All these genes come from the strain of Citrobacter Freundi(NBRC NO.12681) by PCR
This part consists of four basic part, pduAB, pduJK, pduN and pduTUV. This part encode all proteins used for construct pdu BMC(bacterial microcompartment). All these genes belong to pdu operon, which consist of 21 genes. Some of these genes encode enzymes involved in the utilization of propanediol as carbon resource. Other genes like pduA encode structure proteins, which by unknown method assemble into a large icosahedral protein complex. There are also other similar type of metabolic protein complex, such as carboxysome and ethanolamine utilization complex. They are all belong to BMC(bacterial microcompartment).
Because the natural sequence of pdu A, pduB and pduJ has a PstI restriction site. Site-mutation method has been applied to remove thess site to meet the RFC10 standard. The sequencing result of some gene shows some silent mutation when compare with the reference sequence we got from NCBI. But these are also few error mutation in pduK and pduTUV. We think the difference between our result and the reference come deprive from the devergence of bacterial strain. We are still do experiment do certify that our system can work well.
pduN(Propanediol utilization gene N)[RBS+pduN]
pduJK come from Citrobacter Freundii genenom(NBRC NO.12681)
Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes, which belongs to a kind of BMC(bacterial microcompartment).Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduN consist of pdu N wich their natural RBS, which got from Citrobacter Freundii by PCR. PduN, together with others struture part of pdu operon, can be used to construct a empty proteinous microcompartment in bacteria.
There are some silent mutation in sequencing result of pduN.
pduTUV(Propanediol utilization gene T+U+V)[RBS+pduT+RBS+pduU+RBS+pduV]
Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes, which belongs to a kind of BMC(bacterial microcompartment).Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduTUV consist of pduT, pduU and pduV wich their corresponding natural RBS, which got from Citrobacter Freundii by PCR. PduTUV, together with other struture parts of pdu operon, can be used to construct a empty proteinous microcompartment in bacteria.
Sequencing result shows that there are several silent mutation in pduT and pduU. PduV has some error mutation. We are still working on the function assay to certify whether the mutation pduV still function well.
pduJK(Propanediol utilization gene J+K)[RBS+pduJ+RBS+pduK]
pduAb come from Citrobacter Freundii genenom(NBRC NO.12681)
Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes. Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduAB consist of pdu A and pdu B wich their natural RBS, which got from Citrobacter Freundii by PCR. PduAB, together with others struture part of pdu operon, can be used to construct a empty proteinous microcompartment in bacteria.
The sequence results of pduAB, which we got by PCR from Citrobacter Freundii(NBRC NO.12681), are different from the sequence we found on the papar. We suspect that the strain we used is not same with the papar.
pduAB(Propanediol utilization gene A+B)[RBS+pduA+RBS+pduB]
Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes. Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduAB consist of pdu A and pdu B wich their corresponding natural RBS, which got from Citrobacter Freundii by PCR. PduAB, together with others struture part of pdu operon such as pdu JK, can be used to construct a empty proteinous microcompartment in bacteria.
The sequencing result show that the gene of pduAB has some silent mutations, which have no effect on the function of protein.
Conversion of the iGEM parts registry to SBOL2.1