BBa_K371049 1 BBa_K371049 MPF(meta-prefix) 2010-10-26T11:00:00Z 2015-05-08T01:12:16Z direct synthesis This part is a extention of BioBrick prefix. It is used in noval BioBrick assemble standard BBF RFC 53. false false _498_ 0 3984 9 Not in stock true BBF RFC53 false Yang Zhang annotation2105194 1 SacI range2105194 1 1 6 annotation2105195 1 EarI range2105195 1 4 9 annotation2105193 1 meta-prefix range2105193 1 1 10 annotation2105192 1 SapI range2105192 1 3 9 BBa_K371024 1 BBa_K371024 10*GS linker(for RFC 53 protein fusion) 2010-10-26T11:00:00Z 2015-05-08T01:12:16Z This part is not long, so it can be easily get by direct annealing two oligonucleotides. This part is a short amino sequence used to link or fuse two protein domain together to make a large chimera protein. false false _498_ 0 3984 9 It's complicated true different codons of Gly and Ser was choosed in order to ignore the mismatch between forward sequence and reverse sequence when they are annealing. false Yang Zhang annotation2109630 1 10*GS range2109630 1 1 30 BBa_K371050 1 BBa_K371050 MSF(meta-suffix) 2010-10-26T11:00:00Z 2015-05-08T01:12:16Z direct synthesis This part is a extention of BioBrick prefix. It is used in noval BioBrick assemble standard BBF RFC 53. false false _498_ 0 3984 9 Not in stock true BBF RFC53 false Yang Zhang annotation2105197 1 EarI range2105197 1 2 7 annotation2110071 1 stop codon range2110071 1 1 3 annotation2105196 1 BglII range2105196 1 6 10 annotation2105198 1 MSF range2105198 1 1 10 BBa_K371037 1 BBa_K371037 pduA 2010-10-26T11:00:00Z 2015-05-08T01:12:16Z .. This parts is a fusion protein between pduA protein and RFP protein(BBa_E1010). At each end is special DNA sequence that make this parts compatible to BBF RFC 53. The MPF and MSF is the abbreviation of meta-prefix and meta-suffix seperately. Because pduA is said to be the shell protein of pdu BMC, this part can be used to direct pduA-RFP chimera into BMC lumun. false false _498_ 0 3984 9 It's complicated true .. false Yang Zhang annotation2112719 1 start codon range2112719 1 1 3 annotation2112720 1 pduA coding sequence range2112720 1 1 282 BBa_K371054 1 BBa_K371054 MPF(meta-prefix)+[GFP+10*GS+A] fusion protein+MSF(meta-suffix)) 2010-10-27T11:00:00Z 2015-05-08T01:12:16Z aa aa false true _498_ 0 3984 9 It's complicated true aa false Yang Zhang component2112748 1 BBa_K371024 component2112756 1 BBa_K371050 component2112743 1 BBa_K371049 component2112746 1 BBa_K371021 component2112751 1 BBa_K371037 annotation2112751 1 BBa_K371037 range2112751 1 758 1039 annotation2112748 1 BBa_K371024 range2112748 1 728 757 annotation2112756 1 BBa_K371050 range2112756 1 1040 1049 annotation2112743 1 BBa_K371049 range2112743 1 1 10 annotation2112746 1 BBa_K371021 range2112746 1 11 727 BBa_K371021 1 BBa_K371021 GFP[intermediate] 2010-10-26T11:00:00Z 2015-05-08T01:12:16Z the origin sequence of this part comes from BioBrick BBa_E1010 this part is a intermediate part, to see the detail design consideration please go to BBa_K371021 false false _498_ 0 3984 9 Not in stock false this part is a intermediate part used to generate part BBa_K371021 false Yang Zhang annotation2109385 1 start codon range2109385 1 1 3 annotation2109384 1 GFP range2109384 1 1 717 BBa_K371021_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaggt BBa_K371050_sequence 1 tgaagagatc BBa_K371024_sequence 1 ggtagcggcagcggtagcggtagcggcagc BBa_K371054_sequence 1 gagctcttcaatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaggtggtagcggcagcggtagcggtagcggcagcatgcaacaagaagcgttaggaatggtagaaaccaaaggcttgacagcagccatagaggccgcagatgcaatggtgaagtcagccaatgtaatgctggtcggctacgaaaaaattggttcggggctggtaacagttattgtccgcggcgatgtcggcgcagtcaaagcagcaacagatgccggtgccgccgcagcccgtaatgtgggagaagtgaaagccgtacacgttatcccacgcccgcataccgatgtagaaaaaatcttaccgaagggaattagcggttgaagagatc BBa_K371049_sequence 1 gagctcttca BBa_K371037_sequence 1 atgcaacaagaagcgttaggaatggtagaaaccaaaggcttgacagcagccatagaggccgcagatgcaatggtgaagtcagccaatgtaatgctggtcggctacgaaaaaattggttcggggctggtaacagttattgtccgcggcgatgtcggcgcagtcaaagcagcaacagatgccggtgccgccgcagcccgtaatgtgggagaagtgaaagccgtacacgttatcccacgcccgcataccgatgtagaaaaaatcttaccgaagggaattagcggt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z