BBa_K367006 1 fecA-prhA fecA-prhA fusion coding sequence 2010-06-29T11:00:00Z 2015-05-08T01:12:15Z fecA sequence comes from Escherichia coli str. K-12 substr. MG1655, complete genome. GenBank: U00096.2 prhA sequence comes from Ralstonia solanacearum GMI1000 plasmid pGMI1000MP, complete sequence. NCBI Reference Sequence: NC_003296.1 Artificial coding sequence spanning the first 92 codons of fecA, encoding the signal peptide (aa. 1-33), the proposed Ton-box (aa. 54-63), fused to the distal end of the prhA coding sequence at the conserved GSGL motif (aa. 89-92). false false _492_ 0 6157 9 It's complicated true No edits other than the fusion junction, and the introduction of the standard biobrick stop codons TAATAA false Fernando Govantes annotation2072057 1 Standard biobrick double stop codon range2072057 1 2287 2292 annotation2072053 1 Encodes FecA signal peptide range2072053 1 1 99 annotation2072055 1 Encodes conserved GSGL motif, common to FecA and PrhA range2072055 1 265 276 annotation2072056 1 Encodes PrhA C-terminus, starting at amino acid 97 range2072056 1 265 2286 annotation2072051 1 Fused fecA-prhA coding sequence range2072051 1 1 2286 annotation2072052 1 fecA start codon range2072052 1 1 3 annotation2072054 1 Encodes FecA N-terminal extension domain range2072054 1 100 276 BBa_K367006_sequence 1 atgacacctctgcgcgtttttcgtaaaaccaccccactggttaacactatccgtctgagcctgctgcctctggctggtctgtctttttctgcctttgctgcccaggtcaatattgctccgggttcgctggataaagcactgaatcagtatgccgcccattctgggttcactctgagtgtggatgcttctctgacacgtggtaaacaaagcaacggtctgcatggcgattatgatgtggaatctggactgcaacaactgctggacggttcaggtctgacgctggcatctctgccggatggtacactgactgttgtggcgtctgatcaagctgaagctccgccgaatgttggaacactggctgctaccgaagttcatggtgaggctggggatggattcgccgtgttttctaccggtgccatttctcgtgttgagatggccgttagcaatgtccctcatgctgtgtctatgctgtctggtgggctgctggctagtcgtcaatctcctaccctgtccgaagctgtttcacaagctggtgttgtgcctgttctggtcgatcaagccgctcctcctcagtatgctattcgtgggttcatgaccgatacaatctcggttgatggtctgcctgaccgcctggctagtatgcgtcctatggaagctctggatgaaattgccgtaatcaaagggcctaatgccgatgtcgctggcgttactatggctggcgggacgattaatgcctcgctgaaagctccaggtgctgctcctatgcgttctattgccgtggaaaccggctctcatgctgaacgtaaagtgatcgccgatctgggaggtgctattggtacaaacgggctgatctatcgtgtagtaggggtacaggatgctagtgccaattctgatgctggtcataccggtcgtcgtacctcttatggtcacggtgctctgggatggagtgatgctaataccgaggctatcattggcgtagagacactggcaagccgtcagcctgttcaaccttccacctttgtgctgaatggtggcgttgttcgcctgccttcactgtcaccgctggggaatgctgatgatgatgtccaaggtcgtgccgaacgtttctattatacgctgtcccactctctgaccgatgactgggctgttcattctcgtggctcctatgaaaatctgcgcctgcaatctgctcagtggcacctgatgccatctgccgacggtagtgctccaatgcctaccgttggtatgggttatcgttccgacaaccgtgtgtgggcactgtctagtgatctgactgggactattcgtcaaggtccgctgacacattctctggtcgttggttgggatgagatccaggatcgtctggatttccattatccgggtgctacagtactgggtcaacagaacccttatgtgccgactccactgccaagtgccgagttcgttccaggactgagtctgtcacaaaccgttcgtcagtcggttttccgcctgcgtgatcgtattgccattggtgaacgctgggaactgagtgcctcaattcgtgctaatgactatgtggccaaactgcctcatgctcgtctgcaaggtctggcctggactcctgcttttggcgtggtgtataaactggatgcccgtacaagttggtttgccgattattctcatgggtttcagattaacaccgggtatttctataacggtgctacgatgcagcctgagcgtagtcagcaagtcgaaacgggtctgcgttgggaagatcgtcagcgtaaactgacagcacagctggctctgtatcacattgatgctaatcacgtgggtgttggcgatactgcccatccaggctattataccgaaatcaccgaacagagttccgatggtatcgaactgtctctgcaaggctctattgctcgtggttgggaaacttctctgtggctgagtggtgctcatgttaaacgccgtctgccgggtaatccttcttttaccgctccaggactgggaggggccatgtggacaacctatacggtccagtctggtacactggctggtgctggtgctggtctgggtgtgactgctcaacgttggattccgtgttatgataccgccccttttcgtgtcccgggtttcgtacaactggacggctcactgttttggcgctctaaacaatggcgtatcgacctgattgcccgtaacctgttcaacgtccgtgcttatggtaacactgtgtcctcatcgttcgtgcctatcctgccagggcgtaccctgacactgcgtctggctcgtaacttttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z