BBa_K371019
type: ComponentDefinition
persistent identity: BBa K371019
display id: BBa_K371019
version: 1
was generated by: iGEM to SBOL conversion
description: pdu(Propanediol utilization)BMC shell gene under Plac control
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:16Z
mutable provenance: Citrobactor freudii, the strain was bought from NITE Biological Resource Center (NBRC). NBRC NO. is 12681. History:IFO 12681 <- Ajinomoto Co., Inc. (AJ 2619) <- ATCC 8090 <- C.H. Werkm
owned by: james
owned by: myers
mutable description: This composite consists of eight pdu bacterial microcompartment(BMC) structure gene following a natural lac promotor(BBa_R0010). These genes come from four basic parts,pduAB,pduJK,pduN and pduTUV, which consist of the natural RBS and gene separately.
discontinued: false
dominant: false
experience: experience/Works
group u list: _498_
m user id: 0
owner id: 3984
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Promotor BBa_R0010 was chosen to give a mediate transcription efficiency after induced by IPTG.
star: false
creator: Yang Zhang, Ruijun Zhu
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K371001
component: BBa_K371005
component: BBa_K371004
component: BBa_R0010
component: BBa_K371000
sequence annotation: BBa_R0010
sequence annotation: BBa_K371001
sequence annotation: BBa_K371000
sequence annotation: BBa_K371004
sequence annotation: BBa_K371005
sequence: https://synbiohub.org/public/igem/BBa_K371019_sequence/1

BBa_K371001
type: Component
persistent identity: component2084890
display id: component2084890
version: 1
top level: BBa_K371019
definition: BBa_K371001
access: public

BBa_K371005
type: Component
persistent identity: component2084917
display id: component2084917
version: 1
top level: BBa_K371019
definition: BBa_K371005
access: public

BBa_K371004
type: Component
persistent identity: component2084904
display id: component2084904
version: 1
top level: BBa_K371019
definition: BBa_K371004
access: public

BBa_R0010
type: Component
persistent identity: component2084875
display id: component2084875
version: 1
top level: BBa_K371019
definition: LacI
access: public

BBa_K371000
type: Component
persistent identity: component2084899
display id: component2084899
version: 1
top level: BBa_K371019
definition: BBa_K371000
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2084875
display id: annotation2084875
version: 1
top level: BBa_K371019
location: https://synbiohub.org/public/igem/BBa_K371019/annotation2084875/range2084875/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K371019/annotation2084875/range2084875/1
type: Range
persistent identity: range2084875
display id: range2084875
version: 1
top level: BBa_K371019
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K371001
type: SequenceAnnotation
persistent identity: annotation2084890
display id: annotation2084890
version: 1
top level: BBa_K371019
location: https://synbiohub.org/public/igem/BBa_K371019/annotation2084890/range2084890/1
component: BBa_K371001

https://synbiohub.org/public/igem/BBa_K371019/annotation2084890/range2084890/1
type: Range
persistent identity: range2084890
display id: range2084890
version: 1
top level: BBa_K371019
direction: direction/reverse
start: 209
end: 1320
orientation: inline

BBa_K371000
type: SequenceAnnotation
persistent identity: annotation2084899
display id: annotation2084899
version: 1
top level: BBa_K371019
location: https://synbiohub.org/public/igem/BBa_K371019/annotation2084899/range2084899/1
component: BBa_K371000

https://synbiohub.org/public/igem/BBa_K371019/annotation2084899/range2084899/1
type: Range
persistent identity: range2084899
display id: range2084899
version: 1
top level: BBa_K371019
direction: direction/reverse
start: 1329
end: 2111
orientation: inline

BBa_K371004
type: SequenceAnnotation
persistent identity: annotation2084904
display id: annotation2084904
version: 1
top level: BBa_K371019
location: https://synbiohub.org/public/igem/BBa_K371019/annotation2084904/range2084904/1
component: BBa_K371004

https://synbiohub.org/public/igem/BBa_K371019/annotation2084904/range2084904/1
type: Range
persistent identity: range2084904
display id: range2084904
version: 1
top level: BBa_K371019
direction: direction/reverse
start: 2120
end: 2413
orientation: inline

