BBa_K371007
type: ComponentDefinition
persistent identity: BBa K371007
display id: BBa_K371007
version: 1
was generated by: iGEM to SBOL conversion
description: pduAB+pduJK+pduN+pduTUV
created: 2010-08-26T11:00:00Z
modified: 2015-05-08T01:12:16Z
mutable provenance: All these genes come from the strain of Citrobacter Freundi(NBRC NO.12681) by PCR
owned by: james
owned by: myers
mutable description: This part consists of four basic part, pduAB, pduJK, pduN and pduTUV. This part encode all proteins used for construct pdu BMC(bacterial microcompartment). All these genes belong to pdu operon, which consist of 21 genes. Some of these genes encode enzymes involved in the utilization of propanediol as carbon resource. Other genes like pduA encode structure proteins, which by unknown method assemble into a large icosahedral protein complex. There are also other similar type of metabolic protein complex, such as carboxysome and ethanolamine utilization complex. They are all belong to BMC(bacterial microcompartment).
discontinued: false
dominant: false
experience: experience/Works
group u list: _498_
m user id: 0
owner id: 3984
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Because the natural sequence of pdu A, pduB and pduJ has a PstI restriction site. Site-mutation method has been applied to remove thess site to meet the RFC10 standard. The sequencing result of some gene shows some silent mutation when compare with the reference sequence we got from NCBI. But these are also few error mutation in pduK and pduTUV. We think the difference between our result and the reference come deprive from the devergence of bacterial strain. We are still do experiment do certify that our system can work well.
star: false
creator: Yang Zhang
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K371001
component: BBa_K371004
component: BBa_K371000
component: BBa_K371005
sequence annotation: BBa_K371001
sequence annotation: BBa_K371000
sequence annotation: BBa_K371004
sequence annotation: BBa_K371005
sequence: https://synbiohub.org/public/igem/BBa_K371007_sequence/1

BBa_K371001
type: Component
persistent identity: component2084042
display id: component2084042
version: 1
top level: BBa_K371007
definition: BBa_K371001
access: public

BBa_K371004
type: Component
persistent identity: component2084056
display id: component2084056
version: 1
top level: BBa_K371007
definition: BBa_K371004
access: public

BBa_K371000
type: Component
persistent identity: component2084051
display id: component2084051
version: 1
top level: BBa_K371007
definition: BBa_K371000
access: public

BBa_K371005
type: Component
persistent identity: component2084069
display id: component2084069
version: 1
top level: BBa_K371007
definition: BBa_K371005
access: public

BBa_K371001
type: SequenceAnnotation
persistent identity: annotation2084042
display id: annotation2084042
version: 1
top level: BBa_K371007
location: https://synbiohub.org/public/igem/BBa_K371007/annotation2084042/range2084042/1
component: BBa_K371001

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type: Range
persistent identity: range2084042
display id: range2084042
version: 1
top level: BBa_K371007
direction: direction/reverse
start: 1
end: 1112
orientation: inline

BBa_K371000
type: SequenceAnnotation
persistent identity: annotation2084051
display id: annotation2084051
version: 1
top level: BBa_K371007
location: https://synbiohub.org/public/igem/BBa_K371007/annotation2084051/range2084051/1
component: BBa_K371000

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type: Range
persistent identity: range2084051
display id: range2084051
version: 1
top level: BBa_K371007
direction: direction/reverse
start: 1121
end: 1903
orientation: inline

BBa_K371004
type: SequenceAnnotation
persistent identity: annotation2084056
display id: annotation2084056
version: 1
top level: BBa_K371007
location: https://synbiohub.org/public/igem/BBa_K371007/annotation2084056/range2084056/1
component: BBa_K371004

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type: Range
persistent identity: range2084056
display id: range2084056
version: 1
top level: BBa_K371007
direction: direction/reverse
start: 1912
end: 2205
orientation: inline

BBa_K371005
type: SequenceAnnotation
persistent identity: annotation2084069
display id: annotation2084069
version: 1
top level: BBa_K371007
location: https://synbiohub.org/public/igem/BBa_K371007/annotation2084069/range2084069/1
component: BBa_K371005

