3768
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2102088
display id: annotation2102088
version: 1
top level: BBa_K362052
location: https://synbiohub.org/public/igem/BBa_K362052/annotation2102088/range2102088/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K362052/annotation2102088/range2102088/1
type: Range
persistent identity: range2102088
display id: range2102088
version: 1
top level: BBa_K362052
direction: direction/forward
start: 3777
end: 3816
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_I13507
type: ComponentDefinition
persistent identity: BBa I13507
display id: BBa_I13507
version: 1
was generated by: iGEM to SBOL conversion
description: Screening plasmid intermediate
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Built by Josh as an intermediate in screening plasmid construction.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13507_sequence/1

BBa_B0034
type: Component
persistent identity: component1524815
display id: component1524815
version: 1
top level: BBa_I13507
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1524828
display id: component1524828
version: 1
top level: BBa_I13507
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component1524822
display id: component1524822
version: 1
top level: BBa_I13507
definition: mRFP1
access: public

BBa_B0012
type: Component
persistent identity: component1524838
display id: component1524838
version: 1
top level: BBa_I13507
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1524815
display id: annotation1524815
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
type: Range
persistent identity: range1524815
display id: range1524815
version: 1
top level: BBa_I13507
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1524822
display id: annotation1524822
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
type: Range
persistent identity: range1524822
display id: range1524822
version: 1
top level: BBa_I13507
direction: direction/forward
start: 19
end: 699
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1524828
display id: annotation1524828
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
type: Range
persistent identity: range1524828
display id: range1524828
version: 1
top level: BBa_I13507
direction: direction/forward
start: 733
end: 812
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1524838
display id: annotation1524838
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
type: Range
persistent identity: range1524838
display id: range1524838
version: 1
top level: BBa_I13507
direction: direction/forward
start: 821
end: 861
orientation: inline

BBa_J54103
type: ComponentDefinition
persistent identity: BBa J54103
display id: BBa_J54103
version: 1
was generated by: iGEM to SBOL conversion
description: Promoterless-GFP(A)
created: 2006-10-16T11:00:00Z
modified: 2015-08-31T03:48:11Z
mutable provenance: synthesis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: promoterless
discontinued: false
dominant: true
experience: experience/None
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: In stock
status: status/Available
bookmark: true
mutable notes: as negative control
star: true
creator: Yusaku Nakashima
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_J54033
sequence annotation: SalI
sequence annotation: BamHI
sequence annotation: BglII
sequence annotation: BBa_J54025
sequence annotation: MulI
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence: https://synbiohub.org/public/igem/BBa_J54103_sequence/1

BBa_J54033
type: SequenceAnnotation
persistent identity: annotation2152489
display id: annotation2152489
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152489/range2152489/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_J54103/annotation2152489/range2152489/1
type: Range
persistent identity: range2152489
display id: range2152489
version: 1
top level: BBa_J54103
direction: direction/reverse
start: 1
end: 20
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation2152490
display id: annotation2152490
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152490/range2152490/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J54103/annotation2152490/range2152490/1
type: Range
persistent identity: range2152490
display id: range2152490
version: 1
top level: BBa_J54103
direction: direction/forward
start: 1
end: 6
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation2152491
display id: annotation2152491
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152491/range2152491/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J54103/annotation2152491/range2152491/1
type: Range
persistent identity: range2152491
display id: range2152491
version: 1
top level: BBa_J54103
direction: direction/forward
start: 15
end: 20
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation2152493
display id: annotation2152493
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152493/range2152493/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J54103/annotation2152493/range2152493/1
type: Range
persistent identity: range2152493
display id: range2152493
version: 1
top level: BBa_J54103
direction: direction/forward
start: 27
end: 32
orientation: inline

BBa_J54025
type: SequenceAnnotation
persistent identity: annotation2152492
display id: annotation2152492
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152492/range2152492/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J54103/annotation2152492/range2152492/1
type: Range
persistent identity: range2152492
display id: range2152492
version: 1
top level: BBa_J54103
direction: direction/reverse
start: 27
end: 46
orientation: inline

