BBa_K371049
1
BBa_K371049
MPF(meta-prefix)
2010-10-26T11:00:00Z
2015-05-08T01:12:16Z
direct synthesis
This part is a extention of BioBrick prefix. It is used in noval BioBrick assemble standard BBF RFC 53.
false
false
_498_
0
3984
9
Not in stock
true
BBF RFC53
false
Yang Zhang
annotation2105194
1
SacI
range2105194
1
1
6
annotation2105193
1
meta-prefix
range2105193
1
1
10
annotation2105192
1
SapI
range2105192
1
3
9
annotation2105195
1
EarI
range2105195
1
4
9
BBa_K371012
1
BBa_K371012
pduN
2010-10-08T11:00:00Z
2015-05-08T01:12:16Z
Citrobactor freudii, the strain was bought from NITE Biological Resource Center (NBRC). NBRC NO. is 12681. History:IFO 12681 <- Ajinomoto Co., Inc. (AJ 2619) <- ATCC 8090 <- C.H. Werkm
This part encodes pdu J proterin, which is a shell protein of Citrobactor freundii bacterial microcompartment(BMC) .
false
false
_498_
0
3984
9
It's complicated
true
In order to isolate pduA from the Citrobactor freundii genome, following primers has been designed to make it a standard Biobrick.
false
Yang Zhang
annotation2247341
1
pdu N
range2247341
1
1
289
BBa_K371050
1
BBa_K371050
MSF(meta-suffix)
2010-10-26T11:00:00Z
2015-05-08T01:12:16Z
direct synthesis
This part is a extention of BioBrick prefix. It is used in noval BioBrick assemble standard BBF RFC 53.
false
false
_498_
0
3984
9
Not in stock
true
BBF RFC53
false
Yang Zhang
annotation2105198
1
MSF
range2105198
1
1
10
annotation2105197
1
EarI
range2105197
1
2
7
annotation2110071
1
stop codon
range2110071
1
1
3
annotation2105196
1
BglII
range2105196
1
6
10
BBa_K371025
1
BBa_K371025
TAATAA[intermediate]
2010-10-26T11:00:00Z
2015-05-08T01:12:16Z
~~~
This part consists of two stop codon taa. It is a intermidate part use to construct a complete fusion protein. For more disign consideration, please go to BBa_K371030 or BBa_K371031
false
false
_498_
0
3984
9
Not in stock
false
~~~
false
Yang Zhang
annotation2109483
1
stop codon
range2109483
1
1
6
BBa_K371038
1
BBa_K371038
pduT
2010-10-26T11:00:00Z
2015-05-08T01:12:16Z
Citrobactor freudii, the strain was bought from NITE Biological Resource Center (NBRC). NBRC NO. is 12681. History:IFO 12681 <- Ajinomoto Co., Inc. (AJ 2619) <- ATCC 8090 <- C.H. Werkm
pduN with MPF and MSF at each end.
This part encodes pdu J proterin, which is a shell protein of Citrobactor freundii bacterial microcompartment(BMC) .
false
false
_498_
0
3984
9
Not in stock
false
..
false
Yang Zhang
annotation2112476
1
start codon
range2112476
1
1
3
annotation2112477
1
pduT coding sequence
range2112477
1
1
552
BBa_K371036
1
BBa_K371036
MPF(meta-prefix)+pduN+RBS+pduT+MSF(meta-suffix)
2010-10-26T11:00:00Z
2015-05-08T01:12:16Z
Citrobactor freudii, the strain was bought from NITE Biological Resource Center (NBRC). NBRC NO. is 12681. History:IFO 12681 <- Ajinomoto Co., Inc. (AJ 2619) <- ATCC 8090 <- C.H. Werkman
This part consist of pduN, pduT and RBS BBa_B0034. Pdu N and pdu T belong to shell protein of pdu BMC(baterial microcompartment). BMC is a large proteineious complex which are metabolically active struture that are bound by a proterinaceous shell. The shell of BMC consist of at least five different kind protein. These proterins share the same protein doman named BMC domain.
