LOCUS       BBa_K364333             1670 bp    DNA     linear       14-May-2024
DEFINITION  TRE-Gal4-EcR-PolyA in PSB1A3 (expression vector)
ACCESSION   BBa_K364333
VERSION     BBa_K364333.1  
FEATURES             Location/Qualifiers
     misc_feature    1..249
                     /label=TRE
     promoter        255..315
                     /label=minimal P-CMV
     misc_feature    9..27
                     /label=1st tetO
     misc_feature    45..63
                     /label=2nd tetO
     misc_feature    80..98
                     /label=3rd tetO
     misc_feature    116..134
                     /label=4th tetO
     misc_feature    152..170
                     /label=5th tetO
     misc_feature    187..205
                     /label=6th tetO
     misc_feature    223..241
                     /label=7th tetO
     promoter        1..321
                     /label=BBa_K364304
     CDS             330..770
                     /label=BBa_K364319
     CDS             779..1471
                     /label=BBa_K364310
     terminator      1480..1670
                     /label=BBa_K364302
ORIGIN
        1 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
       61 agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
      121 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
      181 cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
      241 agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
      301 tgaaccgtca gatcgcctgg atactagagg ccaagctact gtcttctatc gaacaagcat
      361 gcgatatttg ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt
      421 gtctgaagaa caactgggag tgtcgctact ctcccaaaac caaaaggtct ccgctgacta
      481 gggcacatct gacagaagtg gaatcaaggc tagaaagact ggaacagcta tttctactga
      541 tttttcctcg agaagacctt gacatgattt tgaaaatgga ttctttacag gatataaaag
      601 cattgttaac aggattattt gtacaagata atgtgaataa agatgccgtc acagatagat
      661 tggcttcagt ggagactgat atgcctctaa cattgagaca gcatagaata agtgcgacat
      721 catcatcgga agagagtagt aacaaaggtc aaagacagtt gactgtatcg tactagagct
      781 ggccgtgatc tacaagctca tttggtatca ggacggctac gagcagccca gcgaagagga
      841 cctgcggcgg atcatgagcc agcccgacga gaacgagagc cagaccgacg tgtccttccg
      901 gcacatcacc gagatcacca tcctgaccgt gcagctcatc gtggaatttg ccaagggcct
      961 gcccgccttc accaagatcc cccaggaaga tcagatcacc ctgctgaagg cctgctccag
     1021 cgaagtgatg atgctgcgga tggccagaag atacgaccac agcagcgaca gcatcttctt
     1081 cgccaacaac agaagctaca cccgggacag ctacaagatg gctggcatgg ccgacaacat
     1141 cgaggacctg ctgcacttct gtcggcagat gttcagcatg aaggtggaca acgtggaata
     1201 cgccctgctg accgccatcg tgatcttcag cgacagaccc ggcctggaaa aggcccagct
     1261 cgtggaagcc atccagagct actacatcga caccctgcgg atctacatcc tgaaccggca
     1321 ctgcggcgac agcatgagcc tggtgttcta cgccaagctg ctgagcatcc tgacagagct
     1381 gcggaccctg ggcaaccaga acgccgagat gtgcttcagc ctgaagctga agaaccggaa
     1441 gctgcccaag ttcctggaag agatctggga ttactagagg gatcataatc agccatacca
     1501 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     1561 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     1621 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcct
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