LOCUS BBa_K371019 3817 bp DNA linear 01-Jul-2024 DEFINITION pdu(Propanediol utilization)BMC shell gene under Plac control ACCESSION BBa_K371019 VERSION BBa_K371019.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 214..219 /label=RBS CDS 227..508 /label=pduA coding sequence misc_feature 508..510 /label=start codon misc_feature 506..508 /label=stop codon CDS 508..1320 /label=pduB coding sequence RBS 493..500 /label=RBS misc_feature 1318..1320 /label=stop codon misc_feature 227..229 /label=start codon misc_feature 209..1320 /label=BBa_K371001 RBS 1333..1340 /label=pduJ RBS misc_feature 1347..1349 /label=start codon CDS 1347..1622 /label=pduJ coding sequence CDS 1647..2111 /label=pduK coding sequence misc_feature 1620..1622 /label=stop codon RBS 1635..1640 /label=pduK RBS misc_feature 1647..1649 /label=start codon misc_feature 2109..2111 /label=stop codon misc_feature 1329..2111 /label=BBa_K371000 misc_feature 2411..2413 /label=stop codon misc_feature 2138..2140 /label=start codon RBS 2123..2131 /label=RBS CDS 2138..2413 /label=pduN conding sequence misc_feature 2120..2413 /label=BBa_K371004 misc_feature 3814..3817 /label=stop codon CDS 3010..3360 /label=pduU coding sequence CDS 3365..3817 /label=pduV coding sequence RBS 2444..2449 /label=RBS RBS 2997..3003 /label=RBS RBS 3352..3358 /label=RBS misc_feature 2456..2468 /label=start codon misc_feature 3010..3012 /label=start codon misc_feature 3365..3367 /label=start codon misc_feature 3008..3010 /label=stop codon misc_feature 3358..3360 /label=stop codon CDS 2456..3010 /label=pduT coding sequence misc_feature 2422..3817 /label=BBa_K371005 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa cccaggcgag gtctttatgc aacaagaagc 241 gttaggaatg gtagaaacca aaggcttgac agcagccata gaggccgcag atgcaatggt 301 gaagtcagcc aatgtaatgc tggtcggcta cgaaaaaatt ggttcggggc tggtaacagt 361 tattgtccgc ggcgatgtcg gcgcagtcaa agcagcaaca gatgccggtg ccgccgcagc 421 ccgtaatgtg ggagaagtga aagccgtaca cgttatccca cgcccgcata ccgatgtaga 481 aaaaatctta ccgaagggaa ttagctaatg agcagcaatg aactggttga acagatcatg 541 gcgcaggtga ttgctcgcgt ggcaacgccg gaacagcagg ctatcccaga aaataatcac 601 ccaacacgag aaacggctat ggcagagaaa agctgtagtt taacggagtt tgtcggtacc 661 gcgattggcg acaccgtcgg tctggtaatc gccaacgtag acagtgccct actggacgca 721 atgaaacttg aaaaacgcta tcgctccatt ggcatccttg gcgcccgtac tggtgcgggt 781 ccacacatca tggccgctga tgaagcggtg aaagccacca atactgaagt cgtcagtatt 841 gagttaccac gtgataccaa aggcggcgca ggtcatggct cgctgattat tcttggcggc 901 aacgatgttt ccgacgtgaa acgcggcatt gaagtcgcac tgaaagagct ggatcgcacc 961 tttggcgacg tttatgccaa cgaagccggt cacatcgaaa tgcaatacac cgcacgcgcc 1021 agctacgcac ttgaaaaagc ctttggcgca ccgattggtc gtgcctgtgg cgtcatcgtc 1081 ggcgcaccag catccgttgg tgtcctgatg gctgatactg ccctgaaatc cgccaacgtg 1141 gaagttgtgg cttacagctc ccctgctcat ggcaccagct tcagtaacga agccattctg 1201 gttatttcgg gtgactctgg cgctgttcgt caggctgtta tctctgcccg tgaaatcggt 1261 aaaaccgtac tcgggaccct cggctcagag ccgaaaaacg atcgtccgtc ctacatctga 