MulI
type: SequenceAnnotation
persistent identity: annotation2152494
display id: annotation2152494
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152494/range2152494/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J54103/annotation2152494/range2152494/1
type: Range
persistent identity: range2152494
display id: range2152494
version: 1
top level: BBa_J54103
direction: direction/forward
start: 41
end: 46
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2152496
display id: annotation2152496
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152496/range2152496/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J54103/annotation2152496/range2152496/1
type: Range
persistent identity: range2152496
display id: range2152496
version: 1
top level: BBa_J54103
direction: direction/forward
start: 49
end: 64
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation2152495
display id: annotation2152495
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152495/range2152495/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J54103/annotation2152495/range2152495/1
type: Range
persistent identity: range2152495
display id: range2152495
version: 1
top level: BBa_J54103
direction: direction/forward
start: 49
end: 64
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2152497
display id: annotation2152497
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152497/range2152497/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J54103/annotation2152497/range2152497/1
type: Range
persistent identity: range2152497
display id: range2152497
version: 1
top level: BBa_J54103
direction: direction/forward
start: 56
end: 62
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2152498
display id: annotation2152498
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152498/range2152498/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_J54103/annotation2152498/range2152498/1
type: Range
persistent identity: range2152498
display id: range2152498
version: 1
top level: BBa_J54103
direction: direction/reverse
start: 71
end: 790
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2152499
display id: annotation2152499
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152499/range2152499/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_J54103/annotation2152499/range2152499/1
type: Range
persistent identity: range2152499
display id: range2152499
version: 1
top level: BBa_J54103
direction: direction/forward
start: 800
end: 879
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2152501
display id: annotation2152501
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152501/range2152501/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_J54103/annotation2152501/range2152501/1
type: Range
persistent identity: range2152501
display id: range2152501
version: 1
top level: BBa_J54103
direction: direction/forward
start: 811
end: 854
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2152500
display id: annotation2152500
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152500/range2152500/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J54103/annotation2152500/range2152500/1
type: Range
persistent identity: range2152500
display id: range2152500
version: 1
top level: BBa_J54103
direction: direction/forward
start: 887
end: 927
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2152502
display id: annotation2152502
version: 1
top level: BBa_J54103
location: https://synbiohub.org/public/igem/BBa_J54103/annotation2152502/range2152502/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_J54103/annotation2152502/range2152502/1
type: Range
persistent identity: range2152502
display id: range2152502
version: 1
top level: BBa_J54103
direction: direction/forward
start: 899
end: 918
orientation: inline

BBa_K121013
type: ComponentDefinition
persistent identity: BBa K121013
display id: BBa_K121013
version: 1
was generated by: iGEM to SBOL conversion
description: Promoterless GFP on pSB6
created: 2008-10-15T11:00:00Z
modified: 2015-05-08T01:09:41Z
mutable provenance: na
owned by: james
owned by: myers
mutable description: Promoterless GFP reporter
discontinued: false
dominant: false
experience: experience/Works
group u list: _220_
m user id: 0
owner id: 3180
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: na
star: false
creator: Masashi Kajita
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_J54033
sequence annotation: SalI
sequence annotation: BBa_J54103
sequence annotation: BamHI
sequence annotation: BglII
sequence annotation: BBa_J54025
sequence annotation: MulI
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K121013_sequence/1

BBa_J54033
type: SequenceAnnotation
persistent identity: annotation1981702
display id: annotation1981702
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981702/range1981702/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K121013/annotation1981702/range1981702/1
type: Range
persistent identity: range1981702
display id: range1981702
version: 1
top level: BBa_K121013
direction: direction/reverse
start: 1
end: 19
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation1981701
display id: annotation1981701
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981701/range1981701/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K121013/annotation1981701/range1981701/1
type: Range
persistent identity: range1981701
display id: range1981701
version: 1
top level: BBa_K121013
direction: direction/forward
start: 1
end: 5
orientation: inline