BBa_K371005
type: SequenceAnnotation
persistent identity: annotation2084917
display id: annotation2084917
version: 1
top level: BBa_K371019
location: https://synbiohub.org/public/igem/BBa_K371019/annotation2084917/range2084917/1
component: BBa_K371005

https://synbiohub.org/public/igem/BBa_K371019/annotation2084917/range2084917/1
type: Range
persistent identity: range2084917
display id: range2084917
version: 1
top level: BBa_K371019
direction: direction/reverse
start: 2422
end: 3817
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_K371004
type: ComponentDefinition
persistent identity: BBa K371004
display id: BBa_K371004
version: 1
was generated by: iGEM to SBOL conversion
description: pduN(Propanediol utilization gene N)[RBS+pduN]
created: 2010-08-26T11:00:00Z
modified: 2015-05-08T01:12:15Z
mutable provenance: pduJK come from Citrobacter Freundii genenom(NBRC NO.12681)
owned by: james
owned by: myers
mutable description: Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes, which belongs to a kind of BMC(bacterial microcompartment).Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduN consist of pdu N wich their natural RBS, which got from Citrobacter Freundii by PCR. PduN, together with others struture part of pdu operon, can be used to construct a empty proteinous microcompartment in bacteria.
discontinued: false
dominant: false
experience: experience/Works
group u list: _498_
m user id: 0
owner id: 3984
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: There are some silent mutation in sequencing result of pduN.
star: false
creator: Yang Zhang
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: RBS
sequence annotation: start codon
sequence annotation: pduN conding sequence
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_K371004_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2078724
display id: annotation2078724
version: 1
top level: BBa_K371004
location: https://synbiohub.org/public/igem/BBa_K371004/annotation2078724/range2078724/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K371004/annotation2078724/range2078724/1
type: Range
persistent identity: range2078724
display id: range2078724
version: 1
top level: BBa_K371004
direction: direction/forward
start: 4
end: 12
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078723
display id: annotation2078723
version: 1
top level: BBa_K371004
location: https://synbiohub.org/public/igem/BBa_K371004/annotation2078723/range2078723/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K371004/annotation2078723/range2078723/1
type: Range
persistent identity: range2078723
display id: range2078723
version: 1
top level: BBa_K371004
direction: direction/forward
start: 19
end: 21
orientation: inline

pduN conding sequence
type: SequenceAnnotation
persistent identity: annotation2078725
display id: annotation2078725
version: 1
top level: BBa_K371004
location: https://synbiohub.org/public/igem/BBa_K371004/annotation2078725/range2078725/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K371004/annotation2078725/range2078725/1
type: Range
persistent identity: range2078725
display id: range2078725
version: 1
top level: BBa_K371004
direction: direction/forward
start: 19
end: 294
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078722
display id: annotation2078722
version: 1
top level: BBa_K371004
location: https://synbiohub.org/public/igem/BBa_K371004/annotation2078722/range2078722/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K371004/annotation2078722/range2078722/1
type: Range
persistent identity: range2078722
display id: range2078722
version: 1
top level: BBa_K371004
direction: direction/forward
start: 292
end: 294
orientation: inline

BBa_K371005
type: ComponentDefinition
persistent identity: BBa K371005
display id: BBa_K371005
version: 1
was generated by: iGEM to SBOL conversion
description: pduTUV(Propanediol utilization gene T+U+V)[RBS+pduT+RBS+pduU+RBS+pduV]
created: 2010-08-26T11:00:00Z
modified: 2015-05-08T01:12:16Z
mutable provenance: pduJK come from Citrobacter Freundii genenom(NBRC NO.12681)
owned by: james
owned by: myers
mutable description: Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes, which belongs to a kind of BMC(bacterial microcompartment).Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduTUV consist of pduT, pduU and pduV wich their corresponding natural RBS, which got from Citrobacter Freundii by PCR. PduTUV, together with other struture parts of pdu operon, can be used to construct a empty proteinous microcompartment in bacteria.
discontinued: false
dominant: false
experience: experience/Works
group u list: _498_
m user id: 0
owner id: 3984
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Sequencing result shows that there are several silent mutation in pduT and pduU. PduV has some error mutation. We are still working on the function assay to certify whether the mutation pduV still function well.
star: false
creator: Yang Zhang
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: RBS
sequence annotation: pduT coding sequence
sequence annotation: start codon
sequence annotation: stop codon
sequence annotation: pduU coding sequence
sequence annotation: pduV coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K371005_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2078729
display id: annotation2078729
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078729/range2078729/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K371005/annotation2078729/range2078729/1
type: Range
persistent identity: range2078729
display id: range2078729
version: 1
top level: BBa_K371005
direction: direction/forward
start: 23
end: 28
orientation: inline

pduT coding sequence
type: SequenceAnnotation
persistent identity: annotation2078737
display id: annotation2078737
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078737/range2078737/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K371005/annotation2078737/range2078737/1
type: Range
persistent identity: range2078737
display id: range2078737
version: 1
top level: BBa_K371005
direction: direction/forward
start: 35
end: 589
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078732
display id: annotation2078732
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078732/range2078732/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K371005/annotation2078732/range2078732/1
type: Range
persistent identity: range2078732
display id: range2078732
version: 1
top level: BBa_K371005
direction: direction/forward
start: 35
end: 47
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2078730
display id: annotation2078730
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078730/range2078730/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K371005/annotation2078730/range2078730/1
type: Range
persistent identity: range2078730
display id: range2078730
version: 1
top level: BBa_K371005
direction: direction/forward
start: 576
end: 582
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078735
display id: annotation2078735
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078735/range2078735/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K371005/annotation2078735/range2078735/1
type: Range
persistent identity: range2078735
display id: range2078735
version: 1
top level: BBa_K371005
direction: direction/forward
start: 587
end: 589
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078733
display id: annotation2078733
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078733/range2078733/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K371005/annotation2078733/range2078733/1
type: Range
persistent identity: range2078733
display id: range2078733
version: 1
top level: BBa_K371005
direction: direction/forward
start: 589
end: 591
orientation: inline

pduU coding sequence
type: SequenceAnnotation
persistent identity: annotation2078727
display id: annotation2078727
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078727/range2078727/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K371005/annotation2078727/range2078727/1
type: Range
persistent identity: range2078727
display id: range2078727
version: 1
top level: BBa_K371005
direction: direction/forward
start: 589
end: 939
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2078731
display id: annotation2078731
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078731/range2078731/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K371005/annotation2078731/range2078731/1
type: Range
persistent identity: range2078731
display id: range2078731
version: 1
top level: BBa_K371005
direction: direction/forward
start: 931
end: 937
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078736
display id: annotation2078736
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078736/range2078736/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K371005/annotation2078736/range2078736/1
type: Range
persistent identity: range2078736
display id: range2078736
version: 1
top level: BBa_K371005
direction: direction/forward
start: 937
end: 939
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078734
display id: annotation2078734
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078734/range2078734/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K371005/annotation2078734/range2078734/1
type: Range
persistent identity: range2078734
display id: range2078734
version: 1
top level: BBa_K371005
direction: direction/forward
start: 944
end: 946
orientation: inline

pduV coding sequence
type: SequenceAnnotation
persistent identity: annotation2078728
display id: annotation2078728
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078728/range2078728/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K371005/annotation2078728/range2078728/1
type: Range
persistent identity: range2078728
display id: range2078728
version: 1
top level: BBa_K371005
direction: direction/forward
start: 944
end: 1396
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078726
display id: annotation2078726
version: 1
top level: BBa_K371005
location: https://synbiohub.org/public/igem/BBa_K371005/annotation2078726/range2078726/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K371005/annotation2078726/range2078726/1
type: Range
persistent identity: range2078726
display id: range2078726
version: 1
top level: BBa_K371005
direction: direction/forward
start: 1393
end: 1396
orientation: inline

BBa_K371000
type: ComponentDefinition
persistent identity: BBa K371000
display id: BBa_K371000
version: 1
was generated by: iGEM to SBOL conversion
description: pduJK(Propanediol utilization gene J+K)[RBS+pduJ+RBS+pduK]
created: 2010-08-26T11:00:00Z
modified: 2015-05-08T01:12:15Z
mutable provenance: pduAb come from Citrobacter Freundii genenom(NBRC NO.12681)
owned by: james
owned by: myers
mutable description: Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes. Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduAB consist of pdu A and pdu B wich their natural RBS, which got from Citrobacter Freundii by PCR. PduAB, together with others struture part of pdu operon, can be used to construct a empty proteinous microcompartment in bacteria.
discontinued: false
dominant: false
experience: experience/Works
group u list: _498_
m user id: 0
owner id: 3984
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The sequence results of pduAB, which we got by PCR from Citrobacter Freundii(NBRC NO.12681), are different from the sequence we found on the papar. We suspect that the strain we used is not same with the papar.
star: false
creator: Yang Zhang
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: pduJ RBS
sequence annotation: start codon
sequence annotation: pduJ coding sequence
sequence annotation: stop codon
sequence annotation: pduK RBS
sequence annotation: pduK coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K371000_sequence/1

pduJ RBS
type: SequenceAnnotation
persistent identity: annotation2078670
display id: annotation2078670
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078670/range2078670/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K371000/annotation2078670/range2078670/1
type: Range
persistent identity: range2078670
display id: range2078670
version: 1
top level: BBa_K371000
direction: direction/forward
start: 5
end: 12
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078671
display id: annotation2078671
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078671/range2078671/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K371000/annotation2078671/range2078671/1
type: Range
persistent identity: range2078671
display id: range2078671
version: 1
top level: BBa_K371000
direction: direction/forward
start: 19
end: 21
orientation: inline

pduJ coding sequence
type: SequenceAnnotation
persistent identity: annotation2078672
display id: annotation2078672
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078672/range2078672/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K371000/annotation2078672/range2078672/1
type: Range
persistent identity: range2078672
display id: range2078672
version: 1
top level: BBa_K371000
direction: direction/forward
start: 19
end: 294
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078674
display id: annotation2078674
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078674/range2078674/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K371000/annotation2078674/range2078674/1
type: Range
persistent identity: range2078674
display id: range2078674
version: 1
top level: BBa_K371000
direction: direction/forward
start: 292
end: 294
orientation: inline

pduK RBS
type: SequenceAnnotation
persistent identity: annotation2078675
display id: annotation2078675
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078675/range2078675/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K371000/annotation2078675/range2078675/1
type: Range
persistent identity: range2078675
display id: range2078675
version: 1
top level: BBa_K371000
direction: direction/forward
start: 307
end: 312
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078676
display id: annotation2078676
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078676/range2078676/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K371000/annotation2078676/range2078676/1
type: Range
persistent identity: range2078676
display id: range2078676
version: 1
top level: BBa_K371000
direction: direction/forward
start: 319
end: 321
orientation: inline

pduK coding sequence
type: SequenceAnnotation
persistent identity: annotation2078673
display id: annotation2078673
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078673/range2078673/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K371000/annotation2078673/range2078673/1
type: Range
persistent identity: range2078673
display id: range2078673
version: 1
top level: BBa_K371000
direction: direction/forward
start: 319
end: 783
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078677
display id: annotation2078677
version: 1
top level: BBa_K371000
location: https://synbiohub.org/public/igem/BBa_K371000/annotation2078677/range2078677/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K371000/annotation2078677/range2078677/1
type: Range
persistent identity: range2078677
display id: range2078677
version: 1
top level: BBa_K371000
direction: direction/forward
start: 781
end: 783
orientation: inline

BBa_K371001
type: ComponentDefinition
persistent identity: BBa K371001
display id: BBa_K371001
version: 1
was generated by: iGEM to SBOL conversion
description: pduAB(Propanediol utilization gene A+B)[RBS+pduA+RBS+pduB]
created: 2010-08-26T11:00:00Z
modified: 2015-05-08T01:12:15Z
mutable provenance: pduJK come from Citrobacter Freundii genenom(NBRC NO.12681)
owned by: james
owned by: myers
mutable description: Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes. Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduAB consist of pdu A and pdu B wich their corresponding natural RBS, which got from Citrobacter Freundii by PCR. PduAB, together with others struture part of pdu operon such as pdu JK, can be used to construct a empty proteinous microcompartment in bacteria.
discontinued: false
dominant: false
experience: experience/Works
group u list: _498_
m user id: 0
owner id: 3984
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The sequencing result show that the gene of pduAB has some silent mutations, which have no effect on the function of protein.
star: false
creator: Yang Zhang
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: start codon
sequence annotation: pduA coding sequence
sequence annotation: stop codon
sequence annotation: pduB coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K371001_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2078678
display id: annotation2078678
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078678/range2078678/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K371001/annotation2078678/range2078678/1
type: Range
persistent identity: range2078678
display id: range2078678
version: 1
top level: BBa_K371001
direction: direction/forward
start: 6
end: 11
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078685
display id: annotation2078685
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078685/range2078685/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K371001/annotation2078685/range2078685/1
type: Range
persistent identity: range2078685
display id: range2078685
version: 1
top level: BBa_K371001
direction: direction/forward
start: 19
end: 21
orientation: inline

pduA coding sequence
type: SequenceAnnotation
persistent identity: annotation2078679
display id: annotation2078679
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078679/range2078679/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K371001/annotation2078679/range2078679/1
type: Range
persistent identity: range2078679
display id: range2078679
version: 1
top level: BBa_K371001
direction: direction/forward
start: 19
end: 300
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2078683
display id: annotation2078683
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078683/range2078683/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K371001/annotation2078683/range2078683/1
type: Range
persistent identity: range2078683
display id: range2078683
version: 1
top level: BBa_K371001
direction: direction/forward
start: 285
end: 292
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078681
display id: annotation2078681
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078681/range2078681/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K371001/annotation2078681/range2078681/1
type: Range
persistent identity: range2078681
display id: range2078681
version: 1
top level: BBa_K371001
direction: direction/forward
start: 298
end: 300
orientation: inline

pduB coding sequence
type: SequenceAnnotation
persistent identity: annotation2078682
display id: annotation2078682
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078682/range2078682/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K371001/annotation2078682/range2078682/1
type: Range
persistent identity: range2078682
display id: range2078682
version: 1
top level: BBa_K371001
direction: direction/forward
start: 300
end: 1112
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2078680
display id: annotation2078680
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078680/range2078680/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K371001/annotation2078680/range2078680/1
type: Range
persistent identity: range2078680
display id: range2078680
version: 1
top level: BBa_K371001
direction: direction/forward
start: 300
end: 302
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2078684
display id: annotation2078684
version: 1
top level: BBa_K371001
location: https://synbiohub.org/public/igem/BBa_K371001/annotation2078684/range2078684/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K371001/annotation2078684/range2078684/1
type: Range
persistent identity: range2078684
display id: range2078684
version: 1
top level: BBa_K371001
direction: direction/forward
start: 1110
end: 1112
orientation: inline

https://synbiohub.org/public/igem/BBa_K371019_sequence/1
type: Sequence
persistent identity: BBa K371019 sequence
display id: BBa_K371019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaacccaggcgaggtctttatgcaacaagaagcgttaggaatggtagaaaccaaaggcttgacagcagccatagaggccgcagatgcaatggtgaagtcagccaatgtaatgctggtcggctacgaaaaaattggttcggggctggtaacagttattgtccgcggcgatgtcggcgcagtcaaagcagcaacagatgccggtgccgccgcagcccgtaatgtgggagaagtgaaagccgtacacgttatcccacgcccgcataccgatgtagaaaaaatcttaccgaagggaattagctaatgagcagcaatgaactggttgaacagatcatggcgcaggtgattgctcgcgtggcaacgccggaacagcaggctatcccagaaaataatcacccaacacgagaaacggctatggcagagaaaagctgtagtttaacggagtttgtcggtaccgcgattggcgacaccgtcggtctggtaatcgccaacgtagacagtgccctactggacgcaatgaaacttgaaaaacgctatcgctccattggcatccttggcgcccgtactggtgcgggtccacacatcatggccgctgatgaagcggtgaaagccaccaatactgaagtcgtcagtattgagttaccacgtgataccaaaggcggcgcaggtcatggctcgctgattattcttggcggcaacgatgtttccgacgtgaaacgcggcattgaagtcgcactgaaagagctggatcgcacctttggcgacgtttatgccaacgaagccggtcacatcgaaatgcaatacaccgcacgcgccagctacgcacttgaaaaagcctttggcgcaccgattggtcgtgcctgtggcgtcatcgtcggcgcaccagcatccgttggtgtcctgatggctgatactgccctgaaatccgccaacgtggaagttgtggcttacagctcccctgctcatggcaccagcttcagtaacgaagccattctggttatttcgggtgactctggcgctgttcgtcaggctgttatctctgcccgtgaaatcggtaaaaccgtactcgggaccctcggctcagagccgaaaaacgatcgtccgtcctacatctgatactagagccacaggagaaaagcagtatgaataacgcactgggactggttgaaacaaaagggcttgtcggcgctattgaagccgctgatgccatggtgaaatccgcaaacgtgcagttggttggttacgaaaaaatcggctctggcctgatcaacgttatggttcgcggtgatgtcggcgcggtgaaagcagccgtagatgctggaagcgcagcagcaagcgccgttggtgaggtgaaatcctgccacgttatcccgcgtccgcacagcgacgttgaagccattttacctaaatccgcataagtcgttgcaaccaaggagcacagcgtgaagcaatcactgggattacttgaagttagtggtctggcattagccatcagttgcgcggacgtcatggcgaaagccgcctccatcacgctggtgggcctcgaaaaaaccaacggctcaggctggacggtgatcaagataatcggggatgtggcctccgtccaggcggccatttccactggtgtcagtttcgctgaccagcgagatggactggtggctcacaaagtcatttccagacccggggacgggatcctgtcgcatagcgttgtcctggagcctgaaccaacgcccgaccccataccagccataccacatgaagaaatccctgaggaccatgcggcacccgaagcaccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371004_sequence/1
type: Sequence
persistent identity: BBa K371004 sequence
display id: BBa_K371004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaagcaggagtgagctatgcatctggcacgggttacaggcgttgtggtttccacgcaaaaatctccatcactggtgggaaaaaaactgttgctggtacgtcgggtaagtgctgacggagagcttcctgcgtctccagtgagtggagatgaagtcgctgttgattctgtcggcgcggggaccggcgaactggtattactcagcagcgggtccagcgccagacacgttttttccggcccgaatgaggccattgatctggctatcgtcggcattgtcgacacgctttctcgttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371005_sequence/1
type: Sequence
persistent identity: BBa K371005 sequence
display id: BBa_K371005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgagcaatccatcacggtaataagaggttaatcatgtctcaggctatagggattttagaactcaccagcattgccaaaggaatggaagctggcgatgccatgttaaaaagcgcgaatgtgaatttactggtcagcaagaccatctgcccgggaaaatttctactcatgctcggtggtgacgtaggtgccgtacaacaggcgattgccactggaacatctcttgctggcgatatgctcgttgatagtctggtactccccaacattcatgccagcgtactacccgcaatcagcggactaaacagcgtagataagcgtcaggcagttggtattgtcgaaacatggagcgttgctgcctgcatctgcgccgccgaccgggcggttaaagcctccaacgtcacgctggtccgcgtccgtatggcatttggtattggcggcaagtgttacatggtcgtagctggcgatgtatccgatgtgaataacgcggtgacggttgccagtgaaagtgcgggtgaaaaaggcctgctggtttaccgctcggtcatcccccgtccacacgaaagcatgtggcgacaaatggtggaaggataatggaaagacaacccaccacggatcgtatgattcaggaatatgttcctggcaaacaggtcacgttggcgcaccttatcgccaatcccggtaaggatttgttcaagaaactgggattacctgattcggtttctgcgatcggtattctgaccattacccctagcgaagcctcaatcatcgcctgtgatatcgccacgaaatcaggggcggtagagattggttttctcgaccggtttaccggcgcggtggtactgacgggcgatgtttcagccgttgagtacgcgctgaaacaggtaacccgaacgctgggcgaaatgatgcgttttaccgcgtgtcccatcacccggacgtaatctcgtgaaacgtatcatgctaattggccccagccagtgcggtaaaacgtcgctcacacagtgcatgcgcggagaggtgctccactatcagaagacccaggccatagtctggtcacctacgacaatagacacaccgggtgaatatcttgagaaccgctgcctgtacagtgcgctgctggccagcgcctgtgaagcggacattattgcgctggtactcaatgctgacgcgccgtggtcaccattttctcccggttttaccggaccaatgaaccgacccgttattggcctcgttaccaaagccgatttagccaacccgcagcgtatttctcttgtggagagttggttagctcaggctggtgcgcaaaaagtatttgtcaccagcgcgctggagaataccggggtcgatgagatgttcatttttctgaatgcaaaggaatcttcctgtctcacaaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371000_sequence/1
type: Sequence
persistent identity: BBa K371000 sequence
display id: BBa_K371000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaggagaaaagcagtatgaataacgcactgggactggttgaaacaaaagggcttgtcggcgctattgaagccgctgatgccatggtgaaatccgcaaacgtgcagttggttggttacgaaaaaatcggctctggcctgatcaacgttatggttcgcggtgatgtcggcgcggtgaaagcagccgtagatgctggaagcgcagcagcaagcgccgttggtgaggtgaaatcctgccacgttatcccgcgtccgcacagcgacgttgaagccattttacctaaatccgcataagtcgttgcaaccaaggagcacagcgtgaagcaatcactgggattacttgaagttagtggtctggcattagccatcagttgcgcggacgtcatggcgaaagccgcctccatcacgctggtgggcctcgaaaaaaccaacggctcaggctggacggtgatcaagataatcggggatgtggcctccgtccaggcggccatttccactggtgtcagtttcgctgaccagcgagatggactggtggctcacaaagtcatttccagacccggggacgggatcctgtcgcatagcgttgtcctggagcctgaaccaacgcccgaccccataccagccataccacatgaagaaatccctgaggaccatgcggcacccgaagcaccgcaagatgcagaattgattagctgcaatttgtgtcttgatcctgcctgccctcgccaaaaaggtgagccgcgcacactttgtcttcactccggcaaacgaggtgaagcgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371001_sequence/1
type: Sequence
persistent identity: BBa K371001 sequence
display id: BBa_K371001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacccaggcgaggtctttatgcaacaagaagcgttaggaatggtagaaaccaaaggcttgacagcagccatagaggccgcagatgcaatggtgaagtcagccaatgtaatgctggtcggctacgaaaaaattggttcggggctggtaacagttattgtccgcggcgatgtcggcgcagtcaaagcagcaacagatgccggtgccgccgcagcccgtaatgtgggagaagtgaaagccgtacacgttatcccacgcccgcataccgatgtagaaaaaatcttaccgaagggaattagctaatgagcagcaatgaactggttgaacagatcatggcgcaggtgattgctcgcgtggcaacgccggaacagcaggctatcccagaaaataatcacccaacacgagaaacggctatggcagagaaaagctgtagtttaacggagtttgtcggtaccgcgattggcgacaccgtcggtctggtaatcgccaacgtagacagtgccctactggacgcaatgaaacttgaaaaacgctatcgctccattggcatccttggcgcccgtactggtgcgggtccacacatcatggccgctgatgaagcggtgaaagccaccaatactgaagtcgtcagtattgagttaccacgtgataccaaaggcggcgcaggtcatggctcgctgattattcttggcggcaacgatgtttccgacgtgaaacgcggcattgaagtcgcactgaaagagctggatcgcacctttggcgacgtttatgccaacgaagccggtcacatcgaaatgcaatacaccgcacgcgccagctacgcacttgaaaaagcctttggcgcaccgattggtcgtgcctgtggcgtcatcgtcggcgcaccagcatccgttggtgtcctgatggctgatactgccctgaaatccgccaacgtggaagttgtggcttacagctcccctgctcatggcaccagcttcagtaacgaagccattctggttatttcgggtgactctggcgctgttcgtcaggctgttatctctgcccgtgaaatcggtaaaaccgtactcgggaccctcggctcagagccgaaaaacgatcgtccgtcctacatctga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z