##gff-version 3
##sequence-region BBa_K376031 1 3906
BBa_K376031	.	engineered_region	1	539	.	+	0	ID=BBa_K376005;Name=BBa_K376005
BBa_K376031	.	promoter	1	83	.	+	0	ID=BBa_K376003;Name=BBa_K376003;Parent=BBa_K376005
BBa_K376031	.	engineered_region	92	539	.	+	0	ID=BBa_K376004;Name=BBa_K376004;Parent=BBa_K376005
BBa_K376031	.	CDS	123	539	.	+	0	ID=annotation2107921;Name=Anaerobic Flourescent Protein;Parent=BBa_K376004
BBa_K376031	.	ribosome_entry_site	92	117	.	+	0	ID=annotation2107920;Name=Super RBS;Parent=BBa_K376004
BBa_K376031	.	engineered_region	548	3906	.	+	0	ID=BBa_K376028;Name=BBa_K376028
BBa_K376031	.	engineered_region	548	3056	.	+	0	ID=BBa_K376025;Name=BBa_K376025;Parent=BBa_K376028
BBa_K376031	.	engineered_region	1217	3056	.	+	0	ID=BBa_K376019;Name=BBa_K376019;Parent=BBa_K376025
BBa_K376031	.	engineered_region	2127	3056	.	+	0	ID=BBa_P0451;Name=BBa_P0451;Parent=BBa_K376019
BBa_K376031	.	terminator	2928	3007	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0451
BBa_K376031	.	stem_loop	2939	2982	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K376031	.	engineered_region	2928	3007	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K376031	.	terminator	3016	3056	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0451
BBa_K376031	.	engineered_region	3016	3056	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K376031	.	stop_codon	3049	3049	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K376031	.	stem_loop	3023	3042	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K376031	.	polyA_site	3043	3056	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K376031	.	ribosome_entry_site	2127	2138	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0451
BBa_K376031	.	conserved_region	2131	2134	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K376031	.	CDS	2145	2894	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0451
BBa_K376031	.	tag	2856	2888	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K376031	.	CDS	2148	2855	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K376031	.	engineered_tag	2895	2919	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K376031	.	engineered_region	1217	2118	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400;Parent=BBa_K376019
BBa_K376031	.	ribosome_entry_site	1217	1228	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_K376031	.	conserved_region	1221	1224	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K376031	.	CDS	1235	1894	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_K376031	.	engineered_tag	1895	1919	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K376031	.	tag	1855	1888	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K376031	.	CDS	1238	1854	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K376031	.	terminator	2016	2056	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_K376031	.	engineered_region	2016	2056	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K376031	.	stop_codon	2049	2049	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K376031	.	stem_loop	2023	2042	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K376031	.	polyA_site	2043	2056	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K376031	.	promoter	2065	2118	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_K376031	.	promoter	2084	2089	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K376031	.	non_covalent_binding_site	2090	2108	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K376031	.	engineered_region	2065	2118	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K376031	.	non_covalent_binding_site	2065	2083	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K376031	.	promoter	2107	2112	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K376031	.	terminator	1928	2007	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_K376031	.	stem_loop	1939	1982	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K376031	.	engineered_region	1928	2007	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K376031	.	engineered_region	548	1208	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K376025
BBa_K376031	.	CDS	566	1183	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K376031	.	engineered_region	566	1183	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K376031	.	tag	1145	1176	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K376031	.	engineered_tag	1184	1208	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K376031	.	CDS	566	1144	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K376031	.	ribosome_entry_site	548	559	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K376031	.	conserved_region	552	555	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K376031	.	engineered_region	3065	3906	.	+	0	ID=BBa_K376012;Name=BBa_K376012;Parent=BBa_K376028
BBa_K376031	.	engineered_region	3108	3906	.	+	0	ID=BBa_K376010;Name=BBa_K376010;Parent=BBa_K376012
BBa_K376031	.	promoter	3065	3099	.	+	0	ID=BBa_J23108;Name=BBa_J23108;Parent=BBa_K376012
>BBa_K376031
tatattgataaagatcaaccgctaaatatgcgttttgatgaagatcaactgatggctagctcagtcctagggattatgc
tagctactagaggaaattcacacaaatttaaggagagtactagatggcatcgttccaatccttcggcatcccaggtcaa
ttagaagtaatcaaaaaggctctggaccacgtccgcgtgggcgtcgtgatcaccgatccggcgccggaggacaacccga
tcgtttacgtgaatcaaggtttcgttcagatgacgggttacgaaaccgaagagatcctgggcaaaaatgcgcgttttct
ccagggtaagcatacggacccggcggaggttgataacatccgtaccgccttgcagaataaagagccggttaccgtgcaa
attcagaactataagaaagacggtaccatgttttggaacgagctgaacattgacccgatggaaattgaggacaagactt
actttgtcggcattcagaatgatattacgaagcagaaagaatatgagaaactgctggaatgataatactagagaaagag
gagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaa
gtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatga
taactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaaca
ggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaat
taagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagc
tatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaag
cgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtcta
tgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataact
ctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgatt
aacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtag
agcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggca
ccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgct
ttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatc
aattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttacttt
aggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgcca
ttattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatat
gcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataaca
ctgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagagg
agaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaattta
tgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggt
gctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaag
aatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagta
tgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggag
agatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaa
caggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctg
catagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccacta
aacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaag
agacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctgacagctagctcagt
cctaggtataatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaat
aattaataaaattaaagcttgtagaagcaatattgatattaatcaatgcttatctgatatgactaaaatggtacattgt
gaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaa
aatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtaggttattctaactccaatcattcacc
aattaattggaatatatttgaaaacaatgctgtaaataaaaatctccaaatgtaattaaagaagcgaaaacatcaggtc
ttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaaga
caactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcga
aaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaag
gaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaat
gaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaa
aattaataacactgatagtgctagtgtagatcact