LOCUS       BBa_K376025             2509 bp    DNA     linear       16-Dec-2024
DEFINITION  LuxI+Tet Inverter+ Lambda Repressor
ACCESSION   BBa_K376025
VERSION     BBa_K376025.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    598..629
                     /label=LVA
     CDS             19..597
                     /label=luxI
     misc_feature    19..636
                     /label=BBa_C0061
     misc_feature    637..661
                     /label=Help:Barcodes
     CDS             19..636
                     /label=BBa_C0061
     misc_feature    1..661
                     /label=BBa_C0261
     misc_feature    674..677
                     /label=conserved
     RBS             670..681
                     /label=BBa_B0034
     CDS             691..1307
                     /label=tetR
     misc_feature    1308..1341
                     /label=SsrA
     misc_feature    1348..1372
                     /label=Help:Barcodes
     CDS             688..1347
                     /label=BBa_C0040
     stem_loop       1392..1435
                     /label=stem_loop
     misc_feature    1381..1460
                     /label=BBa_B0010
     terminator      1381..1460
                     /label=BBa_B0010
     stem_loop       1476..1495
                     /label=T7 TE
     misc_feature    1502..1502
                     /label=stop
     polyA_site      1496..1509
                     /label=polya
     misc_feature    1469..1509
                     /label=BBa_B0012
     terminator      1469..1509
                     /label=BBa_B0012
     misc_feature    1518..1536
                     /label=TetR 1
     misc_feature    1518..1571
                     /label=BBa_R0040
     promoter        1537..1542
                     /label=-35
     misc_feature    1543..1561
                     /label=TetR 2
     promoter        1560..1565
                     /label=-10
     promoter        1518..1571
                     /label=BBa_R0040
     misc_feature    670..1571
                     /label=BBa_Q04400
     misc_feature    1584..1587
                     /label=conserved
     RBS             1580..1591
                     /label=BBa_B0034
     CDS             1601..2308
                     /label=cI lambda
     misc_feature    2309..2341
                     /label=LVA
     misc_feature    2348..2372
                     /label=Help:Barcodes
     CDS             1598..2347
                     /label=BBa_C0051
     stem_loop       2392..2435
                     /label=stem_loop
     misc_feature    2381..2460
                     /label=BBa_B0010
     terminator      2381..2460
                     /label=BBa_B0010
     stem_loop       2476..2495
                     /label=T7 TE
     misc_feature    2502..2502
                     /label=stop
     polyA_site      2496..2509
                     /label=polya
     misc_feature    2469..2509
                     /label=BBa_B0012
     terminator      2469..2509
                     /label=BBa_B0012
     misc_feature    1580..2509
                     /label=BBa_P0451
     misc_feature    670..2509
                     /label=BBa_K376019
ORIGIN
        1 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca
       61 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag
      121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa
      181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca
      241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc
      301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata
      361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt
      421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag
      481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact
      541 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct
      601 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct
      661 ctactagaga aagaggagaa atactagatg tccagattag ataaaagtaa agtgattaac
      721 agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc
      781 cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg
      841 ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa
      901 ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta
      961 agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa
     1021 actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta
     1081 tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat
     1141 caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga
     1201 caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa
     1261 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac
     1321 gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag
     1381 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1441 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1501 gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc agtgatagag
     1561 atactgagca ctactagaga aagaggagaa atactagatg agcacaaaaa agaaaccatt
     1621 aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa
     1681 tgaacttggc ttatcccagg aatctgtcgc agacaagatg gggatggggc agtcaggcgt
     1741 tggtgcttta tttaatggca tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa
     1801 aattctcaaa gttagcgttg aagaatttag cccttcaatc gccagagaaa tctacgagat
     1861 gtatgaagcg gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc
     1921 tcatgttcag gcagggatgt tctcacctga gcttagaacc tttaccaaag gtgatgcgga
     1981 gagatgggta agcacaacca aaaaagccag tgattctgca ttctggcttg aggttgaagg
     2041 taattccatg accgcaccaa caggctccaa gccaagcttt cctgacggaa tgttaattct
     2101 cgttgaccct gagcaggctg ttgagccagg tgatttctgc atagccagac ttgggggtga
     2161 tgagtttacc ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa
     2221 cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc
     2281 tagtcagtgg cctgaagaga cgtttggcgc tgcaaacgac gaaaactacg ctttagtagc
     2341 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
     2401 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2461 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
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