BBa_K363001 1 BBa_K363001 The consensus calcium dependent response element binding site for the Crz1 activator. 2010-10-24T11:00:00Z 2015-05-08T01:12:13Z This part was derived from calcium dependent response element (CDRE) binding sequence of the FSK2 gene. The consensus sequence for Crz1 binding as described by Yoshimoto et al (2002) was mutated at non conserved residues to generate this part. This UAS is the consensus sequence for Crz1 binding, and was used in characterization experiments. This part is a binding site for the Crz1 transcriptional activator. When placed upstream of a eukaryotic promoter sequence, this sequence results in the calcium dependant transcription of downstream coding sequences given that Crz1 and the upstream signal transducing phosphotase calcineurin are constitutively expressed. The signal cascade occurs when calcineurin binds to cytosolic calcium, activating its phosphotase activity. The active calcineurin dephosphorylates cytoplasmic Crz1, resulting in translocation of Crz1 to the nucleus where it binds to this part and promotes the assembly of general transcription factors at the promoter sequence. false false _472_ 0 6798 9 It's complicated true This part is NOT itself a promoter. It must be inserted upstream of a promoter to function. false Noah Young annotation2100719 1 Crz1 Binding Site range2100719 1 1 7 BBa_K363001_sequence 1 gaggctg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z