##gff-version 3
##sequence-region BBa_K362065 1 5210
BBa_K362065	.	engineered_region	1009	1939	.	+	0	ID=BBa_J54103;Name=BBa_J54103
BBa_K362065	.	engineered_region	1009	1028	.	+	0	ID=annotation2152489;Name=BBa_J54033;Parent=BBa_J54103
BBa_K362065	.	engineered_region	1035	1054	.	+	0	ID=annotation2152492;Name=BBa_J54025;Parent=BBa_J54103
BBa_K362065	.	stem_loop	1907	1926	.	+	0	ID=annotation2152502;Name=T7 TE;Parent=BBa_J54103
BBa_K362065	.	tag	1009	1014	.	+	0	ID=annotation2152490;Name=SalI;Parent=BBa_J54103
BBa_K362065	.	tag	1035	1040	.	+	0	ID=annotation2152493;Name=BglII;Parent=BBa_J54103
BBa_K362065	.	ribosome_entry_site	1064	1070	.	+	0	ID=annotation2152497;Name=RBS;Parent=BBa_J54103
BBa_K362065	.	engineered_region	1808	1887	.	+	0	ID=annotation2152499;Name=BBa_B0010;Parent=BBa_J54103
BBa_K362065	.	tag	1023	1028	.	+	0	ID=annotation2152491;Name=BamHI;Parent=BBa_J54103
BBa_K362065	.	engineered_region	1079	1798	.	+	0	ID=annotation2152498;Name=BBa_E0040;Parent=BBa_J54103
BBa_K362065	.	stem_loop	1819	1862	.	+	0	ID=annotation2152501;Name=stem_loop;Parent=BBa_J54103
BBa_K362065	.	engineered_region	1057	1072	.	+	0	ID=annotation2152496;Name=BBa_B0030;Parent=BBa_J54103
BBa_K362065	.	ribosome_entry_site	1057	1072	.	+	0	ID=annotation2152495;Name=RBS-1\Strong;Parent=BBa_J54103
BBa_K362065	.	tag	1049	1054	.	+	0	ID=annotation2152494;Name=MulI;Parent=BBa_J54103
BBa_K362065	.	engineered_region	1895	1935	.	+	0	ID=annotation2152500;Name=BBa_B0012;Parent=BBa_J54103
BBa_K362065	.	promoter	1948	2947	.	+	0	ID=BBa_K362005;Name=BBa_K362005
BBa_K362065	.	terminator	1812	1891	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K362065	.	engineered_region	1812	1891	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K362065	.	stem_loop	1823	1866	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K362065	.	promoter	3825	4324	.	+	0	ID=BBa_K362002;Name=BBa_K362002
BBa_K362065	.	engineered_region	1009	1939	.	+	0	ID=BBa_K121013;Name=BBa_K121013
BBa_K362065	.	stem_loop	1907	1926	.	+	0	ID=annotation1981714;Name=T7 TE;Parent=BBa_K121013
BBa_K362065	.	engineered_region	1812	1891	.	+	0	ID=annotation1981711;Name=BBa_B0010;Parent=BBa_K121013
BBa_K362065	.	tag	1023	1027	.	+	0	ID=annotation1981703;Name=BamHI;Parent=BBa_K121013
BBa_K362065	.	tag	1049	1054	.	+	0	ID=annotation1981706;Name=MulI;Parent=BBa_K121013
BBa_K362065	.	engineered_region	1063	1077	.	+	0	ID=annotation1981708;Name=BBa_B0030;Parent=BBa_K121013
BBa_K362065	.	ribosome_entry_site	1070	1074	.	+	0	ID=annotation1981709;Name=RBS;Parent=BBa_K121013
BBa_K362065	.	engineered_region	1900	1940	.	+	0	ID=annotation1981713;Name=BBa_B0012;Parent=BBa_K121013
BBa_K362065	.	engineered_region	1036	1054	.	+	0	ID=annotation1981705;Name=BBa_J54025;Parent=BBa_K121013
BBa_K362065	.	stop_codon	1933	1933	.	+	0	ID=annotation1981716;Name=stop;Parent=BBa_K121013
BBa_K362065	.	tag	1036	1040	.	+	0	ID=annotation1981704;Name=BglII;Parent=BBa_K121013
BBa_K362065	.	engineered_region	1009	1939	.	+	0	ID=annotation1981717;Name=BBa_J54103;Parent=BBa_K121013
BBa_K362065	.	stem_loop	1823	1866	.	+	0	ID=annotation1981712;Name=stem_loop;Parent=BBa_K121013
BBa_K362065	.	tag	1009	1013	.	+	0	ID=annotation1981701;Name=SalI;Parent=BBa_K121013
BBa_K362065	.	ribosome_entry_site	1063	1077	.	+	0	ID=annotation1981707;Name=RBS-1\Strong;Parent=BBa_K121013
BBa_K362065	.	polyA_site	1927	1940	.	+	0	ID=annotation1981715;Name=polya;Parent=BBa_K121013
BBa_K362065	.	engineered_region	1084	1803	.	+	0	ID=annotation1981710;Name=BBa_E0040;Parent=BBa_K121013
BBa_K362065	.	engineered_region	1009	1027	.	+	0	ID=annotation1981702;Name=BBa_J54033;Parent=BBa_K121013
