BBa_K374009
1
BBa_K374009
Gifsy2 promoters and Gifsy2 repressor gtgR
2010-10-19T11:00:00Z
2015-05-08T01:12:16Z
Genomic sequence from the Salmonella phage Gifsy2
Gifsy2 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy2 promoters and the Gifsy2 repressor. The promoters are divergent; consisting of pR[Gifsy2] and pRM[Gifsy2]. Downstream of the pRM[Gifsy2] promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy2] and a repressor of the pR[Gifsy2]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in E. Coli.
false
false
_520_
0
7351
9
It's complicated
true
The part contains both promoters and repressor. We chose not to separate the repressor gene from the promoter because we wanted to keep the natural RBS.
false
Lisa Blanc Iversen, Maya Friis Kjaergaard, Annemi Jollmann, Anja Sander and Grzegorz Słodkowicz
annotation2090403
1
pR
range2090403
1
491
496
annotation2090401
1
gtgR
range2090401
1
1
411
annotation2090402
1
pRM
range2090402
1
418
423
BBa_K374009_sequence
1
ctattttttgaggtcgttaattagatcaaaaacgttgttcttcagcagatccatctcttggaggactgcttttgtatggaggataacacgcagtttctctgcttccgggagttgattgaaaagagaaagcaatgtttcttctttttcatcaaggactctgggtttaggttcagccctttcgccttcactttcatcccctggctccatgaagaaccaatattcaggccttccggtcaccgccgataaccttttcaagcgctctccgctggctacagacgtgccagaggcccactttctcacagatgtgtgagaaagcattacgcgccgtgagaggtcagccatagaccagccgttctcatccagaacttgctggatacgcttggcgaaaatgggatgaagatttttgttcatattcacattttacaaccttcagtttctttattcattggaactattagattgatttttgttggaacctaaagttttacgtgataat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z