BBa_K368019
1
BBa_K368019
TEV-Protease+p14*+TEV-recognition site
2010-09-23T11:00:00Z
2015-05-08T01:12:15Z
(Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009))
1. and 2. were in a plasmid, 3. was added by a primer
1.TEV-Protease
2.Protein p14* (interacting with SF3b155, BBa_K368016)
3.TEV-Protease-recognition site
false
false
_483_
0
6378
9
It's complicated
false
It??s part of BBa_K.....
false
Jara Radeck
BBa_K368001
1
BBa_K368001
minimal CMV Promoter with TRE (tet responsible element)
2010-09-20T11:00:00Z
2015-05-08T01:12:15Z
pRev TRE tet-on-promoter
CMV promoter induceable by Doxycycline. Only works in special Tet-On cell lines (stable transfected with pTet-On-Advanced Vector by clontech)!
Also works in special Tet-Off cell lines (stable transfected with pTet-Off-Advanced Vector by clontech), but then the promoter will be inactivated by Doxycycline.
false
false
_483_
0
6378
9
Not in stock
false
We used Primers with Biobrick digestion sites and ligated with pSB1C3
false
Jara Radeck
BBa_K368009
1
BBa_K368009
tet-on Promoter+TEV recogn.site+N-degron+SF3b155+Human Bak+SV40PA
2010-10-23T11:00:00Z
2015-05-08T01:12:15Z
(Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009))
eukaryots:
tet-on Promoter: doxycycline inducible CMV promoter (special cell line needed!)
TEV recogn.site: specific restriction site for TEV protease
N-degron: degradation signal for proteins if N-terminus is free
SF3b155: Splicing factor 3b, size: 155kDa, interacting with p14*
Human Bak: mitochondrial membrane protein, causing apoptosis if overexpressed
SV40PA: polyadenylation signal, needed for translation in eukaryots
false
false
_483_
0
6378
9
Not in stock
false
-> "Cut'N'survive" system
false
Jara Radeck
component2094904
1
BBa_K368007
component2094905
1
BBa_K368002
annotation2094904
1
BBa_K368007
range2094904
1
1
1635
annotation2094905
1
BBa_K368002
range2094905
1
1642
2485
BBa_K368007
1
BBa_K368007
tet-on Promoter (prev TRE) + TEV recogn. site+ N-degron+SF3b155
2010-10-23T11:00:00Z
2015-05-08T01:12:15Z
(Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009))
eukaryots:
tet-on Promoter (prev TRE): inducible CMV promoter, inducible by doxycycline
TEV recogn. site: specific recognition site for TEV protease
N-degron: Protein degradation signal, if N-terminus is free
SF3b155: Splicing factor 3b, size: 155kDa; interacting with p14*
false
false
_483_
0
6378
9
Not in stock
false
-> "Cut'N'Survive" system
false
Jara Radeck
component2094900
1
BBa_K368001
component2094901
1
BBa_K368019
annotation2094901
1
BBa_K368019
range2094901
1
451
1635
annotation2094900
1
BBa_K368001
range2094900
1
1
444
BBa_K368002
1
BBa_K368002
Human Bak + SV40 Polyadenylation site
2010-10-23T11:00:00Z
2015-05-08T01:12:15Z
Human cDNA
Eykaryots:
Human Bak, a mitochondrial membrane protein which leads to apoptosis if overexpressed
+ SV40PA (Polyadenylation site); for translation in eukaryots
no promoter!
