BBa_K368011 1 BBa_K368011 eGFP + SV40 Polyadenylation site 2010-10-19T11:00:00Z 2015-05-08T01:12:15Z eGFP is from BBa_I714891 SV40PA is from BBa_K368018 This vector contains a eucaryotic codon optimized GFP + SV40 Polyadenylation site for eucaryotic expression. Without promoter! false false _483_ 0 6378 9 It's complicated false add CMV+p14 (interacting with SF3b155)+TEV recognition site -> "Cut'N'Survive System" false Jara Radeck BBa_K368019 1 BBa_K368019 TEV-Protease+p14*+TEV-recognition site 2010-09-23T11:00:00Z 2015-05-08T01:12:15Z (Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009)) 1. and 2. were in a plasmid, 3. was added by a primer 1.TEV-Protease 2.Protein p14* (interacting with SF3b155, BBa_K368016) 3.TEV-Protease-recognition site false false _483_ 0 6378 9 It's complicated false It??s part of BBa_K..... false Jara Radeck BBa_K368010 1 BBa_K368010 CMV-Promoter + TEV-Protease+p14*+TEV recogn. site 2010-10-23T11:00:00Z 2015-05-08T01:12:15Z (Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009)) CMV promoter from registry seems to be wrong.... eukaryots: CMV-Promoter: konstitutive promoter from cucumber mosaic virus TEV-Protease: Protesase which cuts at the end of specific recognition sites p14*: Protein interacting with SF3b155 (Splicing factor 3b, size: 155kDa) TEV recogn. site: recognition site for TEV-protease false false _483_ 0 6378 9 Not in stock false -> "Cut'N'Survive" system false Jara Radeck component2094909 1 BBa_K368019 component2094908 1 BBa_I714891 annotation2094909 1 BBa_K368019 range2094909 1 743 1927 annotation2094908 1 BBa_I714891 range2094908 1 1 736 BBa_K368012 1 BBa_K368012 CMV-promoter+TEV-protease+p14*+TEV recogn. site+eGFP+SV40PA 2010-10-23T11:00:00Z 2015-05-08T01:12:15Z biobrick+plasmid+ (Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009) eukaryots: CMV-Promoter: konstitutive promoter from cucumber mosaic virus TEV-Protease: Protesase which cuts at the end of specific recognition sites p14*: Protein interacting with SF3b155 (Splicing factor 3b, size: 155kDa) TEV recogn. site: recognition site for TEV-protease eGFP: eukaryotic codon optimized green fluorescent protein SV40PA:polyadenylation signal, needed for translation in eukaryots false false _483_ 0 6378 9 Not in stock false -> "Cut'N'Survive" system false Jara Radeck component2094914 1 BBa_K368010 component2094915 1 BBa_K368011 annotation2094914 1 BBa_K368010 range2094914 1 1 1927 annotation2094915 1 BBa_K368011 range2094915 1 1934 2861 BBa_I714891 1 BBa_I714891 SDY_EGFP 2007-10-19T11:00:00Z 2015-08-31T04:07:48Z combining SD sequence and EGFP gene a stong reporter false false _142_ 0 1911 9 In stock false easy detected reporter true Chunbo Lou annotation1951434 1 SDY range1951434 1 1 16 annotation1951435 1 EGFP range1951435 1 17 736 BBa_I714891_sequence 1 agaggagaaattaagcatggtaagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa BBa_K368010_sequence 1 agaggagaaattaagcatggtaagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagatggcgatgcaagcggccaagagggcgaacattcgacttccacctgaagtaaatcggatattgtatataagaaatttgccatacaaaatcacagctgaagaaatgtatgatatatttgggaaatatggacctattcgtcaaatcagagtggggaacacacctgaaactagaggaacagcttatgtggtctatgaggacatctttgatgccaagaatgcatgtgatcacctatcgggattcaatgtttgtaacagataccttgtggttttgtactataatgccaacagggcatttcagaagatggacacaaagaagaaggaggaacagttgaagcttctcaaggagaaatatggcatcaacacatatcctcccaagatgaaacatcaccatcaccatcaccccatgagcggcctggtgccgcgcggcagcgccatgggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttcgtcgcaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaaggacaccacgactttgcaacaacacctcattgatgggagggacatgatgattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaagctaagagcatgtctagcatggtgtcagacacgagttgcacattcccttcatctgatggtatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaacgctgactcagtattgtgggggggccataaagttttcatggttaaacctgaagaaccttttcagccagttaaggaagcgactcaactcatgaatgaattggtgtactcgcaaggggaaaacttgtacttccaattc BBa_K368019_sequence 1 atggcgatgcaagcggccaagagggcgaacattcgacttccacctgaagtaaatcggatattgtatataagaaatttgccatacaaaatcacagctgaagaaatgtatgatatatttgggaaatatggacctattcgtcaaatcagagtggggaacacacctgaaactagaggaacagcttatgtggtctatgaggacatctttgatgccaagaatgcatgtgatcacctatcgggattcaatgtttgtaacagataccttgtggttttgtactataatgccaacagggcatttcagaagatggacacaaagaagaaggaggaacagttgaagcttctcaaggagaaatatggcatcaacacatatcctcccaagatgaaacatcaccatcaccatcaccccatgagcggcctggtgccgcgcggcagcgccatgggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttcgtcgcaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaaggacaccacgactttgcaacaacacctcattgatgggagggacatgatgattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaagctaagagcatgtctagcatggtgtcagacacgagttgcacattcccttcatctgatggtatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaacgctgactcagtattgtgggggggccataaagttttcatggttaaacctgaagaaccttttcagccagttaaggaagcgactcaactcatgaatgaattggtgtactcgcaaggggaaaacttgtacttccaattc BBa_K368011_sequence 1 atggtaagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagagctagaggatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcctcgag BBa_K368012_sequence 1 agaggagaaattaagcatggtaagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagatggcgatgcaagcggccaagagggcgaacattcgacttccacctgaagtaaatcggatattgtatataagaaatttgccatacaaaatcacagctgaagaaatgtatgatatatttgggaaatatggacctattcgtcaaatcagagtggggaacacacctgaaactagaggaacagcttatgtggtctatgaggacatctttgatgccaagaatgcatgtgatcacctatcgggattcaatgtttgtaacagataccttgtggttttgtactataatgccaacagggcatttcagaagatggacacaaagaagaaggaggaacagttgaagcttctcaaggagaaatatggcatcaacacatatcctcccaagatgaaacatcaccatcaccatcaccccatgagcggcctggtgccgcgcggcagcgccatgggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttcgtcgcaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaaggacaccacgactttgcaacaacacctcattgatgggagggacatgatgattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaagctaagagcatgtctagcatggtgtcagacacgagttgcacattcccttcatctgatggtatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaacgctgactcagtattgtgggggggccataaagttttcatggttaaacctgaagaaccttttcagccagttaaggaagcgactcaactcatgaatgaattggtgtactcgcaaggggaaaacttgtacttccaattctactagatggtaagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagagctagaggatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcctcgag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z