LOCUS       BBa_K371052              422 bp    DNA     linear       22-Dec-2024
DEFINITION  RFC 53 easy expression biobrick
ACCESSION   BBa_K371052
VERSION     BBa_K371052.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    231..235
                     /label=EarI 
     misc_feature    238..243
                     /label=NheI
     misc_feature    244..250
                     /label=EarI
     misc_feature    227..285
                     /label=BBa_K371045
     stem_loop       305..348
                     /label=stem_loop
     misc_feature    294..373
                     /label=BBa_B0010
     terminator      294..373
                     /label=BBa_B0010
     stem_loop       389..408
                     /label=T7 TE
     misc_feature    415..415
                     /label=stop
     polyA_site      409..422
                     /label=polya
     misc_feature    382..422
                     /label=BBa_B0012
     terminator      382..422
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt gaagagcgct
      241 agcgctcttc aggttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca
      301 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      361 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      421 ta
//