false
false
_483_
0
6378
9
Not in stock
false
induces apoptosis if a cell did not incorporate the gene of interest -> "Jump-Or-Die" system; "Cut'N'survive" system
false
Jara Radeck
BBa_K368009_sequence
1
ctcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagctcggtacccgggtcgagtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccgcggtactagatggcgatgcaagcggccaagagggcgaacattcgacttccacctgaagtaaatcggatattgtatataagaaatttgccatacaaaatcacagctgaagaaatgtatgatatatttgggaaatatggacctattcgtcaaatcagagtggggaacacacctgaaactagaggaacagcttatgtggtctatgaggacatctttgatgccaagaatgcatgtgatcacctatcgggattcaatgtttgtaacagataccttgtggttttgtactataatgccaacagggcatttcagaagatggacacaaagaagaaggaggaacagttgaagcttctcaaggagaaatatggcatcaacacatatcctcccaagatgaaacatcaccatcaccatcaccccatgagcggcctggtgccgcgcggcagcgccatgggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttcgtcgcaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaaggacaccacgactttgcaacaacacctcattgatgggagggacatgatgattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaagctaagagcatgtctagcatggtgtcagacacgagttgcacattcccttcatctgatggtatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaacgctgactcagtattgtgggggggccataaagttttcatggttaaacctgaagaaccttttcagccagttaaggaagcgactcaactcatgaatgaattggtgtactcgcaaggggaaaacttgtacttccaattctactagatggcttcggggcaaggcccaggtcctcccaggcaggagtgcggagagcctgccctgccctctgcttctgaggagcaggtagcccaggacacagaggaggttttccgcagctacgttttttaccgccatcagcaggaacaggaggctgaaggggtggctgcccctgccgacccagagatggtcaccttacctctgcaacctagcagcaccatggggcaggtgggacggcagctcgccatcatcggggacgacatcaaccgacgctatgactcagagttccagaccatgttgcagcacctacagcccacggcagagaatgcctatgagtacttcaccaagattgccaccagcctgtttgagagtggcatcaattggggccgtgtggtggctcttctgggcttcggctaccgtctggccctacacgtctaccagcatggcctgactggcttcctaggccaggtgacccgcttcgtggtcgacttcatgctgcatcactgcattgcccggtggattgcacagaggggtggctgggtggcagccctgaacttgggcaatggtcccatcctgaacgtgctggtggttctgggtgtggttctgttgggccagtttgtggtacgaagattcttcaaatcatgatactagagctagaggatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcctcgag
BBa_K368001_sequence
1
ctcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagctcggtacccgggtcgagtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccgcgg
BBa_K368019_sequence
1
atggcgatgcaagcggccaagagggcgaacattcgacttccacctgaagtaaatcggatattgtatataagaaatttgccatacaaaatcacagctgaagaaatgtatgatatatttgggaaatatggacctattcgtcaaatcagagtggggaacacacctgaaactagaggaacagcttatgtggtctatgaggacatctttgatgccaagaatgcatgtgatcacctatcgggattcaatgtttgtaacagataccttgtggttttgtactataatgccaacagggcatttcagaagatggacacaaagaagaaggaggaacagttgaagcttctcaaggagaaatatggcatcaacacatatcctcccaagatgaaacatcaccatcaccatcaccccatgagcggcctggtgccgcgcggcagcgccatgggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttcgtcgcaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaaggacaccacgactttgcaacaacacctcattgatgggagggacatgatgattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaagctaagagcatgtctagcatggtgtcagacacgagttgcacattcccttcatctgatggtatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaacgctgactcagtattgtgggggggccataaagttttcatggttaaacctgaagaaccttttcagccagttaaggaagcgactcaactcatgaatgaattggtgtactcgcaaggggaaaacttgtacttccaattc
BBa_K368002_sequence
1
atggcttcggggcaaggcccaggtcctcccaggcaggagtgcggagagcctgccctgccctctgcttctgaggagcaggtagcccaggacacagaggaggttttccgcagctacgttttttaccgccatcagcaggaacaggaggctgaaggggtggctgcccctgccgacccagagatggtcaccttacctctgcaacctagcagcaccatggggcaggtgggacggcagctcgccatcatcggggacgacatcaaccgacgctatgactcagagttccagaccatgttgcagcacctacagcccacggcagagaatgcctatgagtacttcaccaagattgccaccagcctgtttgagagtggcatcaattggggccgtgtggtggctcttctgggcttcggctaccgtctggccctacacgtctaccagcatggcctgactggcttcctaggccaggtgacccgcttcgtggtcgacttcatgctgcatcactgcattgcccggtggattgcacagaggggtggctgggtggcagccctgaacttgggcaatggtcccatcctgaacgtgctggtggttctgggtgtggttctgttgggccagtttgtggtacgaagattcttcaaatcatgatactagagctagaggatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcctcgag
BBa_K368007_sequence
1
ctcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagctcggtacccgggtcgagtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccgcggtactagatggcgatgcaagcggccaagagggcgaacattcgacttccacctgaagtaaatcggatattgtatataagaaatttgccatacaaaatcacagctgaagaaatgtatgatatatttgggaaatatggacctattcgtcaaatcagagtggggaacacacctgaaactagaggaacagcttatgtggtctatgaggacatctttgatgccaagaatgcatgtgatcacctatcgggattcaatgtttgtaacagataccttgtggttttgtactataatgccaacagggcatttcagaagatggacacaaagaagaaggaggaacagttgaagcttctcaaggagaaatatggcatcaacacatatcctcccaagatgaaacatcaccatcaccatcaccccatgagcggcctggtgccgcgcggcagcgccatgggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttcgtcgcaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaaggacaccacgactttgcaacaacacctcattgatgggagggacatgatgattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaagctaagagcatgtctagcatggtgtcagacacgagttgcacattcccttcatctgatggtatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaacgctgactcagtattgtgggggggccataaagttttcatggttaaacctgaagaaccttttcagccagttaaggaagcgactcaactcatgaatgaattggtgtactcgcaaggggaaaacttgtacttccaattc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z