BBa_K371025 1 BBa_K371025 TAATAA[intermediate] 2010-10-26T11:00:00Z 2015-05-08T01:12:16Z ~~~ This part consists of two stop codon taa. It is a intermidate part use to construct a complete fusion protein. For more disign consideration, please go to BBa_K371030 or BBa_K371031 false false _498_ 0 3984 9 Not in stock false ~~~ false Yang Zhang annotation2109483 1 stop codon range2109483 1 1 6 BBa_K371063 1 BBa_K371063 pduA+RBS+pduB 2010-11-05T12:00:00Z 2015-05-08T01:12:16Z MetaFusion pduA+RBS+pduB false false _498_ 0 3908 9 Not in stock false pduA+RBS+pduB false Hao Jiang component2373201 1 BBa_K371040 component2373198 1 BBa_B0034 component2373196 1 BBa_K371033 annotation2373201 1 BBa_K371040 range2373201 1 328 1140 annotation2373196 1 BBa_K371033 range2373196 1 1 301 annotation2373198 1 BBa_B0034 range2373198 1 310 321 BBa_K371033 1 BBa_K371033 pduA+TAATAA 2010-10-26T11:00:00Z 2015-05-08T01:12:16Z Citrobactor freudii, the strain was bought from NITE Biological Resource Center (NBRC). NBRC NO. is 12681. History:IFO 12681 <- Ajinomoto Co., Inc. (AJ 2619) <- ATCC 8090 <- C.H. Werkman This is a intermediate part. To see the complete part go to BBa_K371032 false false _498_ 0 3984 9 Not in stock false .. false Yang Zhang component2372989 1 BBa_K371008 component2372991 1 BBa_K371025 annotation2372991 1 BBa_K371025 range2372991 1 296 301 annotation2372989 1 BBa_K371008 range2372989 1 1 295 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K371050 1 BBa_K371050 MSF(meta-suffix) 2010-10-26T11:00:00Z 2015-05-08T01:12:16Z direct synthesis This part is a extention of BioBrick prefix. It is used in noval BioBrick assemble standard BBF RFC 53. false false _498_ 0 3984 9 Not in stock true BBF RFC53 false Yang Zhang annotation2110071 1 stop codon range2110071 1 1 3 annotation2105196 1 BglII range2105196 1 6 10 annotation2105197 1 EarI range2105197 1 2 7 annotation2105198 1 MSF range2105198 1 1 10 BBa_K371008 1 BBa_K371008 pduA 2010-10-07T11:00:00Z 2015-05-08T01:12:16Z Citrobactor freudii, the strain was bought from NITE Biological Resource Center (NBRC). NBRC NO. is 12681. History:IFO 12681 <- Ajinomoto Co., Inc. (AJ 2619) <- ATCC 8090 <- C.H. Werkman This part encodes pdu A, which is a shell protein of Citrobactor freundii. false false _498_ 0 3984 9 It's complicated true In order to isolate pduA from the Citrobactor freundii genome, following primers has been designed to make it a standard Biobrick. false Yang Zhang annotation2247243 1 pdu A range2247243 1 1 295 BBa_K371040 1 BBa_K371040 pduB 2010-10-26T11:00:00Z 2015-05-08T01:12:16Z Citrobactor freudii, the strain was bought from NITE Biological Resource Center (NBRC). NBRC NO. is 12681. History:IFO 12681 <- Ajinomoto Co., Inc. (AJ 2619) <- ATCC 8090 <- C.H. Werkm This part encodes pdu A, which is a shell protein of pdu Bacterial