BBa_K365005 1 linker 20 aa linker RFC-25 (fusion standard Freiburg) 2010-09-13T11:00:00Z 2015-05-08T01:12:15Z DNA synthesis. 20 amino acids linker initially designed to link different ClpX subunits, according to the work of T.Baker et al. false false _477_ 0 6082 9 It's complicated false None. false STIEFEL Fabian & KALCHSCHMIDT Jens Sebastian BBa_K365001 1 PIF-6 Phytochrome Interacting Factor 6 (aa 1-100) 2010-09-13T11:00:00Z 2015-05-08T01:12:15Z The sequence of full lenght PIF-3 cDNA was a gift from Prf. Weber. First hundred aminoacids of Phytochrome Interacting Factor 6 (PIF6) from A. thaliana, which physically interacts with Phytochrome B. false false _477_ 0 6082 9 In stock true We needed to delete one restriction site by directed mutagenesis. false RENAULT Renaud annotation2079908 1 start range2079908 1 1 3 BBa_K365015 1 BBa_K365015 PIF6-linker-GFP 2010-10-26T11:00:00Z 2015-05-08T01:12:15Z Assembly RFC 25 PIF6-linker-GFP false false _477_ 0 6082 9 Not in stock false none false RENAULT Renaud component2109540 1 BBa_B0105 component2109535 1 BBa_K365001 component2109538 1 BBa_K365005 component2109537 1 BBa_B0105 component2109542 1 BBa_K365009 annotation2109542 1 BBa_K365009 range2109542 1 373 1086 annotation2109535 1 BBa_K365001 range2109535 1 1 300 annotation2109540 1 BBa_B0105 range2109540 1 367 372 annotation2109537 1 BBa_B0105 range2109537 1 301 306 annotation2109538 1 BBa_K365005 range2109538 1 307 366 BBa_K365009 1 GFPsf GFP superfolder RFC 25 2010-10-09T11:00:00Z 2015-05-08T01:12:15Z We extracted the GFP superfolder sequence from the I746915 biobrick, using appropriate primers to add fusion prefix and suffix. GFP superfolder sequence with RFC 25 fusion standard. false false _477_ 0 6082 9 It's complicated false We deleted the double stop codon to allow fusion assembly. false RENAULT Renaud annotation2084613 1 start range2084613 1 1 3 BBa_B0105 1 Scar 25 RFC 25 Scar Sequence 2009-10-14T11:00:00Z 2015-08-31T04:07:21Z BBF RFC 25 This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence. false false _1_ 0 25 397 Not in stock false Simple DNA sequence false Randy Rettberg annotation2041621 1 Scar 25 range2041621 1 1 6 BBa_K365009_sequence 1 atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaa BBa_K365001_sequence 1 atgatgttcttaccaaccgattattgttgcaggttaagcgatcaagagtatatggagcttgtgtttgagaatggccagattcttgcaaagggccaaagatccaacgtttctctgcataatcaacgtaccaaatcgatcatggatttgtatgaggcagagtataacgaggatttcatgaagagtatcatccatggtggtggtggtgccatcacaaatctcggggacacgcaggttgttccacaaagtcatgttgctgctgcccatgaaacaaacatgttggaaagcaataaacatgttgac BBa_B0105_sequence 1 accggc BBa_K365005_sequence 1 gcgagcggcgcgggcggcagcgaaggcggcggcagcgaaggcggcaccagcggcgcgacc BBa_K365015_sequence 1 atgatgttcttaccaaccgattattgttgcaggttaagcgatcaagagtatatggagcttgtgtttgagaatggccagattcttgcaaagggccaaagatccaacgtttctctgcataatcaacgtaccaaatcgatcatggatttgtatgaggcagagtataacgaggatttcatgaagagtatcatccatggtggtggtggtgccatcacaaatctcggggacacgcaggttgttccacaaagtcatgttgctgctgcccatgaaacaaacatgttggaaagcaataaacatgttgacaccggcgcgagcggcgcgggcggcagcgaaggcggcggcagcgaaggcggcaccagcggcgcgaccaccggcatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z