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type: Range
persistent identity: range2084069
display id: range2084069
version: 1
top level: BBa_K371007
direction: direction/reverse
start: 2214
end: 3609
orientation: inline

BBa_K371004
type: ComponentDefinition
persistent identity: BBa K371004
display id: BBa_K371004
version: 1
was generated by: iGEM to SBOL conversion
description: pduN(Propanediol utilization gene N)[RBS+pduN]
created: 2010-08-26T11:00:00Z
modified: 2015-05-08T01:12:15Z
mutable provenance: pduJK come from Citrobacter Freundii genenom(NBRC NO.12681)
owned by: james
owned by: myers
mutable description: Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes, which belongs to a kind of BMC(bacterial microcompartment).Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduN consist of pdu N wich their natural RBS, which got from Citrobacter Freundii by PCR. PduN, together with others struture part of pdu operon, can be used to construct a empty proteinous microcompartment in bacteria.
discontinued: false
dominant: false
experience: experience/Works
group u list: _498_
m user id: 0
owner id: 3984
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: There are some silent mutation in sequencing result of pduN.
star: false
creator: Yang Zhang
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: RBS
sequence annotation: start codon
sequence annotation: pduN conding sequence
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_K371004_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2078724
display id: annotation2078724
version: 1
top level: BBa_K371004
location: https://synbiohub.org/public/igem/BBa_K371004/annotation2078724/range2078724/1
role: SO:0000139
role: feature/rbs

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type: Range
persistent identity: range2078724
display id: range2078724
version: 1
top level: BBa_K371004
direction: direction/forward
start: 4
end: 12
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078723
display id: annotation2078723
version: 1
top level: BBa_K371004
location: https://synbiohub.org/public/igem/BBa_K371004/annotation2078723/range2078723/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range2078723
display id: range2078723
version: 1
top level: BBa_K371004
direction: direction/forward
start: 19
end: 21
orientation: inline

pduN conding sequence
type: SequenceAnnotation
persistent identity: annotation2078725
display id: annotation2078725
version: 1
top level: BBa_K371004
location: https://synbiohub.org/public/igem/BBa_K371004/annotation2078725/range2078725/1
role: SO:0000316
role: feature/cds

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type: Range
persistent identity: range2078725
display id: range2078725
version: 1
top level: BBa_K371004
direction: direction/forward
start: 19
end: 294
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078722
display id: annotation2078722
version: 1
top level: BBa_K371004
location: https://synbiohub.org/public/igem/BBa_K371004/annotation2078722/range2078722/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K371004/annotation2078722/range2078722/1
type: Range
persistent identity: range2078722
display id: range2078722
version: 1
top level: BBa_K371004
direction: direction/forward
start: 292
end: 294
orientation: inline

BBa_K371005
type: ComponentDefinition
persistent identity: BBa K371005
display id: BBa_K371005
version: 1
was generated by: iGEM to SBOL conversion
description: pduTUV(Propanediol utilization gene T+U+V)[RBS+pduT+RBS+pduU+RBS+pduV]
created: 2010-08-26T11:00:00Z
modified: 2015-05-08T01:12:16Z
mutable provenance: pduJK come from Citrobacter Freundii genenom(NBRC NO.12681)
owned by: james
owned by: myers
mutable description: Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes, which belongs to a kind of BMC(bacterial microcompartment).Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduTUV consist of pduT, pduU and pduV wich their corresponding natural RBS, which got from Citrobacter Freundii by PCR. PduTUV, together with other struture parts of pdu operon, can be used to construct a empty proteinous microcompartment in bacteria.
discontinued: false
dominant: false
experience: experience/Works
group u list: _498_
m user id: 0
owner id: 3984
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Sequencing result shows that there are several silent mutation in pduT and pduU. PduV has some error mutation. We are still working on the function assay to certify whether the mutation pduV still function well.
star: false
creator: Yang Zhang
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: RBS
sequence annotation: pduT coding sequence
sequence annotation: start codon
sequence annotation: stop codon
sequence annotation: pduU coding sequence
sequence annotation: pduV coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K371005_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2078729
display id: annotation2078729
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078729/range2078729/1
role: feature/rbs
role: SO:0000139

