##gff-version 3
##sequence-region BBa_K362060 1 1439
BBa_K362060	.	engineered_region	509	1439	.	+	0	ID=BBa_J54103;Name=BBa_J54103
BBa_K362060	.	tag	549	554	.	+	0	ID=annotation2152494;Name=MulI;Parent=BBa_J54103
BBa_K362060	.	engineered_region	1395	1435	.	+	0	ID=annotation2152500;Name=BBa_B0012;Parent=BBa_J54103
BBa_K362060	.	engineered_region	535	554	.	+	0	ID=annotation2152492;Name=BBa_J54025;Parent=BBa_J54103
BBa_K362060	.	ribosome_entry_site	557	572	.	+	0	ID=annotation2152495;Name=RBS-1\Strong;Parent=BBa_J54103
BBa_K362060	.	engineered_region	557	572	.	+	0	ID=annotation2152496;Name=BBa_B0030;Parent=BBa_J54103
BBa_K362060	.	engineered_region	1308	1387	.	+	0	ID=annotation2152499;Name=BBa_B0010;Parent=BBa_J54103
BBa_K362060	.	stem_loop	1319	1362	.	+	0	ID=annotation2152501;Name=stem_loop;Parent=BBa_J54103
BBa_K362060	.	engineered_region	509	528	.	+	0	ID=annotation2152489;Name=BBa_J54033;Parent=BBa_J54103
BBa_K362060	.	engineered_region	579	1298	.	+	0	ID=annotation2152498;Name=BBa_E0040;Parent=BBa_J54103
BBa_K362060	.	tag	523	528	.	+	0	ID=annotation2152491;Name=BamHI;Parent=BBa_J54103
BBa_K362060	.	tag	509	514	.	+	0	ID=annotation2152490;Name=SalI;Parent=BBa_J54103
BBa_K362060	.	stem_loop	1407	1426	.	+	0	ID=annotation2152502;Name=T7 TE;Parent=BBa_J54103
BBa_K362060	.	tag	535	540	.	+	0	ID=annotation2152493;Name=BglII;Parent=BBa_J54103
BBa_K362060	.	ribosome_entry_site	564	570	.	+	0	ID=annotation2152497;Name=RBS;Parent=BBa_J54103
BBa_K362060	.	engineered_region	509	1439	.	+	0	ID=BBa_K121013;Name=BBa_K121013
BBa_K362060	.	stop_codon	1433	1433	.	+	0	ID=annotation1981716;Name=stop;Parent=BBa_K121013
BBa_K362060	.	engineered_region	509	1439	.	+	0	ID=annotation1981717;Name=BBa_J54103;Parent=BBa_K121013
BBa_K362060	.	engineered_region	1400	1440	.	+	0	ID=annotation1981713;Name=BBa_B0012;Parent=BBa_K121013
BBa_K362060	.	tag	549	554	.	+	0	ID=annotation1981706;Name=MulI;Parent=BBa_K121013
BBa_K362060	.	engineered_region	563	577	.	+	0	ID=annotation1981708;Name=BBa_B0030;Parent=BBa_K121013
BBa_K362060	.	engineered_region	1312	1391	.	+	0	ID=annotation1981711;Name=BBa_B0010;Parent=BBa_K121013
BBa_K362060	.	engineered_region	536	554	.	+	0	ID=annotation1981705;Name=BBa_J54025;Parent=BBa_K121013
BBa_K362060	.	tag	523	527	.	+	0	ID=annotation1981703;Name=BamHI;Parent=BBa_K121013
BBa_K362060	.	polyA_site	1427	1440	.	+	0	ID=annotation1981715;Name=polya;Parent=BBa_K121013
BBa_K362060	.	stem_loop	1323	1366	.	+	0	ID=annotation1981712;Name=stem_loop;Parent=BBa_K121013
BBa_K362060	.	ribosome_entry_site	563	577	.	+	0	ID=annotation1981707;Name=RBS-1\Strong;Parent=BBa_K121013
BBa_K362060	.	stem_loop	1407	1426	.	+	0	ID=annotation1981714;Name=T7 TE;Parent=BBa_K121013
BBa_K362060	.	tag	536	540	.	+	0	ID=annotation1981704;Name=BglII;Parent=BBa_K121013
BBa_K362060	.	ribosome_entry_site	570	574	.	+	0	ID=annotation1981709;Name=RBS;Parent=BBa_K121013
BBa_K362060	.	tag	509	513	.	+	0	ID=annotation1981701;Name=SalI;Parent=BBa_K121013
BBa_K362060	.	engineered_region	509	527	.	+	0	ID=annotation1981702;Name=BBa_J54033;Parent=BBa_K121013
BBa_K362060	.	engineered_region	584	1303	.	+	0	ID=annotation1981710;Name=BBa_E0040;Parent=BBa_K121013
BBa_K362060	.	promoter	1	500	.	+	0	ID=BBa_K362002;Name=BBa_K362002
>BBa_K362060
cctcacccgtttgccaacacggacatttggtacgctgatgagcatggaaccggcgctggctgccgtttccgggatgatt
ttcctcggagaaacactgacacccatacagctactggcgctcggcgctatcatcgccgcttcaatggggtctacgctga
cagtacgcaaagagagcaaaataaaagaattagacattaattaaatttacatttctgcatggttatgcataaccatgca
gaatttctcgctacttttcctctacaccgtctttatatatcgaattatgcaaaagcatatttattccgaaaattcctgg
cgagcagataaataagaattgttcttatcaatatatctaactcattgaatctttattagttttgtttttcacgcttgtt
accactattagtgtgataggaacagccagaatagcggaacacatagccggtgctatacttaatctcgttaattactggg
acataacatcaagaggatatgaaatttactagaggtcgactgacgactggatcctatcaagatctgtcggataacgcgt
tactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttga
attagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacc
cttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaat
gctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaag
aactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaataga
atcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacaca
atgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaag
cgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctg
tccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctg
ggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata