##gff-version 3
##sequence-region BBa_K371019 1 3817
BBa_K371019	.	engineered_region	2422	3817	.	+	0	ID=BBa_K371005;Name=BBa_K371005
BBa_K371019	.	CDS	3010	3360	.	+	0	ID=annotation2078727;Name=pduU coding sequence;Parent=BBa_K371005
BBa_K371019	.	ribosome_entry_site	3352	3358	.	+	0	ID=annotation2078731;Name=RBS;Parent=BBa_K371005
BBa_K371019	.	stop_codon	3814	3817	.	+	0	ID=annotation2078726;Name=stop codon;Parent=BBa_K371005
BBa_K371019	.	CDS	2456	3010	.	+	0	ID=annotation2078737;Name=pduT coding sequence;Parent=BBa_K371005
BBa_K371019	.	ribosome_entry_site	2444	2449	.	+	0	ID=annotation2078729;Name=RBS;Parent=BBa_K371005
BBa_K371019	.	stop_codon	3008	3010	.	+	0	ID=annotation2078735;Name=stop codon;Parent=BBa_K371005
BBa_K371019	.	start_codon	3365	3367	.	+	0	ID=annotation2078734;Name=start codon;Parent=BBa_K371005
BBa_K371019	.	ribosome_entry_site	2997	3003	.	+	0	ID=annotation2078730;Name=RBS;Parent=BBa_K371005
BBa_K371019	.	CDS	3365	3817	.	+	0	ID=annotation2078728;Name=pduV coding sequence;Parent=BBa_K371005
BBa_K371019	.	start_codon	3010	3012	.	+	0	ID=annotation2078733;Name=start codon;Parent=BBa_K371005
BBa_K371019	.	start_codon	2456	2468	.	+	0	ID=annotation2078732;Name=start codon;Parent=BBa_K371005
BBa_K371019	.	stop_codon	3358	3360	.	+	0	ID=annotation2078736;Name=stop codon;Parent=BBa_K371005
BBa_K371019	.	engineered_region	209	1320	.	+	0	ID=BBa_K371001;Name=BBa_K371001
BBa_K371019	.	stop_codon	1318	1320	.	+	0	ID=annotation2078684;Name=stop codon;Parent=BBa_K371001
BBa_K371019	.	stop_codon	506	508	.	+	0	ID=annotation2078681;Name=stop codon;Parent=BBa_K371001
BBa_K371019	.	CDS	508	1320	.	+	0	ID=annotation2078682;Name=pduB coding sequence;Parent=BBa_K371001
BBa_K371019	.	start_codon	508	510	.	+	0	ID=annotation2078680;Name=start codon;Parent=BBa_K371001
BBa_K371019	.	ribosome_entry_site	493	500	.	+	0	ID=annotation2078683;Name=RBS;Parent=BBa_K371001
BBa_K371019	.	start_codon	227	229	.	+	0	ID=annotation2078685;Name=start codon;Parent=BBa_K371001
BBa_K371019	.	ribosome_entry_site	214	219	.	+	0	ID=annotation2078678;Name=RBS;Parent=BBa_K371001
BBa_K371019	.	CDS	227	508	.	+	0	ID=annotation2078679;Name=pduA coding sequence;Parent=BBa_K371001
BBa_K371019	.	engineered_region	2120	2413	.	+	0	ID=BBa_K371004;Name=BBa_K371004
BBa_K371019	.	stop_codon	2411	2413	.	+	0	ID=annotation2078722;Name=stop codon;Parent=BBa_K371004
BBa_K371019	.	CDS	2138	2413	.	+	0	ID=annotation2078725;Name=pduN conding sequence;Parent=BBa_K371004
BBa_K371019	.	ribosome_entry_site	2123	2131	.	+	0	ID=annotation2078724;Name=RBS;Parent=BBa_K371004
BBa_K371019	.	start_codon	2138	2140	.	+	0	ID=annotation2078723;Name=start codon;Parent=BBa_K371004
BBa_K371019	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K371019	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K371019	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K371019	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K371019	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K371019	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K371019	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K371019	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K371019	.	engineered_region	1329	2111	.	+	0	ID=BBa_K371000;Name=BBa_K371000
BBa_K371019	.	start_codon	1647	1649	.	+	0	ID=annotation2078676;Name=start codon;Parent=BBa_K371000
BBa_K371019	.	ribosome_entry_site	1333	1340	.	+	0	ID=annotation2078670;Name=pduJ RBS;Parent=BBa_K371000
BBa_K371019	.	ribosome_entry_site	1635	1640	.	+	0	ID=annotation2078675;Name=pduK RBS;Parent=BBa_K371000
BBa_K371019	.	CDS	1347	1622	.	+	0	ID=annotation2078672;Name=pduJ coding sequence;Parent=BBa_K371000
BBa_K371019	.	stop_codon	2109	2111	.	+	0	ID=annotation2078677;Name=stop codon;Parent=BBa_K371000
BBa_K371019	.	stop_codon	1620	1622	.	+	0	ID=annotation2078674;Name=stop codon;Parent=BBa_K371000
BBa_K371019	.	start_codon	1347	1349	.	+	0	ID=annotation2078671;Name=start codon;Parent=BBa_K371000