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type: Range
persistent identity: range2078729
display id: range2078729
version: 1
top level: BBa_K371005
direction: direction/forward
start: 23
end: 28
orientation: inline

pduT coding sequence
type: SequenceAnnotation
persistent identity: annotation2078737
display id: annotation2078737
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078737/range2078737/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K371005/annotation2078737/range2078737/1
type: Range
persistent identity: range2078737
display id: range2078737
version: 1
top level: BBa_K371005
direction: direction/forward
start: 35
end: 589
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078732
display id: annotation2078732
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078732/range2078732/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range2078732
display id: range2078732
version: 1
top level: BBa_K371005
direction: direction/forward
start: 35
end: 47
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2078730
display id: annotation2078730
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078730/range2078730/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K371005/annotation2078730/range2078730/1
type: Range
persistent identity: range2078730
display id: range2078730
version: 1
top level: BBa_K371005
direction: direction/forward
start: 576
end: 582
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078735
display id: annotation2078735
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078735/range2078735/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K371005/annotation2078735/range2078735/1
type: Range
persistent identity: range2078735
display id: range2078735
version: 1
top level: BBa_K371005
direction: direction/forward
start: 587
end: 589
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078733
display id: annotation2078733
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078733/range2078733/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K371005/annotation2078733/range2078733/1
type: Range
persistent identity: range2078733
display id: range2078733
version: 1
top level: BBa_K371005
direction: direction/forward
start: 589
end: 591
orientation: inline

pduU coding sequence
type: SequenceAnnotation
persistent identity: annotation2078727
display id: annotation2078727
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078727/range2078727/1
role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range2078727
display id: range2078727
version: 1
top level: BBa_K371005
direction: direction/forward
start: 589
end: 939
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2078731
display id: annotation2078731
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078731/range2078731/1
role: SO:0000139
role: feature/rbs

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type: Range
persistent identity: range2078731
display id: range2078731
version: 1
top level: BBa_K371005
direction: direction/forward
start: 931
end: 937
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078736
display id: annotation2078736
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078736/range2078736/1
role: SO:0000319
role: feature/stop

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type: Range
persistent identity: range2078736
display id: range2078736
version: 1
top level: BBa_K371005
direction: direction/forward
start: 937
end: 939
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078734
display id: annotation2078734
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078734/range2078734/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range2078734
display id: range2078734
version: 1
top level: BBa_K371005
direction: direction/forward
start: 944
end: 946
orientation: inline

pduV coding sequence
type: SequenceAnnotation
persistent identity: annotation2078728
display id: annotation2078728
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078728/range2078728/1
role: SO:0000316
role: feature/cds

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type: Range
persistent identity: range2078728
display id: range2078728
version: 1
top level: BBa_K371005
direction: direction/forward
start: 944
end: 1396
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078726
display id: annotation2078726
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078726/range2078726/1
role: feature/stop
role: SO:0000319

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type: Range
persistent identity: range2078726
display id: range2078726
version: 1
top level: BBa_K371005
direction: direction/forward
start: 1393
end: 1396
orientation: inline

BBa_K371000
type: ComponentDefinition
persistent identity: BBa K371000
display id: BBa_K371000
version: 1
was generated by: iGEM to SBOL conversion
description: pduJK(Propanediol utilization gene J+K)[RBS+pduJ+RBS+pduK]
created: 2010-08-26T11:00:00Z
modified: 2015-05-08T01:12:15Z
mutable provenance: pduAb come from Citrobacter Freundii genenom(NBRC NO.12681)
owned by: james
owned by: myers
mutable description: Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes. Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduAB consist of pdu A and pdu B wich their natural RBS, which got from Citrobacter Freundii by PCR. PduAB, together with others struture part of pdu operon, can be used to construct a empty proteinous microcompartment in bacteria.
discontinued: false
dominant: false
experience: experience/Works
group u list: _498_
m user id: 0
owner id: 3984
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The sequence results of pduAB, which we got by PCR from Citrobacter Freundii(NBRC NO.12681), are different from the sequence we found on the papar. We suspect that the strain we used is not same with the papar.
star: false
creator: Yang Zhang
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: pduJ RBS
sequence annotation: start codon
sequence annotation: pduJ coding sequence
sequence annotation: stop codon
sequence annotation: pduK RBS
sequence annotation: pduK coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K371000_sequence/1