false
false
_498_
0
3984
9
It's complicated
true
this part is compatible with BBF RFC 53 standard
false
Yang Zhang
component2372915
1
BBa_K371049
component2372921
1
BBa_B0034
component2372917
1
BBa_K371012
component2372929
1
BBa_K371050
component2372924
1
BBa_K371038
component2372919
1
BBa_K371025
annotation2372915
1
BBa_K371049
range2372915
1
1
10
annotation2372929
1
BBa_K371050
range2372929
1
870
879
annotation2372924
1
BBa_K371038
range2372924
1
318
869
annotation2372921
1
BBa_B0034
range2372921
1
306
317
annotation2372917
1
BBa_K371012
range2372917
1
11
299
annotation2372919
1
BBa_K371025
range2372919
1
300
305
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K371038_sequence
1
atgtctcaggctatagggattttagaactcaccagcattgccaaaggaatggaagctggcgatgccatgttaaaaagcgcgaatgtgaatttactggtcagcaagaccatctgcccgggaaaatttctactcatgctcggtggtgacgtaggtgccgtacaacaggcgattgccactggaacatctcttgctggcgatatgctcgttgatagtctggtactccccaacattcatgccagcgtactacccgcaatcagcggactaaacagcgtagataagcgtcaggcagttggtattgtcgaaacatggagcgttgctgcctgcatctgcgccgccgaccgggcggttaaagcctccaacgtcacgctggtccgcgtccatatggcatttggtattggcggcaagtgttacatggtcgtagctggcgatgtatccgatgtgaataacgcggtgacggttgccagtgaaagtgcgggtgaaaaaggcctgctggtttaccgctcggtcatcccccgtccacacgaaagcatgtggcgacagatggtggagggt
BBa_K371036_sequence
1
gagctcttcaatgcatctggcacgggttacaggcgttgtggtttccacgcaaaaatctccatcactggtgggaaaaaaactgttgctggtacgtcgggtaagtgctgacggagagcttcctgcgtctccagtgagtggagatgaagtcgctgttgattctgtcggcgcggggaccggcgaactggtattactcagcagcgggtccagcgccagacacgttttttccggcccgaatgaggccattgatctggctatcgtcggcattgtcgacacgctttctcgtggttgaagagatctgataataaaaagaggagaaaatgtctcaggctatagggattttagaactcaccagcattgccaaaggaatggaagctggcgatgccatgttaaaaagcgcgaatgtgaatttactggtcagcaagaccatctgcccgggaaaatttctactcatgctcggtggtgacgtaggtgccgtacaacaggcgattgccactggaacatctcttgctggcgatatgctcgttgatagtctggtactccccaacattcatgccagcgtactacccgcaatcagcggactaaacagcgtagataagcgtcaggcagttggtattgtcgaaacatggagcgttgctgcctgcatctgcgccgccgaccgggcggttaaagcctccaacgtcacgctggtccgcgtccatatggcatttggtattggcggcaagtgttacatggtcgtagctggcgatgtatccgatgtgaataacgcggtgacggttgccagtgaaagtgcgggtgaaaaaggcctgctggtttaccgctcggtcatcccccgtccacacgaaagcatgtggcgacagatggtggagggttgaagagatc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K371050_sequence
1
tgaagagatc
BBa_K371025_sequence
1
taataa
BBa_K371049_sequence
1
gagctcttca
BBa_K371012_sequence
1
atgcatctggcacgggttacaggcgttgtggtttccacgcaaaaatctccatcactggtgggaaaaaaactgttgctggtacgtcgggtaagtgctgacggagagcttcctgcgtctccagtgagtggagatgaagtcgctgttgattctgtcggcgcggggaccggcgaactggtattactcagcagcgggtccagcgccagacacgttttttccggcccgaatgaggccattgatctggctatcgtcggcattgtcgacacgctttctcgtggttgaagagatctga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z