LOCUS       BBa_K362062             1939 bp    DNA     linear       21-May-2024
DEFINITION  sufA promoter - RBS - GFP - Term - Term
ACCESSION   BBa_K362062
VERSION     BBa_K362062.1  
FEATURES             Location/Qualifiers
     promoter        1..1000
                     /label=BBa_K362005
     misc_feature    1009..1028
                     /label=BBa_J54033
     misc_feature    1009..1014
                     /label=SalI
     misc_feature    1023..1028
                     /label=BamHI
     misc_feature    1035..1054
                     /label=BBa_J54025
     misc_feature    1035..1040
                     /label=BglII
     misc_feature    1049..1054
                     /label=MulI
     RBS             1057..1072
                     /label=RBS-1\Strong
     misc_feature    1057..1072
                     /label=BBa_B0030
     RBS             1064..1070
                     /label=RBS
     misc_feature    1079..1798
                     /label=BBa_E0040
     misc_feature    1808..1887
                     /label=BBa_B0010
     misc_feature    1895..1935
                     /label=BBa_B0012
     stem_loop       1819..1862
                     /label=stem_loop
     stem_loop       1907..1926
                     /label=T7 TE
     misc_feature    1009..1939
                     /label=BBa_J54103
     misc_feature    1009..1013
                     /label=SalI
     misc_feature    1009..1027
                     /label=BBa_J54033
     misc_feature    1023..1027
                     /label=BamHI
     misc_feature    1036..1040
                     /label=BglII
     misc_feature    1036..1054
                     /label=BBa_J54025
     misc_feature    1049..1054
                     /label=MulI
     RBS             1063..1077
                     /label=RBS-1\Strong
     misc_feature    1063..1077
                     /label=BBa_B0030
     RBS             1070..1074
                     /label=RBS
     misc_feature    1084..1803
                     /label=BBa_E0040
     misc_feature    1812..1891
                     /label=BBa_B0010
     stem_loop       1823..1866
                     /label=stem_loop
     misc_feature    1900..1940
                     /label=BBa_B0012
     stem_loop       1907..1926
                     /label=T7 TE
     polyA_site      1927..1940
                     /label=polya
     misc_feature    1933..1933
                     /label=stop
     misc_feature    1009..1939
                     /label=BBa_J54103
     misc_feature    1009..1939
                     /label=BBa_K121013
ORIGIN
        1 ctggaaatca atgctaacca cgtccgctcg gcacgagaag ggcgggtgcg cggcgtatgc
       61 aaaccgttgc atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa
      121 gggcgtttgt gctgttcgtc acgattgacg accgccattt tgtgacgttg tcaccagaaa
      181 agtgtgacgc atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag
      241 gtcttgtcta aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt
      301 gaatttttac gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa
      361 caactagaac ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa
      421 atctactttg cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca
      481 aatcgacctg gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc
      541 gcctgatatt gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt
      601 tatgagatta ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc
      661 gccggatggg gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc
      721 tgaacgctgc acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt
      781 ttcatcacag ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat
      841 tcttagcgtg cctgttaacc cacacatcag ggtctatgct tattaaaagc aggcaaaaag
      901 catgttttac ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac
      961 atgctgttat acgctgaaag cgatgaagtg aggtaaatcg tactagaggt cgactgacga
     1021 ctggatccta tcaagatctg tcggataacg cgttactaga gattaaagag gagaaatact
     1081 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     1141 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     1201 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
     1261 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
     1321 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
     1381 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
     1441 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     1501 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     1561 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     1621 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     1681 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     1741 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     1801 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1861 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1921 gggcctttct gcgtttata
//