pduJ RBS
type: SequenceAnnotation
persistent identity: annotation2078670
display id: annotation2078670
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078670/range2078670/1
role: SO:0000139
role: feature/rbs

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type: Range
persistent identity: range2078670
display id: range2078670
version: 1
top level: BBa_K371000
direction: direction/forward
start: 5
end: 12
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078671
display id: annotation2078671
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078671/range2078671/1
role: SO:0000318
role: feature/start

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type: Range
persistent identity: range2078671
display id: range2078671
version: 1
top level: BBa_K371000
direction: direction/forward
start: 19
end: 21
orientation: inline

pduJ coding sequence
type: SequenceAnnotation
persistent identity: annotation2078672
display id: annotation2078672
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078672/range2078672/1
role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range2078672
display id: range2078672
version: 1
top level: BBa_K371000
direction: direction/forward
start: 19
end: 294
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078674
display id: annotation2078674
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078674/range2078674/1
role: feature/stop
role: SO:0000319

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type: Range
persistent identity: range2078674
display id: range2078674
version: 1
top level: BBa_K371000
direction: direction/forward
start: 292
end: 294
orientation: inline

pduK RBS
type: SequenceAnnotation
persistent identity: annotation2078675
display id: annotation2078675
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078675/range2078675/1
role: feature/rbs
role: SO:0000139

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type: Range
persistent identity: range2078675
display id: range2078675
version: 1
top level: BBa_K371000
direction: direction/forward
start: 307
end: 312
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078676
display id: annotation2078676
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078676/range2078676/1
role: SO:0000318
role: feature/start

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type: Range
persistent identity: range2078676
display id: range2078676
version: 1
top level: BBa_K371000
direction: direction/forward
start: 319
end: 321
orientation: inline

pduK coding sequence
type: SequenceAnnotation
persistent identity: annotation2078673
display id: annotation2078673
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078673/range2078673/1
role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range2078673
display id: range2078673
version: 1
top level: BBa_K371000
direction: direction/forward
start: 319
end: 783
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078677
display id: annotation2078677
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078677/range2078677/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K371000/annotation2078677/range2078677/1
type: Range
persistent identity: range2078677
display id: range2078677
version: 1
top level: BBa_K371000
direction: direction/forward
start: 781
end: 783
orientation: inline

BBa_K371001
type: ComponentDefinition
persistent identity: BBa K371001
display id: BBa_K371001
version: 1
was generated by: iGEM to SBOL conversion
description: pduAB(Propanediol utilization gene A+B)[RBS+pduA+RBS+pduB]
created: 2010-08-26T11:00:00Z
modified: 2015-05-08T01:12:15Z
mutable provenance: pduJK come from Citrobacter Freundii genenom(NBRC NO.12681)
owned by: james
owned by: myers
mutable description: Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes. Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduAB consist of pdu A and pdu B wich their corresponding natural RBS, which got from Citrobacter Freundii by PCR. PduAB, together with others struture part of pdu operon such as pdu JK, can be used to construct a empty proteinous microcompartment in bacteria.
discontinued: false
dominant: false
experience: experience/Works
group u list: _498_
m user id: 0
owner id: 3984
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The sequencing result show that the gene of pduAB has some silent mutations, which have no effect on the function of protein.
star: false
creator: Yang Zhang
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: start codon
sequence annotation: pduA coding sequence
sequence annotation: stop codon
sequence annotation: pduB coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K371001_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2078678
display id: annotation2078678
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078678/range2078678/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K371001/annotation2078678/range2078678/1
type: Range
persistent identity: range2078678
display id: range2078678
version: 1
top level: BBa_K371001
direction: direction/forward
start: 6
end: 11
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078685
display id: annotation2078685
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078685/range2078685/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K371001/annotation2078685/range2078685/1
type: Range
persistent identity: range2078685
display id: range2078685
version: 1
top level: BBa_K371001
direction: direction/forward
start: 19
end: 21
orientation: inline

