##gff-version 3
##sequence-region BBa_K376016 1 1708
BBa_K376016	.	engineered_region	1	858	.	+	0	ID=BBa_K376009;Name=BBa_K376009
BBa_K376016	.	promoter	1	126	.	+	0	ID=BBa_K091146;Name=BBa_K091146;Parent=BBa_K376009
BBa_K376016	.	start_codon	125	125	.	+	0	ID=annotation1967358;Name=Transcriptional Start;Parent=BBa_K091146
BBa_K376016	.	sequence_feature	113	118	.	+	0	ID=annotation1967356;Name=-10;Parent=BBa_K091146
BBa_K376016	.	non_covalent_binding_site	75	93	.	+	0	ID=annotation1967354;Name=LasR;Parent=BBa_K091146
BBa_K376016	.	non_covalent_binding_site	94	113	.	+	0	ID=annotation1967355;Name=LuxR;Parent=BBa_K091146
BBa_K376016	.	sequence_feature	86	91	.	+	0	ID=annotation1967357;Name=-35;Parent=BBa_K091146
BBa_K376016	.	engineered_region	135	858	.	+	0	ID=BBa_I13502;Name=BBa_I13502;Parent=BBa_K376009
BBa_K376016	.	ribosome_entry_site	135	146	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_K376016	.	conserved_region	139	142	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K376016	.	CDS	153	833	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_K376016	.	engineered_tag	834	858	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K376016	.	CDS	153	827	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K376016	.	engineered_region	867	1708	.	+	0	ID=BBa_K376012;Name=BBa_K376012
BBa_K376016	.	promoter	867	901	.	+	0	ID=BBa_J23108;Name=BBa_J23108;Parent=BBa_K376012
BBa_K376016	.	engineered_region	910	1708	.	+	0	ID=BBa_K376010;Name=BBa_K376010;Parent=BBa_K376012
>BBa_K376016
tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatcta
tctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatggct
tcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaa
tcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgcc
gttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggac
tacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgtta
cccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtcc
ggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaa
atcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccgg
ttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaaca
gtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagctg
acagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgac
gacacatacagaataattaataaaattaaagcttgtagaagcaatattgatattaatcaatgcttatctgatatgacta
aaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctaga
taattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtaggttattctaac
tccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaatctccaaatgtaattaaagaagc
gaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgca
cattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctag
ttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagc
gtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccattta
accaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgatt
gcccatactttaaaaattaataacactgatagtgctagtgtagatcact