BBa_K371061
type: ComponentDefinition
persistent identity: BBa K371061
display id: BBa_K371061
version: 1
was generated by: iGEM to SBOL conversion
description: MPF-GGTTAATAA-B0034-ATG-MSF MetaPart
created: 2010-11-05T12:00:00Z
modified: 2015-05-08T01:12:16Z
mutable provenance: synthesis
owned by: james
owned by: myers
mutable description: MPF-GGTTAATAA-B0034-ATG-MSF MetaPart
discontinued: false
dominant: false
group u list: _498_
m user id: 0
owner id: 3908
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: MPF-GGTTAATAA-B0034-ATG-MSF MetaPart
star: false
creator: Hao Jiang
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
component: BBa_K371058
component: BBa_K371059
sequence annotation: BBa_K371059
sequence annotation: BBa_K371058
sequence: https://synbiohub.org/public/igem/BBa_K371061_sequence/1

BBa_K371058
type: Component
persistent identity: component2113912
display id: component2113912
version: 1
top level: BBa_K371061
definition: BBa_K371058
access: public

BBa_K371059
type: Component
persistent identity: component2113902
display id: component2113902
version: 1
top level: BBa_K371061
definition: BBa_K371059
access: public

BBa_K371059
type: SequenceAnnotation
persistent identity: annotation2113902
display id: annotation2113902
version: 1
top level: BBa_K371061
location: https://synbiohub.org/public/igem/BBa_K371061/annotation2113902/range2113902/1
component: BBa_K371059

https://synbiohub.org/public/igem/BBa_K371061/annotation2113902/range2113902/1
type: Range
persistent identity: range2113902
display id: range2113902
version: 1
top level: BBa_K371061
direction: direction/reverse
start: 1
end: 19
orientation: inline

BBa_K371058
type: SequenceAnnotation
persistent identity: annotation2113912
display id: annotation2113912
version: 1
top level: BBa_K371061
location: https://synbiohub.org/public/igem/BBa_K371061/annotation2113912/range2113912/1
component: BBa_K371058

https://synbiohub.org/public/igem/BBa_K371061/annotation2113912/range2113912/1
type: Range
persistent identity: range2113912
display id: range2113912
version: 1
top level: BBa_K371061
direction: direction/reverse
start: 28
end: 58
orientation: inline

BBa_K371058
type: ComponentDefinition
persistent identity: BBa K371058
display id: BBa_K371058
version: 1
was generated by: iGEM to SBOL conversion
description: B0034-ATG-MSF HeadPart
created: 2010-11-05T12:00:00Z
modified: 2015-05-08T01:12:16Z
mutable provenance: synthesis
owned by: myers
owned by: james
mutable description: B0034-ATG-MSF HeadPart
discontinued: false
dominant: false
group u list: _498_
m user id: 0
owner id: 3908
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: B0034-ATG-MSF HeadPart
star: false
creator: Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K371057
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K371057
sequence: https://synbiohub.org/public/igem/BBa_K371058_sequence/1

BBa_K371057
type: Component
persistent identity: component2113877
display id: component2113877
version: 1
top level: BBa_K371058
definition: BBa_K371057
access: public

BBa_B0034
type: Component
persistent identity: component2113870
display id: component2113870
version: 1
top level: BBa_K371058
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2113870
display id: annotation2113870
version: 1
top level: BBa_K371058
location: https://synbiohub.org/public/igem/BBa_K371058/annotation2113870/range2113870/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K371058/annotation2113870/range2113870/1
type: Range
persistent identity: range2113870
display id: range2113870
version: 1
top level: BBa_K371058
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K371057
type: SequenceAnnotation
persistent identity: annotation2113877
display id: annotation2113877
version: 1
top level: BBa_K371058
location: https://synbiohub.org/public/igem/BBa_K371058/annotation2113877/range2113877/1
component: BBa_K371057

https://synbiohub.org/public/igem/BBa_K371058/annotation2113877/range2113877/1
type: Range
persistent identity: range2113877
display id: range2113877
version: 1
top level: BBa_K371058
direction: direction/reverse
start: 19
end: 31
orientation: inline