pduA coding sequence
type: SequenceAnnotation
persistent identity: annotation2078679
display id: annotation2078679
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078679/range2078679/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K371001/annotation2078679/range2078679/1
type: Range
persistent identity: range2078679
display id: range2078679
version: 1
top level: BBa_K371001
direction: direction/forward
start: 19
end: 300
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2078683
display id: annotation2078683
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078683/range2078683/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K371001/annotation2078683/range2078683/1
type: Range
persistent identity: range2078683
display id: range2078683
version: 1
top level: BBa_K371001
direction: direction/forward
start: 285
end: 292
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078681
display id: annotation2078681
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078681/range2078681/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K371001/annotation2078681/range2078681/1
type: Range
persistent identity: range2078681
display id: range2078681
version: 1
top level: BBa_K371001
direction: direction/forward
start: 298
end: 300
orientation: inline

pduB coding sequence
type: SequenceAnnotation
persistent identity: annotation2078682
display id: annotation2078682
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078682/range2078682/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K371001/annotation2078682/range2078682/1
type: Range
persistent identity: range2078682
display id: range2078682
version: 1
top level: BBa_K371001
direction: direction/forward
start: 300
end: 1112
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078680
display id: annotation2078680
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078680/range2078680/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K371001/annotation2078680/range2078680/1
type: Range
persistent identity: range2078680
display id: range2078680
version: 1
top level: BBa_K371001
direction: direction/forward
start: 300
end: 302
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078684
display id: annotation2078684
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078684/range2078684/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K371001/annotation2078684/range2078684/1
type: Range
persistent identity: range2078684
display id: range2078684
version: 1
top level: BBa_K371001
direction: direction/forward
start: 1110
end: 1112
orientation: inline

