BBa_K371051 1 BBa_K371051 pSB1C5 (1bp mutation of pSB1C3 to remove SacI) 2010-10-26T11:00:00Z 2015-05-08T01:12:16Z this part comes from the partsregistry BBa_pSB1C3 This part is a plasmid bone, which has only a single site mutation compared with pSB1C3. The SacI site is mutated to allow this plasmid compatible to BBF EFC 53 standard. false false _498_ 0 7269 9 It's complicated true In order to make our working plasmid compatible to BBF RFC 53, we search the restriction enzymen site in pSB1C3 plasmid. And we found a SacI site in the pSB1C3. The site is between downstream of chloramphenicol and the T0 terminator. We think the mutation does not affect the regular function of pSB1C3 plasmid. So all of our following experiment of fusion protein was done in this new plasmid. false Ruijun Zhu annotation2113451 1 Extra C discovered in sequencing range2113451 1 1919 1919 annotation2113449 1 Chloramphenicol resistance range2113449 1 1162 1821 annotation2113452 1 C->T mutation range2113452 1 1939 1939 annotation2113454 1 Stem loop range2113454 1 2022 2041 annotation2113453 1 end of terminator range2113453 1 2009 2012 annotation2113442 1 Stem loop range2113442 1 30 64 annotation2113441 1 E. coli his operon terminator range2113441 1 22 93 annotation2113444 1 Verification reverse (VR) primer binding site range2113444 1 157 176 annotation2113447 1 T0 terminator range2113447 1 1042 1147 annotation2113450 1 A->G silent mutation range2113450 1 1237 1237 annotation2113446 1 ORI range2113446 1 276 276 annotation2113445 1 rep (pMB1) range2113445 1 261 875 annotation2113455 1 prefix range2113455 1 2051 2072 annotation2113443 1 Deletion of AC range2113443 1 34 35 annotation2113440 1 suffix range2113440 1 1 21 annotation2113448 1 C->G silent muation range2113448 1 1149 1149 BBa_K371051_sequence 1 tactagtagcggccgctgcagtccggcaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgaggtcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacagacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccccgatcaactcgagtgccacttgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z