BBa_K371059
type: ComponentDefinition
persistent identity: BBa K371059
display id: BBa_K371059
version: 1
was generated by: iGEM to SBOL conversion
description: MPF-GGTTAATAA TailPart
created: 2010-11-05T12:00:00Z
modified: 2015-05-08T01:12:16Z
mutable provenance: synthesis
owned by: james
owned by: myers
mutable description: MPF-TAATAA TailPart
discontinued: false
dominant: false
experience: experience/None
group u list: _498_
m user id: 0
owner id: 3908
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: MPF-TAATAA TailPart
star: false
creator: Hao Jiang
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
component: BBa_K371025
component: BBa_K371060
component: BBa_K371049
sequence annotation: BBa_K371049
sequence annotation: BBa_K371060
sequence annotation: BBa_K371025
sequence: https://synbiohub.org/public/igem/BBa_K371059_sequence/1

BBa_K371025
type: Component
persistent identity: component2113893
display id: component2113893
version: 1
top level: BBa_K371059
definition: BBa_K371025
access: public

BBa_K371060
type: Component
persistent identity: component2113891
display id: component2113891
version: 1
top level: BBa_K371059
definition: BBa_K371060
access: public

BBa_K371049
type: Component
persistent identity: component2113890
display id: component2113890
version: 1
top level: BBa_K371059
definition: BBa_K371049
access: public

BBa_K371049
type: SequenceAnnotation
persistent identity: annotation2113890
display id: annotation2113890
version: 1
top level: BBa_K371059
location: https://synbiohub.org/public/igem/BBa_K371059/annotation2113890/range2113890/1
component: BBa_K371049

https://synbiohub.org/public/igem/BBa_K371059/annotation2113890/range2113890/1
type: Range
persistent identity: range2113890
display id: range2113890
version: 1
top level: BBa_K371059
direction: direction/reverse
start: 1
end: 10
orientation: inline

BBa_K371060
type: SequenceAnnotation
persistent identity: annotation2113891
display id: annotation2113891
version: 1
top level: BBa_K371059
location: https://synbiohub.org/public/igem/BBa_K371059/annotation2113891/range2113891/1
component: BBa_K371060

https://synbiohub.org/public/igem/BBa_K371059/annotation2113891/range2113891/1
type: Range
persistent identity: range2113891
display id: range2113891
version: 1
top level: BBa_K371059
direction: direction/reverse
start: 11
end: 13
orientation: inline

BBa_K371025
type: SequenceAnnotation
persistent identity: annotation2113893
display id: annotation2113893
version: 1
top level: BBa_K371059
location: https://synbiohub.org/public/igem/BBa_K371059/annotation2113893/range2113893/1
component: BBa_K371025

https://synbiohub.org/public/igem/BBa_K371059/annotation2113893/range2113893/1
type: Range
persistent identity: range2113893
display id: range2113893
version: 1
top level: BBa_K371059
direction: direction/reverse
start: 14
end: 19
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K371049
type: ComponentDefinition
persistent identity: BBa K371049
display id: BBa_K371049
version: 1
was generated by: iGEM to SBOL conversion
description: MPF(meta-prefix)
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:16Z
mutable provenance: direct synthesis
owned by: james
owned by: myers
mutable description: This part is a extention of BioBrick prefix. It is used in noval BioBrick assemble standard BBF RFC 53.
discontinued: false
dominant: false
experience: experience/Works
group u list: _498_
m user id: 0
owner id: 3984
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: BBF RFC53
star: false
creator: Yang Zhang
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: SacI
sequence annotation: meta-prefix
sequence annotation: SapI
sequence annotation: EarI
sequence: https://synbiohub.org/public/igem/BBa_K371049_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation2105194
display id: annotation2105194
version: 1
top level: BBa_K371049
location: https://synbiohub.org/public/igem/BBa_K371049/annotation2105194/range2105194/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K371049/annotation2105194/range2105194/1
type: Range
persistent identity: range2105194
display id: range2105194
version: 1
top level: BBa_K371049
direction: direction/forward
start: 1
end: 6
orientation: inline

meta-prefix
type: SequenceAnnotation
persistent identity: annotation2105193
display id: annotation2105193
version: 1
top level: BBa_K371049
location: https://synbiohub.org/public/igem/BBa_K371049/annotation2105193/range2105193/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K371049/annotation2105193/range2105193/1
type: Range
persistent identity: range2105193
display id: range2105193
version: 1
top level: BBa_K371049
direction: direction/forward
start: 1
end: 10
orientation: inline

SapI
type: SequenceAnnotation
persistent identity: annotation2105192
display id: annotation2105192
version: 1
top level: BBa_K371049
location: https://synbiohub.org/public/igem/BBa_K371049/annotation2105192/range2105192/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K371049/annotation2105192/range2105192/1
type: Range
persistent identity: range2105192
display id: range2105192
version: 1
top level: BBa_K371049
direction: direction/forward
start: 3
end: 9
orientation: inline

