##gff-version 3
##sequence-region BBa_K371051 1 2072
BBa_K371051	.	sequence_feature	2051	2072	.	+	0	ID=annotation2113455;Name=prefix
BBa_K371051	.	sequence_feature	1042	1147	.	+	0	ID=annotation2113447;Name=T0 terminator
BBa_K371051	.	sequence_feature	276	276	.	+	0	ID=annotation2113446;Name=ORI
BBa_K371051	.	sequence_feature	261	875	.	+	0	ID=annotation2113445;Name=rep (pMB1)
BBa_K371051	.	sequence_feature	1	21	.	+	0	ID=annotation2113440;Name=suffix
BBa_K371051	.	point_mutation	1149	1149	.	+	0	ID=annotation2113448;Name=C->G silent muation
BBa_K371051	.	stem_loop	22	93	.	+	0	ID=annotation2113441;Name=E. coli his operon terminator
BBa_K371051	.	sequence_alteration	34	35	.	+	0	ID=annotation2113443;Name=Deletion of AC
BBa_K371051	.	sequence_alteration	1919	1919	.	+	0	ID=annotation2113451;Name=Extra C discovered in sequencing
BBa_K371051	.	stem_loop	2022	2041	.	+	0	ID=annotation2113454;Name=Stem loop
BBa_K371051	.	CDS	1162	1821	.	+	0	ID=annotation2113449;Name=Chloramphenicol resistance
BBa_K371051	.	sequence_alteration	1939	1939	.	+	0	ID=annotation2113452;Name=C->T mutation
BBa_K371051	.	primer_binding_site	157	176	.	+	0	ID=annotation2113444;Name=Verification reverse (VR) primer binding site
BBa_K371051	.	point_mutation	1237	1237	.	+	0	ID=annotation2113450;Name=A->G silent mutation
BBa_K371051	.	stem_loop	30	64	.	+	0	ID=annotation2113442;Name=Stem loop
BBa_K371051	.	stem_loop	2009	2012	.	+	0	ID=annotation2113453;Name=end of terminator
>BBa_K371051
tactagtagcggccgctgcagtccggcaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaag
attacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagct
cactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaa
aggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcg
acgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgc
tctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagct
cacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccga
ccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccact
ggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacacta
gaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaa
acaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagat
cctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaa
aaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtc
tgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagt
tctgaggtcattactggatctatcaacaggagtccaagcgaggtcgatatcaaattacgccccgccctgccactcatcg
cagtactgttgtaattcattaagcattctgccgacatggaagccatcacagacggcatgatgaacctgaatcgccagcg
gcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccac
gtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaa
taggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcac
tccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctc
accgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaac
ttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaa
ctgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctc
cattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtga
aagttggaacctcttacgtgccccgatcaactcgagtgccacttgacgtctaagaaaccattattatcatgacattaac
ctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaat
tcgcggccgcttctagag