##gff-version 3
##sequence-region BBa_K376032 1 3902
BBa_K376032	.	engineered_region	1	535	.	+	0	ID=BBa_K376006;Name=BBa_K376006
BBa_K376032	.	promoter	1	79	.	+	0	ID=BBa_K376002;Name=BBa_K376002;Parent=BBa_K376006
BBa_K376032	.	engineered_region	88	535	.	+	0	ID=BBa_K376004;Name=BBa_K376004;Parent=BBa_K376006
BBa_K376032	.	ribosome_entry_site	88	113	.	+	0	ID=annotation2107920;Name=Super RBS;Parent=BBa_K376004
BBa_K376032	.	CDS	119	535	.	+	0	ID=annotation2107921;Name=Anaerobic Flourescent Protein;Parent=BBa_K376004
BBa_K376032	.	engineered_region	544	3902	.	+	0	ID=BBa_K376028;Name=BBa_K376028
BBa_K376032	.	engineered_region	3061	3902	.	+	0	ID=BBa_K376012;Name=BBa_K376012;Parent=BBa_K376028
BBa_K376032	.	engineered_region	3104	3902	.	+	0	ID=BBa_K376010;Name=BBa_K376010;Parent=BBa_K376012
BBa_K376032	.	promoter	3061	3095	.	+	0	ID=BBa_J23108;Name=BBa_J23108;Parent=BBa_K376012
BBa_K376032	.	engineered_region	544	3052	.	+	0	ID=BBa_K376025;Name=BBa_K376025;Parent=BBa_K376028
BBa_K376032	.	engineered_region	544	1204	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K376025
BBa_K376032	.	ribosome_entry_site	544	555	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K376032	.	conserved_region	548	551	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K376032	.	CDS	562	1179	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K376032	.	CDS	562	1140	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K376032	.	engineered_tag	1180	1204	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K376032	.	engineered_region	562	1179	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K376032	.	tag	1141	1172	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K376032	.	engineered_region	1213	3052	.	+	0	ID=BBa_K376019;Name=BBa_K376019;Parent=BBa_K376025
BBa_K376032	.	engineered_region	2123	3052	.	+	0	ID=BBa_P0451;Name=BBa_P0451;Parent=BBa_K376019
BBa_K376032	.	ribosome_entry_site	2123	2134	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0451
BBa_K376032	.	conserved_region	2127	2130	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K376032	.	terminator	3012	3052	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0451
BBa_K376032	.	stop_codon	3045	3045	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K376032	.	polyA_site	3039	3052	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K376032	.	stem_loop	3019	3038	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K376032	.	engineered_region	3012	3052	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K376032	.	CDS	2141	2890	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0451
BBa_K376032	.	engineered_tag	2891	2915	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K376032	.	CDS	2144	2851	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K376032	.	tag	2852	2884	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K376032	.	terminator	2924	3003	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0451
BBa_K376032	.	engineered_region	2924	3003	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K376032	.	stem_loop	2935	2978	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K376032	.	engineered_region	1213	2114	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400;Parent=BBa_K376019
BBa_K376032	.	terminator	2012	2052	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_K376032	.	stop_codon	2045	2045	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K376032	.	polyA_site	2039	2052	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K376032	.	stem_loop	2019	2038	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K376032	.	engineered_region	2012	2052	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K376032	.	ribosome_entry_site	1213	1224	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_K376032	.	conserved_region	1217	1220	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K376032	.	promoter	2061	2114	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_K376032	.	engineered_region	2061	2114	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K376032	.	promoter	2080	2085	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K376032	.	promoter	2103	2108	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K376032	.	non_covalent_binding_site	2061	2079	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K376032	.	non_covalent_binding_site	2086	2104	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K376032	.	CDS	1231	1890	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_K376032	.	engineered_tag	1891	1915	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K376032	.	CDS	1234	1850	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K376032	.	tag	1851	1884	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K376032	.	terminator	1924	2003	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_K376032	.	engineered_region	1924	2003	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K376032	.	stem_loop	1935	1978	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K376032
gtattatttgatctttatcaaccgctaaaatatgcgttttgatcttcatcaataaaaacagcaacttcaatgtcttaat
tactagaggaaattcacacaaatttaaggagagtactagatggcatcgttccaatccttcggcatcccaggtcaattag
aagtaatcaaaaaggctctggaccacgtccgcgtgggcgtcgtgatcaccgatccggcgccggaggacaacccgatcgt
ttacgtgaatcaaggtttcgttcagatgacgggttacgaaaccgaagagatcctgggcaaaaatgcgcgttttctccag
ggtaagcatacggacccggcggaggttgataacatccgtaccgccttgcagaataaagagccggttaccgtgcaaattc
agaactataagaaagacggtaccatgttttggaacgagctgaacattgacccgatggaaattgaggacaagacttactt
tgtcggcattcagaatgatattacgaagcagaaagaatatgagaaactgctggaatgataatactagagaaagaggaga
aatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtct
tcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataac
tcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtg
attatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaag
tcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctata
tataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgta
ttaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcc
tattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctga
tagtgctagtgtagatctctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaaca
gcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagca
gcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccat
actcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttac
taagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaatt
agcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggt
tgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattat
tacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcgg
attagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactga
tagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
ctagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaa
atactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaa
aaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctt
tatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatt
tagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgag
taccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagat
gggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacagg
ctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcata
gccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacc
cacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagac
gtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctgacagctagctcagtccta
ggtataatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataatt
aataaaattaaagcttgtagaagcaatattgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaat
attatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatg
gaggcaatattatgatgacgctaatttaataaaatatgatcctatagtaggttattctaactccaatcattcaccaatt
aattggaatatatttgaaaacaatgctgtaaataaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttat
cactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaac
tatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaa
taaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaa
aagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaa
ctcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaatt
aataacactgatagtgctagtgtagatcact