LOCUS       BBa_K373002             2262 bp    DNA     linear       26-Dec-2024
DEFINITION  {Pbad.rbs.prepro.StrepTag}[PYY3-36]{INP repeats}{VtaA11 
            AtD}(dblterm)
ACCESSION   BBa_K373002
VERSION     BBa_K373002.1  
FEATURES             Location/Qualifiers
     CDS             50..805
                     /label=AraC
     promoter        956..1193
                     /label=Pbad
     CDS             1290..1358
                     /label=PrePro Sequence
     CDS             1374..1412
                     /label=Streptavidin Binding Peptide
     RBS             1274..1289
                     /label=rbs
     misc_feature    1..1452
                     /label=BBa_M10093
     misc_feature    1459..1461
                     /label=Start
     misc_feature    1564..1569
                     /label=Stop
     CDS             1462..1563
                     /label=PYY3-36 coding region
     CDS             1459..1569
                     /label=BBa_K373000
     CDS             1578..1874
                     /label=BBa_K197026
     CDS             1883..2125
                     /label=BBa_K197004
     stem_loop       2145..2188
                     /label=stem_loop
     misc_feature    2134..2213
                     /label=BBa_B0010
     terminator      2134..2213
                     /label=BBa_B0010
     stem_loop       2229..2248
                     /label=T7 TE
     misc_feature    2255..2255
                     /label=stop
     polyA_site      2249..2262
                     /label=polya
     misc_feature    2222..2262
                     /label=BBa_B0012
     terminator      2222..2262
                     /label=BBa_B0012
ORIGIN
        1 ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg
       61 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
      121 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
      181 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
      241 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac
      301 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
      361 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
      421 ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc
      481 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc
      541 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta
      601 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga
      661 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc
      721 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
      781 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
      841 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg
      901 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat
      961 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc
     1021 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca
     1081 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac
     1141 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcttacctga
     1201 cgctttttat cgcaactctc tactgtttct ccatacccgg atctttgggc tagcgcattc
     1261 gagctcggta cctttgaggt ggttatgaaa aaaattgcat gtctttcagc actggccgca
     1321 gttctggctt tcaccgcagg tacttccgta gctggccagt ctggccagtg ggtgtgccac
     1381 ccgatgtggg aggtgatgtg cctgagggga gggtctgggt ggaggggcat ggagggaggg
     1441 cagtctgggc agtactagat gtagtttggg ctccgagggc cgcttctgcg gagcggcctc
     1501 ctcgacttgg cgatgatgcg gagggacgcg gtgatggagt tggaccagtg ggccgtcgcc
     1561 atataataat actagagaac cacagtgatc taattgccgg ttatggaagt acaggcaccg
     1621 ccggctccga cagctggctg gtcgctggct atggaagcac ccagaccgcc ggtggggaca
     1681 gcgcgctgac agcgggttac ggcagcaccc agaccgcccg cgaaggcagc aacctgacgg
     1741 cagggtacgg cagcaccggc acggcaggct cggacagttc gctgatcgcc ggttacggca
     1801 gtactcagac ttcgggcggg gacagctcac tcactgcggg ctacggcagc actgcaacgg
     1861 ccgggcagga cagttactag aggatctaac aacaaagctc tgcgtgctgg tatcgctggt
     1921 tctaacgctg ctgctggtct gccgcaggtt tatattccgg gtaaatctat ggttgctgtt
     1981 tctgctggca ctttcaaagg tcagtctgct ctggctgttg gttactctcg tgcttctgac
     2041 aacggtaaac tgatcctgaa actccaaggt aacgctaaca cctctggtga aatgggtggt
     2101 tctgttggtg ttggttacca gtgggtacta gagccaggca tcaaataaaa cgaaaggctc
     2161 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     2221 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//