BBa_K365005
1
linker
20 aa linker RFC-25 (fusion standard Freiburg)
2010-09-13T11:00:00Z
2015-05-08T01:12:15Z
DNA synthesis.
20 amino acids linker initially designed to link different ClpX subunits, according to the work of T.Baker et al.
false
false
_477_
0
6082
9
It's complicated
false
None.
false
STIEFEL Fabian & KALCHSCHMIDT Jens Sebastian
BBa_K365001
1
PIF-6
Phytochrome Interacting Factor 6 (aa 1-100)
2010-09-13T11:00:00Z
2015-05-08T01:12:15Z
The sequence of full lenght PIF-3 cDNA was a gift from Prf. Weber.
First hundred aminoacids of Phytochrome Interacting Factor 6 (PIF6) from A. thaliana, which physically interacts with Phytochrome B.
false
false
_477_
0
6082
9
In stock
true
We needed to delete one restriction site by directed mutagenesis.
false
RENAULT Renaud
annotation2079908
1
start
range2079908
1
1
3
BBa_K365015
1
BBa_K365015
PIF6-linker-GFP
2010-10-26T11:00:00Z
2015-05-08T01:12:15Z
Assembly RFC 25
PIF6-linker-GFP
false
false
_477_
0
6082
9
Not in stock
false
none
false
RENAULT Renaud
component2109540
1
BBa_B0105
component2109535
1
BBa_K365001
component2109538
1
BBa_K365005
component2109537
1
BBa_B0105
component2109542
1
BBa_K365009
annotation2109542
1
BBa_K365009
range2109542
1
373
1086
annotation2109535
1
BBa_K365001
range2109535
1
1
300
annotation2109540
1
BBa_B0105
range2109540
1
367
372
annotation2109537
1
BBa_B0105
range2109537
1
301
306
annotation2109538
1
BBa_K365005
range2109538
1
307
366
BBa_K365009
1
GFPsf
GFP superfolder RFC 25
2010-10-09T11:00:00Z
2015-05-08T01:12:15Z
We extracted the GFP superfolder sequence from the I746915 biobrick, using appropriate primers to add fusion prefix and suffix.
GFP superfolder sequence with RFC 25 fusion standard.
false
false
_477_
0
6082
9
It's complicated
false
We deleted the double stop codon to allow fusion assembly.
false
RENAULT Renaud
annotation2084613
1
start
range2084613
1
1
3
BBa_B0105
1
Scar 25
RFC 25 Scar Sequence
2009-10-14T11:00:00Z
2015-08-31T04:07:21Z
BBF RFC 25
This is the scar produced by assembly using RFC 25.
If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
false
false
_1_
0
25
397
Not in stock
false
Simple DNA sequence
false
Randy Rettberg
annotation2041621
1
Scar 25
range2041621
1
1
6
BBa_K365009_sequence
1
atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaa
BBa_K365001_sequence
1
atgatgttcttaccaaccgattattgttgcaggttaagcgatcaagagtatatggagcttgtgtttgagaatggccagattcttgcaaagggccaaagatccaacgtttctctgcataatcaacgtaccaaatcgatcatggatttgtatgaggcagagtataacgaggatttcatgaagagtatcatccatggtggtggtggtgccatcacaaatctcggggacacgcaggttgttccacaaagtcatgttgctgctgcccatgaaacaaacatgttggaaagcaataaacatgttgac
BBa_B0105_sequence
1
accggc
BBa_K365005_sequence
1
gcgagcggcgcgggcggcagcgaaggcggcggcagcgaaggcggcaccagcggcgcgacc
BBa_K365015_sequence
1
atgatgttcttaccaaccgattattgttgcaggttaagcgatcaagagtatatggagcttgtgtttgagaatggccagattcttgcaaagggccaaagatccaacgtttctctgcataatcaacgtaccaaatcgatcatggatttgtatgaggcagagtataacgaggatttcatgaagagtatcatccatggtggtggtggtgccatcacaaatctcggggacacgcaggttgttccacaaagtcatgttgctgctgcccatgaaacaaacatgttggaaagcaataaacatgttgacaccggcgcgagcggcgcgggcggcagcgaaggcggcggcagcgaaggcggcaccagcggcgcgaccaccggcatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z