BBa_J54103
type: SequenceAnnotation
persistent identity: annotation1981717
display id: annotation1981717
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981717/range1981717/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K121013/annotation1981717/range1981717/1
type: Range
persistent identity: range1981717
display id: range1981717
version: 1
top level: BBa_K121013
direction: direction/reverse
start: 1
end: 931
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1981703
display id: annotation1981703
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981703/range1981703/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K121013/annotation1981703/range1981703/1
type: Range
persistent identity: range1981703
display id: range1981703
version: 1
top level: BBa_K121013
direction: direction/forward
start: 15
end: 19
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1981704
display id: annotation1981704
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981704/range1981704/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K121013/annotation1981704/range1981704/1
type: Range
persistent identity: range1981704
display id: range1981704
version: 1
top level: BBa_K121013
direction: direction/forward
start: 28
end: 32
orientation: inline

BBa_J54025
type: SequenceAnnotation
persistent identity: annotation1981705
display id: annotation1981705
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981705/range1981705/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K121013/annotation1981705/range1981705/1
type: Range
persistent identity: range1981705
display id: range1981705
version: 1
top level: BBa_K121013
direction: direction/reverse
start: 28
end: 46
orientation: inline

MulI
type: SequenceAnnotation
persistent identity: annotation1981706
display id: annotation1981706
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981706/range1981706/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K121013/annotation1981706/range1981706/1
type: Range
persistent identity: range1981706
display id: range1981706
version: 1
top level: BBa_K121013
direction: direction/forward
start: 41
end: 46
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1981708
display id: annotation1981708
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981708/range1981708/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K121013/annotation1981708/range1981708/1
type: Range
persistent identity: range1981708
display id: range1981708
version: 1
top level: BBa_K121013
direction: direction/forward
start: 55
end: 69
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1981707
display id: annotation1981707
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981707/range1981707/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K121013/annotation1981707/range1981707/1
type: Range
persistent identity: range1981707
display id: range1981707
version: 1
top level: BBa_K121013
direction: direction/forward
start: 55
end: 69
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1981709
display id: annotation1981709
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981709/range1981709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K121013/annotation1981709/range1981709/1
type: Range
persistent identity: range1981709
display id: range1981709
version: 1
top level: BBa_K121013
direction: direction/forward
start: 62
end: 66
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1981710
display id: annotation1981710
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981710/range1981710/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K121013/annotation1981710/range1981710/1
type: Range
persistent identity: range1981710
display id: range1981710
version: 1
top level: BBa_K121013
direction: direction/reverse
start: 76
end: 795
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1981711
display id: annotation1981711
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981711/range1981711/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K121013/annotation1981711/range1981711/1
type: Range
persistent identity: range1981711
display id: range1981711
version: 1
top level: BBa_K121013
direction: direction/forward
start: 804
end: 883
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1981712
display id: annotation1981712
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981712/range1981712/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K121013/annotation1981712/range1981712/1
type: Range
persistent identity: range1981712
display id: range1981712
version: 1
top level: BBa_K121013
direction: direction/forward
start: 815
end: 858
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1981713
display id: annotation1981713
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981713/range1981713/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K121013/annotation1981713/range1981713/1
type: Range
persistent identity: range1981713
display id: range1981713
version: 1
top level: BBa_K121013
direction: direction/forward
start: 892
end: 932
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1981714
display id: annotation1981714
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981714/range1981714/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K121013/annotation1981714/range1981714/1
type: Range
persistent identity: range1981714
display id: range1981714
version: 1
top level: BBa_K121013
direction: direction/forward
start: 899
end: 918
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1981715
display id: annotation1981715
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981715/range1981715/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_K121013/annotation1981715/range1981715/1
type: Range
persistent identity: range1981715
display id: range1981715
version: 1
top level: BBa_K121013
direction: direction/forward
start: 919
end: 932
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1981716
display id: annotation1981716
version: 1
top level: BBa_K121013
location: https://synbiohub.org/public/igem/BBa_K121013/annotation1981716/range1981716/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K121013/annotation1981716/range1981716/1
type: Range
persistent identity: range1981716
display id: range1981716
version: 1
top level: BBa_K121013
direction: direction/forward
start: 925
end: 925
orientation: inline