BBa_K371019	.	CDS	1647	2111	.	+	0	ID=annotation2078673;Name=pduK coding sequence;Parent=BBa_K371000
>BBa_K371019
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaacccaggcgaggtctttatgcaacaaga
agcgttaggaatggtagaaaccaaaggcttgacagcagccatagaggccgcagatgcaatggtgaagtcagccaatgta
atgctggtcggctacgaaaaaattggttcggggctggtaacagttattgtccgcggcgatgtcggcgcagtcaaagcag
caacagatgccggtgccgccgcagcccgtaatgtgggagaagtgaaagccgtacacgttatcccacgcccgcataccga
tgtagaaaaaatcttaccgaagggaattagctaatgagcagcaatgaactggttgaacagatcatggcgcaggtgattg
ctcgcgtggcaacgccggaacagcaggctatcccagaaaataatcacccaacacgagaaacggctatggcagagaaaag
ctgtagtttaacggagtttgtcggtaccgcgattggcgacaccgtcggtctggtaatcgccaacgtagacagtgcccta
ctggacgcaatgaaacttgaaaaacgctatcgctccattggcatccttggcgcccgtactggtgcgggtccacacatca
tggccgctgatgaagcggtgaaagccaccaatactgaagtcgtcagtattgagttaccacgtgataccaaaggcggcgc
aggtcatggctcgctgattattcttggcggcaacgatgtttccgacgtgaaacgcggcattgaagtcgcactgaaagag
ctggatcgcacctttggcgacgtttatgccaacgaagccggtcacatcgaaatgcaatacaccgcacgcgccagctacg
cacttgaaaaagcctttggcgcaccgattggtcgtgcctgtggcgtcatcgtcggcgcaccagcatccgttggtgtcct
gatggctgatactgccctgaaatccgccaacgtggaagttgtggcttacagctcccctgctcatggcaccagcttcagt
aacgaagccattctggttatttcgggtgactctggcgctgttcgtcaggctgttatctctgcccgtgaaatcggtaaaa
ccgtactcgggaccctcggctcagagccgaaaaacgatcgtccgtcctacatctgatactagagccacaggagaaaagc
agtatgaataacgcactgggactggttgaaacaaaagggcttgtcggcgctattgaagccgctgatgccatggtgaaat
ccgcaaacgtgcagttggttggttacgaaaaaatcggctctggcctgatcaacgttatggttcgcggtgatgtcggcgc
ggtgaaagcagccgtagatgctggaagcgcagcagcaagcgccgttggtgaggtgaaatcctgccacgttatcccgcgt
ccgcacagcgacgttgaagccattttacctaaatccgcataagtcgttgcaaccaaggagcacagcgtgaagcaatcac
tgggattacttgaagttagtggtctggcattagccatcagttgcgcggacgtcatggcgaaagccgcctccatcacgct
ggtgggcctcgaaaaaaccaacggctcaggctggacggtgatcaagataatcggggatgtggcctccgtccaggcggcc
atttccactggtgtcagtttcgctgaccagcgagatggactggtggctcacaaagtcatttccagacccggggacggga
tcctgtcgcatagcgttgtcctggagcctgaaccaacgcccgaccccataccagccataccacatgaagaaatccctga
ggaccatgcggcacccgaagcaccgcaagatgcagaattgattagctgcaatttgtgtcttgatcctgcctgccctcgc
caaaaaggtgagccgcgcacactttgtcttcactccggcaaacgaggtgaagcgtgatactagagattaagcaggagtg
agctatgcatctggcacgggttacaggcgttgtggtttccacgcaaaaatctccatcactggtgggaaaaaaactgttg
ctggtacgtcgggtaagtgctgacggagagcttcctgcgtctccagtgagtggagatgaagtcgctgttgattctgtcg
gcgcggggaccggcgaactggtattactcagcagcgggtccagcgccagacacgttttttccggcccgaatgaggccat
tgatctggctatcgtcggcattgtcgacacgctttctcgttagtactagagctgagcaatccatcacggtaataagagg
ttaatcatgtctcaggctatagggattttagaactcaccagcattgccaaaggaatggaagctggcgatgccatgttaa
aaagcgcgaatgtgaatttactggtcagcaagaccatctgcccgggaaaatttctactcatgctcggtggtgacgtagg
tgccgtacaacaggcgattgccactggaacatctcttgctggcgatatgctcgttgatagtctggtactccccaacatt
catgccagcgtactacccgcaatcagcggactaaacagcgtagataagcgtcaggcagttggtattgtcgaaacatgga
gcgttgctgcctgcatctgcgccgccgaccgggcggttaaagcctccaacgtcacgctggtccgcgtccgtatggcatt
tggtattggcggcaagtgttacatggtcgtagctggcgatgtatccgatgtgaataacgcggtgacggttgccagtgaa
agtgcgggtgaaaaaggcctgctggtttaccgctcggtcatcccccgtccacacgaaagcatgtggcgacaaatggtgg
aaggataatggaaagacaacccaccacggatcgtatgattcaggaatatgttcctggcaaacaggtcacgttggcgcac
cttatcgccaatcccggtaaggatttgttcaagaaactgggattacctgattcggtttctgcgatcggtattctgacca
ttacccctagcgaagcctcaatcatcgcctgtgatatcgccacgaaatcaggggcggtagagattggttttctcgaccg
gtttaccggcgcggtggtactgacgggcgatgtttcagccgttgagtacgcgctgaaacaggtaacccgaacgctgggc
gaaatgatgcgttttaccgcgtgtcccatcacccggacgtaatctcgtgaaacgtatcatgctaattggccccagccag
tgcggtaaaacgtcgctcacacagtgcatgcgcggagaggtgctccactatcagaagacccaggccatagtctggtcac
ctacgacaatagacacaccgggtgaatatcttgagaaccgctgcctgtacagtgcgctgctggccagcgcctgtgaagc
ggacattattgcgctggtactcaatgctgacgcgccgtggtcaccattttctcccggttttaccggaccaatgaaccga
cccgttattggcctcgttaccaaagccgatttagccaacccgcagcgtatttctcttgtggagagttggttagctcagg
ctggtgcgcaaaaagtatttgtcaccagcgcgctggagaataccggggtcgatgagatgttcatttttctgaatgcaaa
ggaatcttcctgtctcacaaaataa