##gff-version 3
##sequence-region BBa_K376029 1 3359
BBa_K376029	.	engineered_region	1	2509	.	+	0	ID=BBa_K376025;Name=BBa_K376025
BBa_K376029	.	engineered_region	670	2509	.	+	0	ID=BBa_K376019;Name=BBa_K376019;Parent=BBa_K376025
BBa_K376029	.	engineered_region	670	1571	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400;Parent=BBa_K376019
BBa_K376029	.	terminator	1469	1509	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_K376029	.	engineered_region	1469	1509	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K376029	.	stem_loop	1476	1495	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K376029	.	polyA_site	1496	1509	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K376029	.	stop_codon	1502	1502	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K376029	.	terminator	1381	1460	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_K376029	.	engineered_region	1381	1460	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K376029	.	stem_loop	1392	1435	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K376029	.	promoter	1518	1571	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_K376029	.	promoter	1560	1565	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K376029	.	promoter	1537	1542	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K376029	.	engineered_region	1518	1571	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K376029	.	non_covalent_binding_site	1543	1561	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K376029	.	non_covalent_binding_site	1518	1536	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K376029	.	CDS	688	1347	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_K376029	.	CDS	691	1307	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K376029	.	engineered_tag	1348	1372	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K376029	.	tag	1308	1341	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K376029	.	ribosome_entry_site	670	681	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_K376029	.	conserved_region	674	677	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K376029	.	engineered_region	1580	2509	.	+	0	ID=BBa_P0451;Name=BBa_P0451;Parent=BBa_K376019
BBa_K376029	.	ribosome_entry_site	1580	1591	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0451
BBa_K376029	.	conserved_region	1584	1587	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K376029	.	terminator	2469	2509	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0451
BBa_K376029	.	engineered_region	2469	2509	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K376029	.	stem_loop	2476	2495	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K376029	.	polyA_site	2496	2509	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K376029	.	stop_codon	2502	2502	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K376029	.	terminator	2381	2460	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0451
BBa_K376029	.	engineered_region	2381	2460	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K376029	.	stem_loop	2392	2435	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K376029	.	CDS	1598	2347	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0451
BBa_K376029	.	tag	2309	2341	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K376029	.	engineered_tag	2348	2372	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K376029	.	CDS	1601	2308	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K376029	.	engineered_region	1	661	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K376025
BBa_K376029	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K376029	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K376029	.	CDS	19	636	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K376029	.	CDS	19	597	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K376029	.	tag	598	629	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K376029	.	engineered_tag	637	661	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K376029	.	engineered_region	19	636	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K376029	.	engineered_region	2518	3359	.	+	0	ID=BBa_K376013;Name=BBa_K376013
BBa_K376029	.	engineered_region	2561	3359	.	+	0	ID=BBa_K376010;Name=BBa_K376010;Parent=BBa_K376013
BBa_K376029	.	promoter	2518	2552	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K376013
>BBa_K376029
aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggta
ttctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatga
gtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacct
acaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatag
tcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatt
tgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgattt
ttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtat
tgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgtccagattagataaaagtaaa
gtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctag
gtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttaga
taggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttaga
tgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcg
aaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattt
tactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatg
ccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattga
tcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagctta
ataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagaga
aagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagc
aatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggc
gttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcg
ttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaag
tgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgat
gcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccg
caccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtga
tttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaa
ccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggc
ctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatc
gctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctag
ctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacata
cagaataattaataaaattaaagcttgtagaagcaatattgatattaatcaatgcttatctgatatgactaaaatggta
cattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattacc
ctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtaggttattctaactccaatca
ttcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaatctccaaatgtaattaaagaagcgaaaacat
caggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcaga
aaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataat
tatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcat
gcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgc
gcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatac
tttaaaaattaataacactgatagtgctagtgtagatcact