BBa_K362001
type: ComponentDefinition
persistent identity: BBa K362001
display id: BBa_K362001
version: 1
was generated by: iGEM to SBOL conversion
description: ahpC, this promoter is controlled by the OxyR protein.
created: 2010-09-21T11:00:00Z
modified: 2015-05-08T01:12:12Z
mutable provenance: This promoter , Pahpc , exists on the upper stream of ahpC.
owned by: james
owned by: myers
mutable description: This promoter has sensitivity to oxidative stress.
discontinued: false
dominant: false
experience: experience/Works
group u list: _480_
m user id: 0
owner id: 6267
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: TCCATCAGTTTCTCATTGTCACTTAACACTTTCATTTGCTCTGTACTTACGTTTGCAGGCACGTTTAGCTTAAGCTTGCCACCAGAGGCTTCATATTGTTGCCAGGTTTTTGCGGGATCTTTGGAGGCAAGAATTGCCGCTGCTGTCGCCGGGCTTTCTGGCTTGATAACCCCAACCAACAATGTTCTTAATTGCACTTTGCCAGAATCTACCCACGGGCGCGCCTGCTGCCAGAACTGTTTACAATATGGGCAGAACGGATCGGCGAAGACGTAGACAATGACCGGCGCATCTTTTTTACCGTCGAGGAGCCAGTGGGATTGTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCAGATTACACGGTCACCTGGAAAGGGGGCCATTTTACTTTTTATCGCCGCTGGCGGTGCAAAGTTCACAAAGTTGTCTTACGAAGGTTGTAAGGTAAAACTTATCGATTTGATAATGGAAACGCATTAGCCGAATCGGCAAAAATTGGTTACCTTACATCTCATCGAAAACACGGAGGAAGTATAG
star: false
creator: Fumihiko Takeuchi,Aya Iwaki,Yuki Fukuyama,Satori Furumiti, Kazuma Usui, Akiko Kawashima, Toyokazu Nakamura,Reo Yoshimura
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K362001_sequence/1

BBa_K362005
type: ComponentDefinition
persistent identity: BBa K362005
display id: BBa_K362005
version: 1
was generated by: iGEM to SBOL conversion
description: sufA , this promoter is controlled by OxyR.
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:12:12Z
mutable provenance: sufA , this promoter has sensitivity to oxidative stress.
owned by: james
owned by: myers
mutable description: sufA , this promoter has sensitivity to oxidative stress.
discontinued: false
dominant: false
experience: experience/Works
group u list: _480_
m user id: 0
owner id: 6267
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: sufA , this promoter has sensitivity to oxidative stress.
star: false
creator: Fumihiko Takeuchi,Aya Iwaki,Yuki Fukuyama,Satori Furumiti, Kazuma Usui, Akiko Kawashima, Toyokazu Nakamura,Reo Yoshimura
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K362005_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