BBa_K362065	.	engineered_region	2956	3816	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K362065	.	CDS	2974	3654	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K362065	.	engineered_tag	3655	3679	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K362065	.	CDS	2974	3648	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K362065	.	terminator	3688	3767	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K362065	.	engineered_region	3688	3767	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K362065	.	stem_loop	3699	3742	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K362065	.	ribosome_entry_site	2956	2967	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K362065	.	conserved_region	2960	2963	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K362065	.	terminator	3776	3816	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K362065	.	polyA_site	3803	3816	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K362065	.	stop_codon	3809	3809	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K362065	.	engineered_region	3776	3816	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K362065	.	stem_loop	3783	3802	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K362065	.	promoter	1	1000	.	+	0	ID=BBa_K362001;Name=BBa_K362001
BBa_K362065	.	terminator	1900	1939	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K362065	.	polyA_site	1927	1940	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K362065	.	stop_codon	1933	1933	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K362065	.	engineered_region	1900	1940	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K362065	.	stem_loop	1907	1926	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K362065	.	engineered_region	4333	5210	.	+	0	ID=BBa_E0420;Name=BBa_E0420
BBa_K362065	.	terminator	5082	5161	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0420
BBa_K362065	.	engineered_region	5082	5161	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K362065	.	stem_loop	5093	5136	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K362065	.	terminator	5170	5210	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0420
BBa_K362065	.	polyA_site	5197	5210	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K362065	.	stop_codon	5203	5203	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K362065	.	engineered_region	5170	5210	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K362065	.	stem_loop	5177	5196	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K362065	.	ribosome_entry_site	4333	4344	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0420
BBa_K362065	.	conserved_region	4337	4340	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K362065	.	CDS	4351	5073	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_E0420
>BBa_K362065
tccatcagtttctcattgtcacttaacactttcatttgctctgtacttacgtttgcaggcacgtttagcttaagcttgc
caccagaggcttcatattgttgccaggtttttgcgggatctttggaggcaagaattgccgctgctgtcgccgggctttc
tggcttgataaccccaaccaacaatgttcttaattgcactttgccagaatctacccacgggcgcgcctgctgccagaac
tgtttacaatatgggcagaacggatcggcgaagacgtagacaatgaccggcgcatcttttttaccgtcgaggagccagt
gggattgttccatccgttgccacatttcgcgtccggctggtgcgtaaatttctttttcgataagtgtgttactcaggtt
ttcacctttctcgttgtacatgtaaccagagatagcgtgcttaccatctggagtcaggtagatggtgacgcccatatcc
tgatactttccgagataacctttcattcctccgggggcatcgaatgttttgatgattgtaatgccctgtttttcaatcg
cttttactggagcaggaagttcctctgcgaaggcgattgcaggaagcagagccagtaaaagtatcttttttaacattaa
tttgtccttttcagtcagtgcaaaagtcgagtaaaaggcataacctatcactgtcataggtaagagcttagatcaggtg
attgccctttgtttatgagggtgttgtaatccatgtcgttgttgcatttgtaagggcaacacctcagcctgcaggcagg