Microcompartment in Citrobactor freundii false false _498_ 0 3984 9 Not in stock false .. false Yang Zhang annotation2113928 1 pduB coding sequence range2113928 1 1 813 annotation2113929 1 start codon range2113929 1 1 3 BBa_K371032 1 BBa_K371032 MPF(meta-prefix)+pduA+RBS+pduB+MSF(meta-suffix) 2010-10-26T11:00:00Z 2015-05-08T01:12:16Z Citrobactor freudii, the strain was bought from NITE Biological Resource Center (NBRC). NBRC NO. is 12681. History:IFO 12681 <- Ajinomoto Co., Inc. (AJ 2619) <- ATCC 8090 <- C.H. Werkman This part consist of pduA, pduB and RBS BBa_B0034. Pdu A and pdu B belong to shell protein of pdu BMC(baterial microcompartment). BMC is a large proteineious complex which shell consist of at least five different kind protein. These proterins share the same protein doman named BMC domain. false false _498_ 0 3984 9 Not in stock true this part is compatible with BBF RFC 53 standard false Yang Zhang component2373650 1 BBa_K371063 component2373655 1 BBa_K371050 component2373639 1 BBa_K371049 annotation2373639 1 BBa_K371049 range2373639 1 1 10 annotation2373655 1 BBa_K371050 range2373655 1 1151 1160 annotation2373650 1 BBa_K371063 range2373650 1 11 1150 BBa_K371049 1 BBa_K371049 MPF(meta-prefix) 2010-10-26T11:00:00Z 2015-05-08T01:12:16Z direct synthesis This part is a extention of BioBrick prefix. It is used in noval BioBrick assemble standard BBF RFC 53. false false _498_ 0 3984 9 Not in stock true BBF RFC53 false Yang Zhang annotation2105193 1 meta-prefix range2105193 1 1 10 annotation2105194 1 SacI range2105194 1 1 6 annotation2105195 1 EarI range2105195 1 4 9 annotation2105192 1 SapI range2105192 1 3 9 BBa_K371008_sequence 1 atgcaacaagaagcgttaggaatggtagaaaccaaaggcttgacagcagccatagaggccgcagatgcaatggtgaagtcagccaatgtaatgctggtcggctacgaaaaaattggttcggggctggtaacagttattgtccgcggcgatgtcggcgcagtcaaagcagcaacagatgccggtgccgccgcagcccgtaatgtgggagaagtgaaagccgtacacgttatcccacgcccgcataccgatgtagaaaaaatcttaccgaagggaattagcggttgaagagatctga BBa_B0034_sequence 1 aaagaggagaaa BBa_K371050_sequence 1 tgaagagatc BBa_K371033_sequence 1 atgcaacaagaagcgttaggaatggtagaaaccaaaggcttgacagcagccatagaggccgcagatgcaatggtgaagtcagccaatgtaatgctggtcggctacgaaaaaattggttcggggctggtaacagttattgtccgcggcgatgtcggcgcagtcaaagcagcaacagatgccggtgccgccgcagcccgtaatgtgggagaagtgaaagccgtacacgttatcccacgcccgcataccgatgtagaaaaaatcttaccgaagggaattagcggttgaagagatctgataataa BBa_K371040_sequence 1 atgagcagcaatgaactggttgaacagatcatggcgcaggtgattgctcgcgtggcaacgccggaacagcaggctatcccagaaaataatcacccaacacgagaaacggctatggcagagaaaagctgtagtttaacggagtttgtcggtaccgcgattggcgacaccgtcggtctggtaatcgccaacgtagacagtgccctactggacgcaatgaaacttgaaaaacgctatcgctccattggcatccttggcgcccgtactggtgcgggtccacacatcatggccgctgatgaagcggtgaaagccaccaatactgaagtcgtcagtattgagttaccacgtgataccaaaggcggcgcaggtcatggctcgctgattattcttggcggcaacgatgtttccgacgtgaaacgcggcattgaagtcgcactgaaagagctggatcgcacctttggcgacgtttatgccaacgaagccggtcacatcgaaatgcaatacaccgcacgcgccagctacgcacttgaaaaagcctttggcgcaccgattggtcgtgcctgtggcgtcatcgtcggcgcaccagcatccgttggtgtcctgatggctgatactgccctgaaatccgccaacgtggaagttgtggcttacagctcccctgctcatggcaccagcttcagtaacgaagccattctggttatttcgggtgactctggcgctgttcgtcaggctgttatctctgcccgtgaaatcggtaaaaccgtactcgggaccctcggctcagagccgaaaaacgatcgtccgtcctacatcggt BBa_K371025_sequence 1 taataa BBa_K371063_sequence 1 atgcaacaagaagcgttaggaatggtagaaaccaaaggcttgacagcagccatagaggccgcagatgcaatggtgaagtcagccaatgtaatgctggtcggctacgaaaaaattggttcggggctggtaacagttattgtccgcggcgatgtcggcgcagtcaaagcagcaacagatgccggtgccgccgcagcccgtaatgtgggagaagtgaaagccgtacacgttatcccacgcccgcataccgatgtagaaaaaatcttaccgaagggaattagcggttgaagagatctgataataatactagagaaagaggagaaatactagatgagcagcaatgaactggttgaacagatcatggcgcaggtgattgctcgcgtggcaacgccggaacagcaggctatcccagaaaataatcacccaacacgagaaacggctatggcagagaaaagctgtagtttaacggagtttgtcggtaccgcgattggcgacaccgtcggtctggtaatcgccaacgtagacagtgccctactggacgcaatgaaacttgaaaaacgctatcgctccattggcatccttggcgcccgtactggtgcgggtccacacatcatggccgctgatgaagcggtgaaagccaccaatactgaagtcgtcagtattgagttaccacgtgataccaaaggcggcgcaggtcatggctcgctgattattcttggcggcaacgatgtttccgacgtgaaacgcggcattgaagtcgcactgaaagagctggatcgcacctttggcgacgtttatgccaacgaagccggtcacatcgaaatgcaatacaccgcacgcgccagctacgcacttgaaaaagcctttggcgcaccgattggtcgtgcctgtggcgtcatcgtcggcgcaccagcatccgttggtgtcctgatggctgatactgccctgaaatccgccaacgtggaagttgtggcttacagctcccctgctcatggcaccagcttcagtaacgaagccattctggttatttcgggtgactctggcgctgttcgtcaggctgttatctctgcccgtgaaatcggtaaaaccgtactcgggaccctcggctcagagccgaaaaacgatcgtccgtcctacatcggt BBa_K371032_sequence 1 gagctcttcaatgcaacaagaagcgttaggaatggtagaaaccaaaggcttgacagcagccatagaggccgcagatgcaatggtgaagtcagccaatgtaatgctggtcggctacgaaaaaattggttcggggctggtaacagttattgtccgcggcgatgtcggcgcagtcaaagcagcaacagatgccggtgccgccgcagcccgtaatgtgggagaagtgaaagccgtacacgttatcccacgcccgcataccgatgtagaaaaaatcttaccgaagggaattagcggttgaagagatctgataataatactagagaaagaggagaaatactagatgagcagcaatgaactggttgaacagatcatggcgcaggtgattgctcgcgtggcaacgccggaacagcaggctatcccagaaaataatcacccaacacgagaaacggctatggcagagaaaagctgtagtttaacggagtttgtcggtaccgcgattggcgacaccgtcggtctggtaatcgccaacgtagacagtgccctactggacgcaatgaaacttgaaaaacgctatcgctccattggcatccttggcgcccgtactggtgcgggtccacacatcatggccgctgatgaagcggtgaaagccaccaatactgaagtcgtcagtattgagttaccacgtgataccaaaggcggcgcaggtcatggctcgctgattattcttggcggcaacgatgtttccgacgtgaaacgcggcattgaagtcgcactgaaagagctggatcgcacctttggcgacgtttatgccaacgaagccggtcacatcgaaatgcaatacaccgcacgcgccagctacgcacttgaaaaagcctttggcgcaccgattggtcgtgcctgtggcgtcatcgtcggcgcaccagcatccgttggtgtcctgatggctgatactgccctgaaatccgccaacgtggaagttgtggcttacagctcccctgctcatggcaccagcttcagtaacgaagccattctggttatttcgggtgactctggcgctgttcgtcaggctgttatctctgcccgtgaaatcggtaaaaccgtactcgggaccctcggctcagagccgaaaaacgatcgtccgtcctacatcggttgaagagatc BBa_K371049_sequence 1 gagctcttca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z