https://synbiohub.org/public/igem/BBa_K362052_sequence/1
type: Sequence
persistent identity: BBa K362052 sequence
display id: BBa_K362052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccatcagtttctcattgtcacttaacactttcatttgctctgtacttacgtttgcaggcacgtttagcttaagcttgccaccagaggcttcatattgttgccaggtttttgcgggatctttggaggcaagaattgccgctgctgtcgccgggctttctggcttgataaccccaaccaacaatgttcttaattgcactttgccagaatctacccacgggcgcgcctgctgccagaactgtttacaatatgggcagaacggatcggcgaagacgtagacaatgaccggcgcatcttttttaccgtcgaggagccagtgggattgttccatccgttgccacatttcgcgtccggctggtgcgtaaatttctttttcgataagtgtgttactcaggttttcacctttctcgttgtacatgtaaccagagatagcgtgcttaccatctggagtcaggtagatggtgacgcccatatcctgatactttccgagataacctttcattcctccgggggcatcgaatgttttgatgattgtaatgccctgtttttcaatcgcttttactggagcaggaagttcctctgcgaaggcgattgcaggaagcagagccagtaaaagtatcttttttaacattaatttgtccttttcagtcagtgcaaaagtcgagtaaaaggcataacctatcactgtcataggtaagagcttagatcaggtgattgccctttgtttatgagggtgttgtaatccatgtcgttgttgcatttgtaagggcaacacctcagcctgcaggcaggcactgaagataccaaagggtagttcagattacacggtcacctggaaagggggccattttactttttatcgccgctggcggtgcaaagttcacaaagttgtcttacgaaggttgtaaggtaaaacttatcgatttgataatggaaacgcattagccgaatcggcaaaaattggttaccttacatctcatcgaaaacacggaggaagtatagtactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctggaaatcaatgctaaccacgtccgctcggcacgagaagggcgggtgcgcggcgtatgcaaaccgttgcatctcggttcgcgtcaccaggtctggcagattgaaatcttcgatgagaaaggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13507_sequence/1
type: Sequence
persistent identity: BBa I13507 sequence
display id: BBa_I13507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J54103_sequence/1
type: Sequence
persistent identity: BBa J54103 sequence
display id: BBa_J54103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K121013_sequence/1
type: Sequence
persistent identity: BBa K121013 sequence
display id: BBa_K121013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtcgactgacgactggatcctatcaagatctgtcggataacgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K362001_sequence/1
type: Sequence
persistent identity: BBa K362001 sequence
display id: BBa_K362001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccatcagtttctcattgtcacttaacactttcatttgctctgtacttacgtttgcaggcacgtttagcttaagcttgccaccagaggcttcatattgttgccaggtttttgcgggatctttggaggcaagaattgccgctgctgtcgccgggctttctggcttgataaccccaaccaacaatgttcttaattgcactttgccagaatctacccacgggcgcgcctgctgccagaactgtttacaatatgggcagaacggatcggcgaagacgtagacaatgaccggcgcatcttttttaccgtcgaggagccagtgggattgttccatccgttgccacatttcgcgtccggctggtgcgtaaatttctttttcgataagtgtgttactcaggttttcacctttctcgttgtacatgtaaccagagatagcgtgcttaccatctggagtcaggtagatggtgacgcccatatcctgatactttccgagataacctttcattcctccgggggcatcgaatgttttgatgattgtaatgccctgtttttcaatcgcttttactggagcaggaagttcctctgcgaaggcgattgcaggaagcagagccagtaaaagtatcttttttaacattaatttgtccttttcagtcagtgcaaaagtcgagtaaaaggcataacctatcactgtcataggtaagagcttagatcaggtgattgccctttgtttatgagggtgttgtaatccatgtcgttgttgcatttgtaagggcaacacctcagcctgcaggcaggcactgaagataccaaagggtagttcagattacacggtcacctggaaagggggccattttactttttatcgccgctggcggtgcaaagttcacaaagttgtcttacgaaggttgtaaggtaaaacttatcgatttgataatggaaacgcattagccgaatcggcaaaaattggttaccttacatctcatcgaaaacacggaggaagtatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K362005_sequence/1
type: Sequence
persistent identity: BBa K362005 sequence
display id: BBa_K362005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggaaatcaatgctaaccacgtccgctcggcacgagaagggcgggtgcgcggcgtatgcaaaccgttgcatctcggttcgcgtcaccaggtctggcagattgaaatcttcgatgagaaagggcgtttgtgctgttcgtcacgattgacgaccgccattttgtgacgttgtcaccagaaaagtgtgacgcatattgcttttggtcattatctgcacagtaaacggtggtatactgtgcaggtcttgtctaaaagcgaggatgctatgtctactcaacttgatcccacccagctggccattgaatttttacgccgtgaccagagcaatctttcacctgcccaatatctcaaacgtctgaaacaactagaacttgaatttgctgacctgcttactctctcttctgctgaattaaaagaagaaatctactttgcctggcgtctgggcgtgcattgatcctgacttaatgccgcaagttcgccaaatcgacctggattaacctccgtccaggttcgatgaccttcttaatactgcttatctaccgcctgatattgttcaaaaataatactaatcgcagtttgtgttaaaacggcgggttagctttatgagattattcttatcgccccttcaagagctaagccactgagagtgccggagataagcgccggatggggtagaaacccttaagcctgtgtcgcacagacttaagggttttcttatttctgaacgctgcacttttttacctatctcttaaatagtcattttcaatacaactttttctttttcatcacagccttaatgctccttagataataaccattatctaacaatgagatacctaattcttagcgtgcctgttaacccacacatcagggtctatgcttattaaaagcaggcaaaaagcatgttttacggtaaagcccctgcgtttgctgggttgaactgataatcattatcactaacatgctgttatacgctgaaagcgatgaagtgaggtaaatcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z