LOCUS BBa_K367006 2292 bp DNA linear 22-Nov-2024 DEFINITION fecA-prhA fusion coding sequence ACCESSION BBa_K367006 VERSION BBa_K367006.1 FEATURES Location/Qualifiers CDS 1..2286 /label=Fused fecA-prhA coding sequence misc_feature 1..3 /label=fecA start codon misc_feature 1..99 /label=Encodes FecA signal peptide CDS 100..276 /label=Encodes FecA N-terminal extension domain misc_feature 265..276 /label=Encodes conserved GSGL motif, common to FecA and PrhA CDS 265..2286 /label=Encodes PrhA C-terminus, starting at amino acid 97 misc_feature 2287..2292 /label=Standard biobrick double stop codon ORIGIN 1 atgacacctc tgcgcgtttt tcgtaaaacc accccactgg ttaacactat ccgtctgagc 61 ctgctgcctc tggctggtct gtctttttct gcctttgctg cccaggtcaa tattgctccg 121 ggttcgctgg ataaagcact gaatcagtat gccgcccatt ctgggttcac tctgagtgtg 181 gatgcttctc tgacacgtgg taaacaaagc aacggtctgc atggcgatta tgatgtggaa 241 tctggactgc aacaactgct ggacggttca ggtctgacgc tggcatctct gccggatggt 301 acactgactg ttgtggcgtc tgatcaagct gaagctccgc cgaatgttgg aacactggct 361 gctaccgaag ttcatggtga ggctggggat ggattcgccg tgttttctac cggtgccatt 421 tctcgtgttg agatggccgt tagcaatgtc cctcatgctg tgtctatgct gtctggtggg 481 ctgctggcta gtcgtcaatc tcctaccctg tccgaagctg tttcacaagc tggtgttgtg 541 cctgttctgg tcgatcaagc cgctcctcct cagtatgcta ttcgtgggtt catgaccgat 601 acaatctcgg ttgatggtct gcctgaccgc ctggctagta tgcgtcctat ggaagctctg 661 gatgaaattg ccgtaatcaa agggcctaat gccgatgtcg ctggcgttac tatggctggc 721 gggacgatta atgcctcgct gaaagctcca ggtgctgctc ctatgcgttc tattgccgtg 781 gaaaccggct ctcatgctga acgtaaagtg atcgccgatc tgggaggtgc tattggtaca 841 aacgggctga tctatcgtgt agtaggggta caggatgcta gtgccaattc tgatgctggt 901 cataccggtc gtcgtacctc ttatggtcac ggtgctctgg gatggagtga tgctaatacc 961 gaggctatca ttggcgtaga gacactggca agccgtcagc ctgttcaacc ttccaccttt 1021 gtgctgaatg gtggcgttgt tcgcctgcct tcactgtcac cgctggggaa tgctgatgat 1081 gatgtccaag gtcgtgccga acgtttctat tatacgctgt cccactctct gaccgatgac 1141 tgggctgttc attctcgtgg ctcctatgaa aatctgcgcc tgcaatctgc tcagtggcac 1201 ctgatgccat ctgccgacgg tagtgctcca atgcctaccg ttggtatggg ttatcgttcc 1261 gacaaccgtg tgtgggcact gtctagtgat ctgactggga ctattcgtca aggtccgctg 1321 acacattctc tggtcgttgg ttgggatgag atccaggatc gtctggattt ccattatccg 1381 ggtgctacag tactgggtca acagaaccct tatgtgccga ctccactgcc aagtgccgag 1441 ttcgttccag gactgagtct gtcacaaacc gttcgtcagt cggttttccg cctgcgtgat 1501 cgtattgcca ttggtgaacg ctgggaactg agtgcctcaa ttcgtgctaa tgactatgtg 1561 gccaaactgc ctcatgctcg tctgcaaggt ctggcctgga ctcctgcttt tggcgtggtg 1621 tataaactgg atgcccgtac aagttggttt gccgattatt ctcatgggtt tcagattaac 1681 accgggtatt tctataacgg tgctacgatg cagcctgagc gtagtcagca agtcgaaacg 1741 ggtctgcgtt gggaagatcg tcagcgtaaa ctgacagcac agctggctct gtatcacatt 1801 gatgctaatc acgtgggtgt tggcgatact gcccatccag gctattatac cgaaatcacc 1861 gaacagagtt ccgatggtat cgaactgtct ctgcaaggct ctattgctcg tggttgggaa 1921 acttctctgt ggctgagtgg tgctcatgtt aaacgccgtc tgccgggtaa tccttctttt 1981 accgctccag gactgggagg ggccatgtgg acaacctata cggtccagtc tggtacactg 2041 gctggtgctg gtgctggtct gggtgtgact gctcaacgtt ggattccgtg ttatgatacc 2101 gccccttttc gtgtcccggg tttcgtacaa ctggacggct cactgttttg gcgctctaaa 2161 caatggcgta tcgacctgat tgcccgtaac ctgttcaacg tccgtgctta tggtaacact 2221 gtgtcctcat cgttcgtgcc tatcctgcca gggcgtaccc tgacactgcg tctggctcgt 2281 aacttttaat aa //