##gff-version 3
##sequence-region BBa_K362059 1 1266
BBa_K362059	.	engineered_region	336	1266	.	+	0	ID=BBa_J54103;Name=BBa_J54103
BBa_K362059	.	tag	350	355	.	+	0	ID=annotation2152491;Name=BamHI;Parent=BBa_J54103
BBa_K362059	.	engineered_region	1222	1262	.	+	0	ID=annotation2152500;Name=BBa_B0012;Parent=BBa_J54103
BBa_K362059	.	tag	376	381	.	+	0	ID=annotation2152494;Name=MulI;Parent=BBa_J54103
BBa_K362059	.	engineered_region	384	399	.	+	0	ID=annotation2152496;Name=BBa_B0030;Parent=BBa_J54103
BBa_K362059	.	ribosome_entry_site	391	397	.	+	0	ID=annotation2152497;Name=RBS;Parent=BBa_J54103
BBa_K362059	.	ribosome_entry_site	384	399	.	+	0	ID=annotation2152495;Name=RBS-1\Strong;Parent=BBa_J54103
BBa_K362059	.	engineered_region	1135	1214	.	+	0	ID=annotation2152499;Name=BBa_B0010;Parent=BBa_J54103
BBa_K362059	.	stem_loop	1146	1189	.	+	0	ID=annotation2152501;Name=stem_loop;Parent=BBa_J54103
BBa_K362059	.	engineered_region	406	1125	.	+	0	ID=annotation2152498;Name=BBa_E0040;Parent=BBa_J54103
BBa_K362059	.	engineered_region	362	381	.	+	0	ID=annotation2152492;Name=BBa_J54025;Parent=BBa_J54103
BBa_K362059	.	tag	336	341	.	+	0	ID=annotation2152490;Name=SalI;Parent=BBa_J54103
BBa_K362059	.	stem_loop	1234	1253	.	+	0	ID=annotation2152502;Name=T7 TE;Parent=BBa_J54103
BBa_K362059	.	tag	362	367	.	+	0	ID=annotation2152493;Name=BglII;Parent=BBa_J54103
BBa_K362059	.	engineered_region	336	355	.	+	0	ID=annotation2152489;Name=BBa_J54033;Parent=BBa_J54103
BBa_K362059	.	engineered_region	336	1266	.	+	0	ID=BBa_K121013;Name=BBa_K121013
BBa_K362059	.	engineered_region	336	1266	.	+	0	ID=annotation1981717;Name=BBa_J54103;Parent=BBa_K121013
BBa_K362059	.	engineered_region	1139	1218	.	+	0	ID=annotation1981711;Name=BBa_B0010;Parent=BBa_K121013
BBa_K362059	.	polyA_site	1254	1267	.	+	0	ID=annotation1981715;Name=polya;Parent=BBa_K121013
BBa_K362059	.	tag	336	340	.	+	0	ID=annotation1981701;Name=SalI;Parent=BBa_K121013
BBa_K362059	.	stem_loop	1234	1253	.	+	0	ID=annotation1981714;Name=T7 TE;Parent=BBa_K121013
BBa_K362059	.	engineered_region	1227	1267	.	+	0	ID=annotation1981713;Name=BBa_B0012;Parent=BBa_K121013
BBa_K362059	.	tag	350	354	.	+	0	ID=annotation1981703;Name=BamHI;Parent=BBa_K121013
BBa_K362059	.	tag	376	381	.	+	0	ID=annotation1981706;Name=MulI;Parent=BBa_K121013
BBa_K362059	.	stem_loop	1150	1193	.	+	0	ID=annotation1981712;Name=stem_loop;Parent=BBa_K121013
BBa_K362059	.	engineered_region	390	404	.	+	0	ID=annotation1981708;Name=BBa_B0030;Parent=BBa_K121013
BBa_K362059	.	ribosome_entry_site	397	401	.	+	0	ID=annotation1981709;Name=RBS;Parent=BBa_K121013
BBa_K362059	.	tag	363	367	.	+	0	ID=annotation1981704;Name=BglII;Parent=BBa_K121013
BBa_K362059	.	engineered_region	336	354	.	+	0	ID=annotation1981702;Name=BBa_J54033;Parent=BBa_K121013
BBa_K362059	.	engineered_region	363	381	.	+	0	ID=annotation1981705;Name=BBa_J54025;Parent=BBa_K121013
BBa_K362059	.	stop_codon	1260	1260	.	+	0	ID=annotation1981716;Name=stop;Parent=BBa_K121013
BBa_K362059	.	ribosome_entry_site	390	404	.	+	0	ID=annotation1981707;Name=RBS-1\Strong;Parent=BBa_K121013
BBa_K362059	.	engineered_region	411	1130	.	+	0	ID=annotation1981710;Name=BBa_E0040;Parent=BBa_K121013
BBa_K362059	.	promoter	1	327	.	+	0	ID=BBa_K362000;Name=BBa_K362000
>BBa_K362059
gatctcactgaacaaatccgacttgtctttaaaatgccactagattgcaccgcgcgtaacgccagctgctttttgcaat
ctcgcccagcgaggtggatgataccccctgctgtgagaaaagacgtagagccacatcgaggatgtgttggcgcgtttct
tgcgcttcttgtttggtttttcgtgccatatgttcgtgaatttacaggcgttagatttacatacatttgtgaatgtatg
taccatagcacgacgataatataaacgcagcaatgggtttattaacttttgaccattgaccaatttgaaatcggacact
cgaggtttacatactagaggtcgactgacgactggatcctatcaagatctgtcggataacgcgttactagagattaaag
aggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgt
taatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgc
actactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacc
cagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaa
agatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggt
attgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatgg
cagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcaga
ccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgcc
ctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggca
tggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
ta