EarI
type: SequenceAnnotation
persistent identity: annotation2105195
display id: annotation2105195
version: 1
top level: BBa_K371049
location: https://synbiohub.org/public/igem/BBa_K371049/annotation2105195/range2105195/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K371049/annotation2105195/range2105195/1
type: Range
persistent identity: range2105195
display id: range2105195
version: 1
top level: BBa_K371049
direction: direction/forward
start: 4
end: 9
orientation: inline

BBa_K371025
type: ComponentDefinition
persistent identity: BBa K371025
display id: BBa_K371025
version: 1
was generated by: iGEM to SBOL conversion
description: TAATAA[intermediate]
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:16Z
mutable provenance: ~~~
owned by: james
owned by: myers
mutable description: This part consists of two stop codon taa. It is a intermidate part use to construct a complete fusion protein. For more disign consideration, please go to BBa_K371030 or BBa_K371031
discontinued: false
dominant: false
group u list: _498_
m user id: 0
owner id: 3984
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: ~~~
star: false
creator: Yang Zhang
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_K371025_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation2109483
display id: annotation2109483
version: 1
top level: BBa_K371025
location: https://synbiohub.org/public/igem/BBa_K371025/annotation2109483/range2109483/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K371025/annotation2109483/range2109483/1
type: Range
persistent identity: range2109483
display id: range2109483
version: 1
top level: BBa_K371025
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K371057
type: ComponentDefinition
persistent identity: BBa K371057
display id: BBa_K371057
version: 1
was generated by: iGEM to SBOL conversion
description: ATG-MSF HeadPart
created: 2010-11-05T12:00:00Z
modified: 2015-05-08T01:12:16Z
mutable provenance: synthesis
owned by: james
owned by: myers
mutable description: ATG-MSF HeadPart
discontinued: false
dominant: false
group u list: _498_
m user id: 0
owner id: 3908
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: ATG-MSF HeadPart
star: false
creator: Hao Jiang
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
component: BBa_K371050
component: BBa_K371056
sequence annotation: BBa_K371056
sequence annotation: BBa_K371050
sequence: https://synbiohub.org/public/igem/BBa_K371057_sequence/1

BBa_K371050
type: Component
persistent identity: component2113868
display id: component2113868
version: 1
top level: BBa_K371057
definition: BBa_K371050
access: public

BBa_K371056
type: Component
persistent identity: component2113863
display id: component2113863
version: 1
top level: BBa_K371057
definition: BBa_K371056
access: public

BBa_K371056
type: SequenceAnnotation
persistent identity: annotation2113863
display id: annotation2113863
version: 1
top level: BBa_K371057
location: https://synbiohub.org/public/igem/BBa_K371057/annotation2113863/range2113863/1
component: BBa_K371056

https://synbiohub.org/public/igem/BBa_K371057/annotation2113863/range2113863/1
type: Range
persistent identity: range2113863
display id: range2113863
version: 1
top level: BBa_K371057
direction: direction/reverse
start: 1
end: 3
orientation: inline

BBa_K371050
type: SequenceAnnotation
persistent identity: annotation2113868
display id: annotation2113868
version: 1
top level: BBa_K371057
location: https://synbiohub.org/public/igem/BBa_K371057/annotation2113868/range2113868/1
component: BBa_K371050

https://synbiohub.org/public/igem/BBa_K371057/annotation2113868/range2113868/1
type: Range
persistent identity: range2113868
display id: range2113868
version: 1
top level: BBa_K371057
direction: direction/reverse
start: 4
end: 13
orientation: inline

BBa_K371060
type: ComponentDefinition
persistent identity: BBa K371060
display id: BBa_K371060
version: 1
was generated by: iGEM to SBOL conversion
description: GGT
created: 2010-11-05T12:00:00Z
modified: 2015-05-08T01:12:16Z
mutable provenance: GGT
owned by: james
owned by: myers
mutable description: GGT, codon for glycine.
discontinued: false
dominant: false
group u list: _498_
m user id: 0
owner id: 3908
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: GGT
star: false
creator: Hao Jiang
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K371060_sequence/1