1321 tactagagcc acaggagaaa agcagtatga ataacgcact gggactggtt gaaacaaaag 1381 ggcttgtcgg cgctattgaa gccgctgatg ccatggtgaa atccgcaaac gtgcagttgg 1441 ttggttacga aaaaatcggc tctggcctga tcaacgttat ggttcgcggt gatgtcggcg 1501 cggtgaaagc agccgtagat gctggaagcg cagcagcaag cgccgttggt gaggtgaaat 1561 cctgccacgt tatcccgcgt ccgcacagcg acgttgaagc cattttacct aaatccgcat 1621 aagtcgttgc aaccaaggag cacagcgtga agcaatcact gggattactt gaagttagtg 1681 gtctggcatt agccatcagt tgcgcggacg tcatggcgaa agccgcctcc atcacgctgg 1741 tgggcctcga aaaaaccaac ggctcaggct ggacggtgat caagataatc ggggatgtgg 1801 cctccgtcca ggcggccatt tccactggtg tcagtttcgc tgaccagcga gatggactgg 1861 tggctcacaa agtcatttcc agacccgggg acgggatcct gtcgcatagc gttgtcctgg 1921 agcctgaacc aacgcccgac cccataccag ccataccaca tgaagaaatc cctgaggacc 1981 atgcggcacc cgaagcaccg caagatgcag aattgattag ctgcaatttg tgtcttgatc 2041 ctgcctgccc tcgccaaaaa ggtgagccgc gcacactttg tcttcactcc ggcaaacgag 2101 gtgaagcgtg atactagaga ttaagcagga gtgagctatg catctggcac gggttacagg 2161 cgttgtggtt tccacgcaaa aatctccatc actggtggga aaaaaactgt tgctggtacg 2221 tcgggtaagt gctgacggag agcttcctgc gtctccagtg agtggagatg aagtcgctgt 2281 tgattctgtc ggcgcgggga ccggcgaact ggtattactc agcagcgggt ccagcgccag 2341 acacgttttt tccggcccga atgaggccat tgatctggct atcgtcggca ttgtcgacac 2401 gctttctcgt tagtactaga gctgagcaat ccatcacggt aataagaggt taatcatgtc 2461 tcaggctata gggattttag aactcaccag cattgccaaa ggaatggaag ctggcgatgc 2521 catgttaaaa agcgcgaatg tgaatttact ggtcagcaag accatctgcc cgggaaaatt 2581 tctactcatg ctcggtggtg acgtaggtgc cgtacaacag gcgattgcca ctggaacatc 2641 tcttgctggc gatatgctcg ttgatagtct ggtactcccc aacattcatg ccagcgtact 2701 acccgcaatc agcggactaa acagcgtaga taagcgtcag gcagttggta ttgtcgaaac 2761 atggagcgtt gctgcctgca tctgcgccgc cgaccgggcg gttaaagcct ccaacgtcac 2821 gctggtccgc gtccgtatgg catttggtat tggcggcaag tgttacatgg tcgtagctgg 2881 cgatgtatcc gatgtgaata acgcggtgac ggttgccagt gaaagtgcgg gtgaaaaagg 2941 cctgctggtt taccgctcgg tcatcccccg tccacacgaa agcatgtggc gacaaatggt 3001 ggaaggataa tggaaagaca acccaccacg gatcgtatga ttcaggaata tgttcctggc 3061 aaacaggtca cgttggcgca ccttatcgcc aatcccggta aggatttgtt caagaaactg 3121 ggattacctg attcggtttc tgcgatcggt attctgacca ttacccctag cgaagcctca 3181 atcatcgcct gtgatatcgc cacgaaatca ggggcggtag agattggttt tctcgaccgg 3241 tttaccggcg cggtggtact gacgggcgat gtttcagccg ttgagtacgc gctgaaacag 3301 gtaacccgaa cgctgggcga aatgatgcgt tttaccgcgt gtcccatcac ccggacgtaa 3361 tctcgtgaaa cgtatcatgc taattggccc cagccagtgc ggtaaaacgt cgctcacaca 3421 gtgcatgcgc ggagaggtgc tccactatca gaagacccag gccatagtct ggtcacctac 3481 gacaatagac acaccgggtg aatatcttga gaaccgctgc ctgtacagtg cgctgctggc 3541 cagcgcctgt gaagcggaca ttattgcgct ggtactcaat gctgacgcgc cgtggtcacc 3601 attttctccc ggttttaccg gaccaatgaa ccgacccgtt attggcctcg ttaccaaagc 3661 cgatttagcc aacccgcagc gtatttctct tgtggagagt tggttagctc aggctggtgc 3721 gcaaaaagta tttgtcacca gcgcgctgga gaataccggg gtcgatgaga tgttcatttt 3781 tctgaatgca aaggaatctt cctgtctcac aaaataa //