https://synbiohub.org/public/igem/BBa_K371007_sequence/1
type: Sequence
persistent identity: BBa K371007 sequence
display id: BBa_K371007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacccaggcgaggtctttatgcaacaagaagcgttaggaatggtagaaaccaaaggcttgacagcagccatagaggccgcagatgcaatggtgaagtcagccaatgtaatgctggtcggctacgaaaaaattggttcggggctggtaacagttattgtccgcggcgatgtcggcgcagtcaaagcagcaacagatgccggtgccgccgcagcccgtaatgtgggagaagtgaaagccgtacacgttatcccacgcccgcataccgatgtagaaaaaatcttaccgaagggaattagctaatgagcagcaatgaactggttgaacagatcatggcgcaggtgattgctcgcgtggcaacgccggaacagcaggctatcccagaaaataatcacccaacacgagaaacggctatggcagagaaaagctgtagtttaacggagtttgtcggtaccgcgattggcgacaccgtcggtctggtaatcgccaacgtagacagtgccctactggacgcaatgaaacttgaaaaacgctatcgctccattggcatccttggcgcccgtactggtgcgggtccacacatcatggccgctgatgaagcggtgaaagccaccaatactgaagtcgtcagtattgagttaccacgtgataccaaaggcggcgcaggtcatggctcgctgattattcttggcggcaacgatgtttccgacgtgaaacgcggcattgaagtcgcactgaaagagctggatcgcacctttggcgacgtttatgccaacgaagccggtcacatcgaaatgcaatacaccgcacgcgccagctacgcacttgaaaaagcctttggcgcaccgattggtcgtgcctgtggcgtcatcgtcggcgcaccagcatccgttggtgtcctgatggctgatactgccctgaaatccgccaacgtggaagttgtggcttacagctcccctgctcatggcaccagcttcagtaacgaagccattctggttatttcgggtgactctggcgctgttcgtcaggctgttatctctgcccgtgaaatcggtaaaaccgtactcgggaccctcggctcagagccgaaaaacgatcgtccgtcctacatctgatactagagccacaggagaaaagcagtatgaataacgcactgggactggttgaaacaaaagggcttgtcggcgctattgaagccgctgatgccatggtgaaatccgcaaacgtgcagttggttggttacgaaaaaatcggctctggcctgatcaacgttatggttcgcggtgatgtcggcgcggtgaaagcagccgtagatgctggaagcgcagcagcaagcgccgttggtgaggtgaaatcctgccacgttatcccgcgtccgcacagcgacgttgaagccattttacctaaatccgcataagtcgttgcaaccaaggagcacagcgtgaagcaatcactgggattacttgaagttagtggtctggcattagccatcagttgcgcggacgtcatggcgaaagccgcctccatcacgctggtgggcctcgaaaaaaccaacggctcaggctggacggtgatcaagataatcggggatgtggcctccgtccaggcggccatttccactggtgtcagtttcgctgaccagcgagatggactggtggctcacaaagtcatttccagacccggggacgggatcctgtcgcatagcgttgtcctggagcctgaaccaacgcccgaccccataccagccataccacatgaagaaatccctgaggaccatgcggcacccgaagcaccgcaagatgcagaattgattagctgcaatttgtgtcttgatcctgcctgccctcgccaaaaaggtgagccgcgcacactttgtcttcactccggcaaacgaggtgaagcgtgatactagagattaagcaggagtgagctatgcatctggcacgggttacaggcgttgtggtttccacgcaaaaatctccatcactggtgggaaaaaaact…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371004_sequence/1
type: Sequence
persistent identity: BBa K371004 sequence
display id: BBa_K371004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaagcaggagtgagctatgcatctggcacgggttacaggcgttgtggtttccacgcaaaaatctccatcactggtgggaaaaaaactgttgctggtacgtcgggtaagtgctgacggagagcttcctgcgtctccagtgagtggagatgaagtcgctgttgattctgtcggcgcggggaccggcgaactggtattactcagcagcgggtccagcgccagacacgttttttccggcccgaatgaggccattgatctggctatcgtcggcattgtcgacacgctttctcgttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371005_sequence/1
type: Sequence
persistent identity: BBa K371005 sequence
display id: BBa_K371005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgagcaatccatcacggtaataagaggttaatcatgtctcaggctatagggattttagaactcaccagcattgccaaaggaatggaagctggcgatgccatgttaaaaagcgcgaatgtgaatttactggtcagcaagaccatctgcccgggaaaatttctactcatgctcggtggtgacgtaggtgccgtacaacaggcgattgccactggaacatctcttgctggcgatatgctcgttgatagtctggtactccccaacattcatgccagcgtactacccgcaatcagcggactaaacagcgtagataagcgtcaggcagttggtattgtcgaaacatggagcgttgctgcctgcatctgcgccgccgaccgggcggttaaagcctccaacgtcacgctggtccgcgtccgtatggcatttggtattggcggcaagtgttacatggtcgtagctggcgatgtatccgatgtgaataacgcggtgacggttgccagtgaaagtgcgggtgaaaaaggcctgctggtttaccgctcggtcatcccccgtccacacgaaagcatgtggcgacaaatggtggaaggataatggaaagacaacccaccacggatcgtatgattcaggaatatgttcctggcaaacaggtcacgttggcgcaccttatcgccaatcccggtaaggatttgttcaagaaactgggattacctgattcggtttctgcgatcggtattctgaccattacccctagcgaagcctcaatcatcgcctgtgatatcgccacgaaatcaggggcggtagagattggttttctcgaccggtttaccggcgcggtggtactgacgggcgatgtttcagccgttgagtacgcgctgaaacaggtaacccgaacgctgggcgaaatgatgcgttttaccgcgtgtcccatcacccggacgtaatctcgtgaaacgtatcatgctaattggccccagccagtgcggtaaaacgtcgctcacacagtgcatgcgcggagaggtgctccactatcagaagacccaggccatagtctggtcacctacgacaatagacacaccgggtgaatatcttgagaaccgctgcctgtacagtgcgctgctggccagcgcctgtgaagcggacattattgcgctggtactcaatgctgacgcgccgtggtcaccattttctcccggttttaccggaccaatgaaccgacccgttattggcctcgttaccaaagccgatttagccaacccgcagcgtatttctcttgtggagagttggttagctcaggctggtgcgcaaaaagtatttgtcaccagcgcgctggagaataccggggtcgatgagatgttcatttttctgaatgcaaaggaatcttcctgtctcacaaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371000_sequence/1
type: Sequence
persistent identity: BBa K371000 sequence
display id: BBa_K371000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaggagaaaagcagtatgaataacgcactgggactggttgaaacaaaagggcttgtcggcgctattgaagccgctgatgccatggtgaaatccgcaaacgtgcagttggttggttacgaaaaaatcggctctggcctgatcaacgttatggttcgcggtgatgtcggcgcggtgaaagcagccgtagatgctggaagcgcagcagcaagcgccgttggtgaggtgaaatcctgccacgttatcccgcgtccgcacagcgacgttgaagccattttacctaaatccgcataagtcgttgcaaccaaggagcacagcgtgaagcaatcactgggattacttgaagttagtggtctggcattagccatcagttgcgcggacgtcatggcgaaagccgcctccatcacgctggtgggcctcgaaaaaaccaacggctcaggctggacggtgatcaagataatcggggatgtggcctccgtccaggcggccatttccactggtgtcagtttcgctgaccagcgagatggactggtggctcacaaagtcatttccagacccggggacgggatcctgtcgcatagcgttgtcctggagcctgaaccaacgcccgaccccataccagccataccacatgaagaaatccctgaggaccatgcggcacccgaagcaccgcaagatgcagaattgattagctgcaatttgtgtcttgatcctgcctgccctcgccaaaaaggtgagccgcgcacactttgtcttcactccggcaaacgaggtgaagcgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371001_sequence/1
type: Sequence
persistent identity: BBa K371001 sequence
display id: BBa_K371001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacccaggcgaggtctttatgcaacaagaagcgttaggaatggtagaaaccaaaggcttgacagcagccatagaggccgcagatgcaatggtgaagtcagccaatgtaatgctggtcggctacgaaaaaattggttcggggctggtaacagttattgtccgcggcgatgtcggcgcagtcaaagcagcaacagatgccggtgccgccgcagcccgtaatgtgggagaagtgaaagccgtacacgttatcccacgcccgcataccgatgtagaaaaaatcttaccgaagggaattagctaatgagcagcaatgaactggttgaacagatcatggcgcaggtgattgctcgcgtggcaacgccggaacagcaggctatcccagaaaataatcacccaacacgagaaacggctatggcagagaaaagctgtagtttaacggagtttgtcggtaccgcgattggcgacaccgtcggtctggtaatcgccaacgtagacagtgccctactggacgcaatgaaacttgaaaaacgctatcgctccattggcatccttggcgcccgtactggtgcgggtccacacatcatggccgctgatgaagcggtgaaagccaccaatactgaagtcgtcagtattgagttaccacgtgataccaaaggcggcgcaggtcatggctcgctgattattcttggcggcaacgatgtttccgacgtgaaacgcggcattgaagtcgcactgaaagagctggatcgcacctttggcgacgtttatgccaacgaagccggtcacatcgaaatgcaatacaccgcacgcgccagctacgcacttgaaaaagcctttggcgcaccgattggtcgtgcctgtggcgtcatcgtcggcgcaccagcatccgttggtgtcctgatggctgatactgccctgaaatccgccaacgtggaagttgtggcttacagctcccctgctcatggcaccagcttcagtaacgaagccattctggttatttcgggtgactctggcgctgttcgtcaggctgttatctctgcccgtgaaatcggtaaaaccgtactcgggaccctcggctcagagccgaaaaacgatcgtccgtcctacatctga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z