BBa_K371050
type: ComponentDefinition
persistent identity: BBa K371050
display id: BBa_K371050
version: 1
was generated by: iGEM to SBOL conversion
description: MSF(meta-suffix)
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:16Z
mutable provenance: direct synthesis
owned by: james
owned by: myers
mutable description: This part is a extention of BioBrick prefix. It is used in noval BioBrick assemble standard BBF RFC 53.
discontinued: false
dominant: false
experience: experience/Works
group u list: _498_
m user id: 0
owner id: 3984
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: BBF RFC53
star: false
creator: Yang Zhang
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: stop codon
sequence annotation: MSF
sequence annotation: EarI
sequence annotation: BglII
sequence: https://synbiohub.org/public/igem/BBa_K371050_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation2110071
display id: annotation2110071
version: 1
top level: BBa_K371050
location: https://synbiohub.org/public/igem/BBa_K371050/annotation2110071/range2110071/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K371050/annotation2110071/range2110071/1
type: Range
persistent identity: range2110071
display id: range2110071
version: 1
top level: BBa_K371050
direction: direction/forward
start: 1
end: 3
orientation: inline

MSF
type: SequenceAnnotation
persistent identity: annotation2105198
display id: annotation2105198
version: 1
top level: BBa_K371050
location: https://synbiohub.org/public/igem/BBa_K371050/annotation2105198/range2105198/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K371050/annotation2105198/range2105198/1
type: Range
persistent identity: range2105198
display id: range2105198
version: 1
top level: BBa_K371050
direction: direction/forward
start: 1
end: 10
orientation: inline

EarI
type: SequenceAnnotation
persistent identity: annotation2105197
display id: annotation2105197
version: 1
top level: BBa_K371050
location: https://synbiohub.org/public/igem/BBa_K371050/annotation2105197/range2105197/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K371050/annotation2105197/range2105197/1
type: Range
persistent identity: range2105197
display id: range2105197
version: 1
top level: BBa_K371050
direction: direction/forward
start: 2
end: 7
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation2105196
display id: annotation2105196
version: 1
top level: BBa_K371050
location: https://synbiohub.org/public/igem/BBa_K371050/annotation2105196/range2105196/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K371050/annotation2105196/range2105196/1
type: Range
persistent identity: range2105196
display id: range2105196
version: 1
top level: BBa_K371050
direction: direction/forward
start: 6
end: 10
orientation: inline

BBa_K371056
type: ComponentDefinition
persistent identity: BBa K371056
display id: BBa_K371056
version: 1
was generated by: iGEM to SBOL conversion
description: ATG
created: 2010-11-05T12:00:00Z
modified: 2015-05-08T01:12:16Z
mutable provenance: ATG
owned by: james
owned by: myers
mutable description: ATG
discontinued: false
dominant: false
experience: experience/None
group u list: _498_
m user id: 0
owner id: 3908
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: ATG
star: false
creator: Hao Jiang
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K371056_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2113878
display id: annotation2113878
version: 1
top level: BBa_K371056
location: https://synbiohub.org/public/igem/BBa_K371056/annotation2113878/range2113878/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K371056/annotation2113878/range2113878/1
type: Range
persistent identity: range2113878
display id: range2113878
version: 1
top level: BBa_K371056
direction: direction/forward
start: 1
end: 3
orientation: inline

https://synbiohub.org/public/igem/BBa_K371061_sequence/1
type: Sequence
persistent identity: BBa K371061 sequence
display id: BBa_K371061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagctcttcaggttaataatactagagaaagaggagaaatactagatgtgaagagatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371058_sequence/1
type: Sequence
persistent identity: BBa K371058 sequence
display id: BBa_K371058_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtgaagagatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371059_sequence/1
type: Sequence
persistent identity: BBa K371059 sequence
display id: BBa_K371059_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagctcttcaggttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371049_sequence/1
type: Sequence
persistent identity: BBa K371049 sequence
display id: BBa_K371049_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagctcttca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371025_sequence/1
type: Sequence
persistent identity: BBa K371025 sequence
display id: BBa_K371025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371057_sequence/1
type: Sequence
persistent identity: BBa K371057 sequence
display id: BBa_K371057_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtgaagagatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371060_sequence/1
type: Sequence
persistent identity: BBa K371060 sequence
display id: BBa_K371060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371050_sequence/1
type: Sequence
persistent identity: BBa K371050 sequence
display id: BBa_K371050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgaagagatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K371056_sequence/1
type: Sequence
persistent identity: BBa K371056 sequence
display id: BBa_K371056_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z