LOCUS BBa_K376033 3906 bp DNA linear 16-Jun-2024 DEFINITION Oxygen Sensing AFP Generator+LuxI+Tet Inverter+ Lambda Repressor+LuxR Generator ACCESSION BBa_K376033 VERSION BBa_K376033.1 FEATURES Location/Qualifiers promoter 1..83 /label=BBa_K376003 RBS 92..117 /label=Super RBS CDS 123..539 /label=Anaerobic Flourescent Protein misc_feature 92..539 /label=BBa_K376004 misc_feature 1..539 /label=BBa_K376005 misc_feature 552..555 /label=conserved RBS 548..559 /label=BBa_B0034 misc_feature 1145..1176 /label=LVA CDS 566..1144 /label=luxI misc_feature 566..1183 /label=BBa_C0061 misc_feature 1184..1208 /label=Help:Barcodes CDS 566..1183 /label=BBa_C0061 misc_feature 548..1208 /label=BBa_C0261 misc_feature 1221..1224 /label=conserved RBS 1217..1228 /label=BBa_B0034 CDS 1238..1854 /label=tetR misc_feature 1855..1888 /label=SsrA misc_feature 1895..1919 /label=Help:Barcodes CDS 1235..1894 /label=BBa_C0040 stem_loop 1939..1982 /label=stem_loop misc_feature 1928..2007 /label=BBa_B0010 terminator 1928..2007 /label=BBa_B0010 stem_loop 2023..2042 /label=T7 TE misc_feature 2049..2049 /label=stop polyA_site 2043..2056 /label=polya misc_feature 2016..2056 /label=BBa_B0012 terminator 2016..2056 /label=BBa_B0012 misc_feature 2065..2083 /label=TetR 1 misc_feature 2065..2118 /label=BBa_R0040 promoter 2084..2089 /label=-35 misc_feature 2090..2108 /label=TetR 2 promoter 2107..2112 /label=-10 promoter 2065..2118 /label=BBa_R0040 misc_feature 1217..2118 /label=BBa_Q04400 misc_feature 2131..2134 /label=conserved RBS 2127..2138 /label=BBa_B0034 CDS 2148..2855 /label=cI lambda misc_feature 2856..2888 /label=LVA misc_feature 2895..2919 /label=Help:Barcodes CDS 2145..2894 /label=BBa_C0051 stem_loop 2939..2982 /label=stem_loop misc_feature 2928..3007 /label=BBa_B0010 terminator 2928..3007 /label=BBa_B0010 stem_loop 3023..3042 /label=T7 TE misc_feature 3049..3049 /label=stop polyA_site 3043..3056 /label=polya misc_feature 3016..3056 /label=BBa_B0012 terminator 3016..3056 /label=BBa_B0012 misc_feature 2127..3056 /label=BBa_P0451 misc_feature 1217..3056 /label=BBa_K376019 misc_feature 548..3056 /label=BBa_K376025 promoter 3065..3099 /label=BBa_J23116 misc_feature 3108..3906 /label=BBa_K376010 misc_feature 3065..3906 /label=BBa_K376013 misc_feature 548..3906 /label=BBa_K376029 ORIGIN 1 tatattgata aagatcaacc gctaaatatg cgttttgatg aagatcaact gatggctagc 61 tcagtcctag ggattatgct agctactaga ggaaattcac acaaatttaa ggagagtact 121 agatggcatc gttccaatcc ttcggcatcc caggtcaatt agaagtaatc aaaaaggctc 181 tggaccacgt ccgcgtgggc gtcgtgatca ccgatccggc gccggaggac aacccgatcg 241 tttacgtgaa tcaaggtttc gttcagatga cgggttacga aaccgaagag atcctgggca 301 aaaatgcgcg ttttctccag ggtaagcata cggacccggc ggaggttgat aacatccgta 361 ccgccttgca gaataaagag ccggttaccg tgcaaattca gaactataag aaagacggta 421 ccatgttttg gaacgagctg aacattgacc cgatggaaat tgaggacaag acttactttg 481 tcggcattca gaatgatatt acgaagcaga aagaatatga gaaactgctg gaatgataat 541 actagagaaa gaggagaaat actagatgac tataatgata aaaaaatcgg attttttggc 601 aattccatcg gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag 661 acttgagtgg gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa 721 tgcagaatat atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt 781 acctacaaca ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag 841 tgctcccaaa gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc 901 aaagataaat aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca 961 cgctgttagt caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt 1021 tttaaagcgt attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg 1081 tgatactaaa tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt 1141 aaatgctgca aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg 1201 tagatctcta ctagagaaag aggagaaata ctagatgtcc agattagata aaagtaaagt 1261 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa 1321 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg 1381 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc 1441 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc 1501 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca 1561 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa 1621 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca 1681 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt 1741 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg 1801 ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc 1861 aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt gtagatcact 1921 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 1981 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 2041 cctttctgcg tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt 2101 gatagagata ctgagcacta ctagagaaag aggagaaata ctagatgagc acaaaaaaga 2161 aaccattaac acaagagcag cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa 2221 agaaaaatga acttggctta tcccaggaat ctgtcgcaga caagatgggg atggggcagt 2281 caggcgttgg tgctttattt aatggcatca atgcattaaa tgcttataac gccgcattgc 2341 ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc agagaaatct 2401 acgagatgta tgaagcggtt agtatgcagc cgtcacttag aagtgagtat gagtaccctg 2461 ttttttctca tgttcaggca gggatgttct cacctgagct tagaaccttt accaaaggtg 2521 atgcggagag atgggtaagc acaaccaaaa aagccagtga ttctgcattc tggcttgagg 2581 ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc aagctttcct gacggaatgt 2641 taattctcgt tgaccctgag caggctgttg agccaggtga tttctgcata gccagacttg 2701 ggggtgatga gtttaccttc aagaaactga tcagggatag cggtcaggtg tttttacaac 2761 cactaaaccc acagtaccca atgatcccat gcaatgagag ttgttccgtt gtggggaaag 2821 ttatcgctag tcagtggcct gaagagacgt ttggcgctgc aaacgacgaa aactacgctt 2881 tagtagctta ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat 2941 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 3001 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 3061 agagttgaca gctagctcag tcctagggac tatgctagct actagagaaa gaggagaaat 3121 actagatgaa aaacataaat gccgacgaca catacagaat aattaataaa attaaagctt 3181 gtagaagcaa tattgatatt aatcaatgct tatctgatat gactaaaatg gtacattgtg 3241 aatattattt actcgcgatc atttatcctc attctatggt taaatctgat atttcaatcc 3301 tagataatta ccctaaaaaa tggaggcaat attatgatga cgctaattta ataaaatatg 3361 atcctatagt aggttattct aactccaatc attcaccaat taattggaat atatttgaaa 3421 acaatgctgt aaataaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat 3481 cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc 3541 acattcagaa aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc 3601 attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc 3661 aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag 3721 ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac 3781 caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt 3841 aacaggagca attgattgcc catactttaa aaattaataa cactgatagt gctagtgtag 3901 atcact //