cactgaagataccaaagggtagttcagattacacggtcacctggaaagggggccattttactttttatcgccgctggcg
gtgcaaagttcacaaagttgtcttacgaaggttgtaaggtaaaacttatcgatttgataatggaaacgcattagccgaa
tcggcaaaaattggttaccttacatctcatcgaaaacacggaggaagtatagtactagaggtcgactgacgactggatc
ctatcaagatctgtcggataacgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttc
actggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaag
gtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgt
cactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgcc
atgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagt
ttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaa
attggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaa
attagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctg
tccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggt
ccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctggaaatcaatgctaaccacgtccgct
cggcacgagaagggcgggtgcgcggcgtatgcaaaccgttgcatctcggttcgcgtcaccaggtctggcagattgaaat
cttcgatgagaaagggcgtttgtgctgttcgtcacgattgacgaccgccattttgtgacgttgtcaccagaaaagtgtg
acgcatattgcttttggtcattatctgcacagtaaacggtggtatactgtgcaggtcttgtctaaaagcgaggatgcta
tgtctactcaacttgatcccacccagctggccattgaatttttacgccgtgaccagagcaatctttcacctgcccaata
tctcaaacgtctgaaacaactagaacttgaatttgctgacctgcttactctctcttctgctgaattaaaagaagaaatc
tactttgcctggcgtctgggcgtgcattgatcctgacttaatgccgcaagttcgccaaatcgacctggattaacctccg
tccaggttcgatgaccttcttaatactgcttatctaccgcctgatattgttcaaaaataatactaatcgcagtttgtgt
taaaacggcgggttagctttatgagattattcttatcgccccttcaagagctaagccactgagagtgccggagataagc
gccggatggggtagaaacccttaagcctgtgtcgcacagacttaagggttttcttatttctgaacgctgcactttttta
cctatctcttaaatagtcattttcaatacaactttttctttttcatcacagccttaatgctccttagataataaccatt
atctaacaatgagatacctaattcttagcgtgcctgttaacccacacatcagggtctatgcttattaaaagcaggcaaa
aagcatgttttacggtaaagcccctgcgtttgctgggttgaactgataatcattatcactaacatgctgttatacgctg
aaagcgatgaagtgaggtaaatcgtactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaaga
gttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgt
ccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccc
cgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccgga
aggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagac
ggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgg
gttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaa
agacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaa
accgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtc
actccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttatatactagagcctcacccgtttgccaacacggacatttggtacgctgatgagcatgg
aaccggcgctggctgccgtttccgggatgattttcctcggagaaacactgacacccatacagctactggcgctcggcgc
tatcatcgccgcttcaatggggtctacgctgacagtacgcaaagagagcaaaataaaagaattagacattaattaaatt
tacatttctgcatggttatgcataaccatgcagaatttctcgctacttttcctctacaccgtctttatatatcgaatta
tgcaaaagcatatttattccgaaaattcctggcgagcagataaataagaattgttcttatcaatatatctaactcattg
aatctttattagttttgtttttcacgcttgttaccactattagtgtgataggaacagccagaatagcggaacacatagc
cggtgctatacttaatctcgttaattactgggacataacatcaagaggatatgaaatttactagagaaagaggagaaat
actagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacgg
ccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccacc
ggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgacc
acatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacga
cggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgac
ttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgaca
agcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccacta
ccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagc
aaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacg
agctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata