BBa_K368019 1 BBa_K368019 TEV-Protease+p14*+TEV-recognition site 2010-09-23T11:00:00Z 2015-05-08T01:12:15Z (Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009)) 1. and 2. were in a plasmid, 3. was added by a primer 1.TEV-Protease 2.Protein p14* (interacting with SF3b155, BBa_K368016) 3.TEV-Protease-recognition site false false _483_ 0 6378 9 It's complicated false It??s part of BBa_K..... false Jara Radeck BBa_K368001 1 BBa_K368001 minimal CMV Promoter with TRE (tet responsible element) 2010-09-20T11:00:00Z 2015-05-08T01:12:15Z pRev TRE tet-on-promoter CMV promoter induceable by Doxycycline. Only works in special Tet-On cell lines (stable transfected with pTet-On-Advanced Vector by clontech)! Also works in special Tet-Off cell lines (stable transfected with pTet-Off-Advanced Vector by clontech), but then the promoter will be inactivated by Doxycycline. false false _483_ 0 6378 9 Not in stock false We used Primers with Biobrick digestion sites and ligated with pSB1C3 false Jara Radeck BBa_K368009 1 BBa_K368009 tet-on Promoter+TEV recogn.site+N-degron+SF3b155+Human Bak+SV40PA 2010-10-23T11:00:00Z 2015-05-08T01:12:15Z (Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009)) eukaryots: tet-on Promoter: doxycycline inducible CMV promoter (special cell line needed!) TEV recogn.site: specific restriction site for TEV protease N-degron: degradation signal for proteins if N-terminus is free SF3b155: Splicing factor 3b, size: 155kDa, interacting with p14* Human Bak: mitochondrial membrane protein, causing apoptosis if overexpressed SV40PA: polyadenylation signal, needed for translation in eukaryots false false _483_ 0 6378 9 Not in stock false -> "Cut'N'survive" system false Jara Radeck component2094905 1 BBa_K368002 component2094904 1 BBa_K368007 annotation2094904 1 BBa_K368007 range2094904 1 1 1635 annotation2094905 1 BBa_K368002 range2094905 1 1642 2485 BBa_K368007 1 BBa_K368007 tet-on Promoter (prev TRE) + TEV recogn. site+ N-degron+SF3b155 2010-10-23T11:00:00Z 2015-05-08T01:12:15Z (Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009)) eukaryots: tet-on Promoter (prev TRE): inducible CMV promoter, inducible by doxycycline TEV recogn. site: specific recognition site for TEV protease N-degron: Protein degradation signal, if N-terminus is free SF3b155: Splicing factor 3b, size: 155kDa; interacting with p14* false false _483_ 0 6378 9 Not in stock false -> "Cut'N'Survive" system false Jara Radeck component2094900 1 BBa_K368001 component2094901 1 BBa_K368019 annotation2094901 1 BBa_K368019 range2094901 1 451 1635 annotation2094900 1 BBa_K368001 range2094900 1 1 444 BBa_K368002 1 BBa_K368002 Human Bak + SV40 Polyadenylation site 2010-10-23T11:00:00Z 2015-05-08T01:12:15Z Human cDNA Eykaryots: Human Bak, a mitochondrial membrane protein which leads to apoptosis if overexpressed + SV40PA (Polyadenylation site); for translation in eukaryots no promoter! false false _483_ 0 6378 9 Not in stock false induces apoptosis if a cell did not incorporate the gene of interest -> "Jump-Or-Die" system; "Cut'N'survive" system false Jara Radeck BBa_K368009_sequence 1 ctcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagctcggtacccgggtcgagtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccgcggtactagatggcgatgcaagcggccaagagggcgaacattcgacttccacctgaagtaaatcggatattgtatataagaaatttgccatacaaaatcacagctgaagaaatgtatgatatatttgggaaatatggacctattcgtcaaatcagagtggggaacacacctgaaactagaggaacagcttatgtggtctatgaggacatctttgatgccaagaatgcatgtgatcacctatcgggattcaatgtttgtaacagataccttgtggttttgtactataatgccaacagggcatttcagaagatggacacaaagaagaaggaggaacagttgaagcttctcaaggagaaatatggcatcaacacatatcctcccaagatgaaacatcaccatcaccatcaccccatgagcggcctggtgccgcgcggcagcgccatgggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttcgtcgcaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaaggacaccacgactttgcaacaacacctcattgatgggagggacatgatgattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaagctaagagcatgtctagcatggtgtcagacacgagttgcacattcccttcatctgatggtatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaacgctgactcagtattgtgggggggccataaagttttcatggttaaacctgaagaaccttttcagccagttaaggaagcgactcaactcatgaatgaattggtgtactcgcaaggggaaaacttgtacttccaattctactagatggcttcggggcaaggcccaggtcctcccaggcaggagtgcggagagcctgccctgccctctgcttctgaggagcaggtagcccaggacacagaggaggttttccgcagctacgttttttaccgccatcagcaggaacaggaggctgaaggggtggctgcccctgccgacccagagatggtcaccttacctctgcaacctagcagcaccatggggcaggtgggacggcagctcgccatcatcggggacgacatcaaccgacgctatgactcagagttccagaccatgttgcagcacctacagcccacggcagagaatgcctatgagtacttcaccaagattgccaccagcctgtttgagagtggcatcaattggggccgtgtggtggctcttctgggcttcggctaccgtctggccctacacgtctaccagcatggcctgactggcttcctaggccaggtgacccgcttcgtggtcgacttcatgctgcatcactgcattgcccggtggattgcacagaggggtggctgggtggcagccctgaacttgggcaatggtcccatcctgaacgtgctggtggttctgggtgtggttctgttgggccagtttgtggtacgaagattcttcaaatcatgatactagagctagaggatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcctcgag BBa_K368001_sequence 1 ctcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagctcggtacccgggtcgagtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccgcgg BBa_K368019_sequence 1 atggcgatgcaagcggccaagagggcgaacattcgacttccacctgaagtaaatcggatattgtatataagaaatttgccatacaaaatcacagctgaagaaatgtatgatatatttgggaaatatggacctattcgtcaaatcagagtggggaacacacctgaaactagaggaacagcttatgtggtctatgaggacatctttgatgccaagaatgcatgtgatcacctatcgggattcaatgtttgtaacagataccttgtggttttgtactataatgccaacagggcatttcagaagatggacacaaagaagaaggaggaacagttgaagcttctcaaggagaaatatggcatcaacacatatcctcccaagatgaaacatcaccatcaccatcaccccatgagcggcctggtgccgcgcggcagcgccatgggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttcgtcgcaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaaggacaccacgactttgcaacaacacctcattgatgggagggacatgatgattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaagctaagagcatgtctagcatggtgtcagacacgagttgcacattcccttcatctgatggtatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaacgctgactcagtattgtgggggggccataaagttttcatggttaaacctgaagaaccttttcagccagttaaggaagcgactcaactcatgaatgaattggtgtactcgcaaggggaaaacttgtacttccaattc BBa_K368002_sequence 1 atggcttcggggcaaggcccaggtcctcccaggcaggagtgcggagagcctgccctgccctctgcttctgaggagcaggtagcccaggacacagaggaggttttccgcagctacgttttttaccgccatcagcaggaacaggaggctgaaggggtggctgcccctgccgacccagagatggtcaccttacctctgcaacctagcagcaccatggggcaggtgggacggcagctcgccatcatcggggacgacatcaaccgacgctatgactcagagttccagaccatgttgcagcacctacagcccacggcagagaatgcctatgagtacttcaccaagattgccaccagcctgtttgagagtggcatcaattggggccgtgtggtggctcttctgggcttcggctaccgtctggccctacacgtctaccagcatggcctgactggcttcctaggccaggtgacccgcttcgtggtcgacttcatgctgcatcactgcattgcccggtggattgcacagaggggtggctgggtggcagccctgaacttgggcaatggtcccatcctgaacgtgctggtggttctgggtgtggttctgttgggccagtttgtggtacgaagattcttcaaatcatgatactagagctagaggatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcctcgag BBa_K368007_sequence 1 ctcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagctcggtacccgggtcgagtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccgcggtactagatggcgatgcaagcggccaagagggcgaacattcgacttccacctgaagtaaatcggatattgtatataagaaatttgccatacaaaatcacagctgaagaaatgtatgatatatttgggaaatatggacctattcgtcaaatcagagtggggaacacacctgaaactagaggaacagcttatgtggtctatgaggacatctttgatgccaagaatgcatgtgatcacctatcgggattcaatgtttgtaacagataccttgtggttttgtactataatgccaacagggcatttcagaagatggacacaaagaagaaggaggaacagttgaagcttctcaaggagaaatatggcatcaacacatatcctcccaagatgaaacatcaccatcaccatcaccccatgagcggcctggtgccgcgcggcagcgccatgggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttcgtcgcaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaaggacaccacgactttgcaacaacacctcattgatgggagggacatgatgattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaagctaagagcatgtctagcatggtgtcagacacgagttgcacattcccttcatctgatggtatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaacgctgactcagtattgtgggggggccataaagttttcatggttaaacctgaagaaccttttcagccagttaaggaagcgactcaactcatgaatgaattggtgtactcgcaaggggaaaacttgtacttccaattc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z