BBa_K368024 1 BBa_K368024 tet-on Promoter (prev TRE) + attB (bacterial attachment site) 2010-10-23T11:00:00Z 2015-05-08T01:12:15Z from a plasmid for eukaryots: inducible promoter (tet-on; = prev TRE); inducible by doxycycline + bacterial attachment site (attB); PhiC31 (or PhiC31o) will integrate genes combined with attP-site into attB- site false false _483_ 0 6378 9 Not in stock false integrate the eGFP sequence where attB site is, so that eGFp will be translated after induction with docycycline false Jara Radeck BBa_K368002 1 BBa_K368002 Human Bak + SV40 Polyadenylation site 2010-10-23T11:00:00Z 2015-05-08T01:12:15Z Human cDNA Eykaryots: Human Bak, a mitochondrial membrane protein which leads to apoptosis if overexpressed + SV40PA (Polyadenylation site); for translation in eukaryots no promoter! false false _483_ 0 6378 9 Not in stock false induces apoptosis if a cell did not incorporate the gene of interest -> "Jump-Or-Die" system; "Cut'N'survive" system false Jara Radeck BBa_K368003 1 BBa_K368003 tet-on Promoter (prev TRE) + attB + Human Bak + SV40PA 2010-10-23T11:00:00Z 2015-05-08T01:12:15Z see: BBa_K368024, BBa_K368002 eukaryots: tet-on Promoter (prev TRE): inducible promoter by doxycycline attB: bacterial attachment site -> PhiC31 or PhiC31o Human Bak: mitochondrial membrane protein which induces apoptosis if overexpressed SV40PA: Poyladenylation signal, necessary for tranlation in eukaryots false false _483_ 0 6378 9 Not in stock false -> "Jump-Or-Die" system false Jara Radeck component2094896 1 BBa_K368024 component2094897 1 BBa_K368002 annotation2094897 1 BBa_K368002 range2094897 1 494 1337 annotation2094896 1 BBa_K368024 range2094896 1 1 487 BBa_K368024_sequence 1 ctcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagctcggtacccgggtcgagtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccgcggtactagagggtgccagggcgtgcccttgggctccccgggcgcg BBa_K368003_sequence 1 ctcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagctcggtacccgggtcgagtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccgcggtactagagggtgccagggcgtgcccttgggctccccgggcgcgtactagatggcttcggggcaaggcccaggtcctcccaggcaggagtgcggagagcctgccctgccctctgcttctgaggagcaggtagcccaggacacagaggaggttttccgcagctacgttttttaccgccatcagcaggaacaggaggctgaaggggtggctgcccctgccgacccagagatggtcaccttacctctgcaacctagcagcaccatggggcaggtgggacggcagctcgccatcatcggggacgacatcaaccgacgctatgactcagagttccagaccatgttgcagcacctacagcccacggcagagaatgcctatgagtacttcaccaagattgccaccagcctgtttgagagtggcatcaattggggccgtgtggtggctcttctgggcttcggctaccgtctggccctacacgtctaccagcatggcctgactggcttcctaggccaggtgacccgcttcgtggtcgacttcatgctgcatcactgcattgcccggtggattgcacagaggggtggctgggtggcagccctgaacttgggcaatggtcccatcctgaacgtgctggtggttctgggtgtggttctgttgggccagtttgtggtacgaagattcttcaaatcatgatactagagctagaggatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcctcgag BBa_K368002_sequence 1 atggcttcggggcaaggcccaggtcctcccaggcaggagtgcggagagcctgccctgccctctgcttctgaggagcaggtagcccaggacacagaggaggttttccgcagctacgttttttaccgccatcagcaggaacaggaggctgaaggggtggctgcccctgccgacccagagatggtcaccttacctctgcaacctagcagcaccatggggcaggtgggacggcagctcgccatcatcggggacgacatcaaccgacgctatgactcagagttccagaccatgttgcagcacctacagcccacggcagagaatgcctatgagtacttcaccaagattgccaccagcctgtttgagagtggcatcaattggggccgtgtggtggctcttctgggcttcggctaccgtctggccctacacgtctaccagcatggcctgactggcttcctaggccaggtgacccgcttcgtggtcgacttcatgctgcatcactgcattgcccggtggattgcacagaggggtggctgggtggcagccctgaacttgggcaatggtcccatcctgaacgtgctggtggttctgggtgtggttctgttgggccagtttgtggtacgaagattcttcaaatcatgatactagagctagaggatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcctcgag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z