LOCUS BBa_K379000 6457 bp DNA linear 22-Dec-2024 DEFINITION AHL induced concentration Band detect ACCESSION BBa_K379000 VERSION BBa_K379000.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1..1158 /label=BBa_F2621 misc_feature 1167..1178 /label=B0034 misc_feature 1185..1185 /label=P2 ogr CDS 1185..1403 /label=P2 ogr misc_feature 1167..1403 /label=BBa_I746350 stem_loop 1423..1466 /label=stem_loop misc_feature 1412..1491 /label=BBa_B0010 terminator 1412..1491 /label=BBa_B0010 stem_loop 1507..1526 /label=T7 TE misc_feature 1533..1533 /label=stop polyA_site 1527..1540 /label=polya misc_feature 1500..1540 /label=BBa_B0012 terminator 1500..1540 /label=BBa_B0012 terminator 1412..1540 /label=BBa_B0015 promoter 1549..1639 /label=PF promoter 1549..1639 /label=BBa_I746360 misc_feature 1652..1655 /label=conserved RBS 1648..1659 /label=BBa_B0034 CDS 1669..2376 /label=cI lambda misc_feature 2377..2409 /label=LVA misc_feature 2416..2440 /label=Help:Barcodes CDS 1666..2415 /label=BBa_C0051 stem_loop 2460..2503 /label=stem_loop misc_feature 2449..2528 /label=BBa_B0010 terminator 2449..2528 /label=BBa_B0010 stem_loop 2544..2563 /label=T7 TE misc_feature 2570..2570 /label=stop polyA_site 2564..2577 /label=polya misc_feature 2537..2577 /label=BBa_B0012 terminator 2537..2577 /label=BBa_B0012 promoter 2623..2628 /label=-10 promoter 2600..2605 /label=-35 misc_feature 2610..2626 /label=OR1 misc_feature 2586..2602 /label=OR2 misc_feature 2586..2634 /label=BBa_R0051 promoter 2586..2634 /label=BBa_R0051 misc_feature 1648..2634 /label=BBa_Q04510 misc_feature 2647..2650 /label=conserved RBS 2643..2654 /label=BBa_B0034 misc_feature 3750..3788 /label=LVA CDS 2661..3788 /label=lacI-LVA misc_feature 2661..3788 /label=BBa_C0012 misc_feature 3789..3813 /label=Help:Barcodes CDS 2661..3788 /label=BBa_C0012 stem_loop 3833..3876 /label=stem_loop misc_feature 3822..3901 /label=BBa_B0010 terminator 3822..3901 /label=BBa_B0010 stem_loop 3917..3936 /label=T7 TE misc_feature 3943..3943 /label=stop polyA_site 3937..3950 /label=polya misc_feature 3910..3950 /label=BBa_B0012 terminator 3910..3950 /label=BBa_B0012 misc_feature 2643..3950 /label=BBa_P0412 promoter 3959..4050 /label=PO promoter 3959..4050 /label=BBa_I746361 misc_feature 4063..4066 /label=conserved RBS 4059..4070 /label=BBa_B0034 misc_feature 5166..5204 /label=LVA CDS 4077..5204 /label=lacI-LVA misc_feature 4077..5204 /label=BBa_C0012 misc_feature 5205..5229 /label=Help:Barcodes CDS 4077..5204 /label=BBa_C0012 stem_loop 5249..5292 /label=stem_loop misc_feature 5238..5317 /label=BBa_B0010 terminator 5238..5317 /label=BBa_B0010 stem_loop 5333..5352 /label=T7 TE misc_feature 5359..5359 /label=stop polyA_site 5353..5366 /label=polya misc_feature 5326..5366 /label=BBa_B0012 terminator 5326..5366 /label=BBa_B0012 misc_feature 4059..5366 /label=BBa_P0412 CDS 5375..5462 /label=end of LacI coding region (inactive) misc_feature 5375..5574 /label=BBa_R0010 misc_feature 5463..5500 /label=CAP binding site promoter 5511..5516 /label=-35 promoter 5535..5540 /label=-10 misc_feature 5540..5574 /label=LacI binding site misc_feature 5547..5547 /label=start promoter 5375..5574 /label=BBa_R0010 misc_feature 5587..5590 /label=conserved RBS 5583..5594 /label=BBa_B0034 CDS 5601..6320 /label=GFP protein CDS 5601..6320 /label=BBa_E0040 stem_loop 6340..6383 /label=stem_loop misc_feature 6329..6408 /label=BBa_B0010 terminator 6329..6408 /label=BBa_B0010 stem_loop 6424..6443 /label=T7 TE misc_feature 6450..6450 /label=stop polyA_site 6444..6457 /label=polya misc_feature 6417..6457 /label=BBa_B0012 terminator 6417..6457 /label=BBa_B0012 terminator 6329..6457 /label=BBa_B0015 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgttt cattgtcctt 1201 tatgccagca tgccgcacat gcgcgtacaa gtcgctatat cactgacacg acaaaagagc 1261 gttatcatca gtgccagaac gtgaattgca gcgccacgtt catcacttat gagtcggtac 1321 agcgatacat cgtgaagccg ggagaagtcc acgccgtaag gccgcacccg ttgccatcag 1381 ggcagcaaat tatgtggatg taatactaga gccaggcatc aaataaaacg aaaggctcag 1441 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 1501 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtt gcccgccttt 1561 tctttaccgg tggttgtgct gtcgattagc caaccgggac aaatagcctg acatctccgg 1621 cgcaactgaa aataccactt actagagaaa gaggagaaat actagatgag cacaaaaaag 1681 aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa 1741 aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg gatggggcag 1801 tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa cgccgcattg 1861 cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc 1921 tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta tgagtaccct 1981 gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt taccaaaggt 2041 gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt ctggcttgag 2101 gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc tgacggaatg 2161 ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat agccagactt 2221 gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt gtttttacaa 2281 ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt tgtggggaaa 2341 gttatcgcta gtcagtggcc tgaagagacg tttggcgctg caaacgacga aaactacgct 2401 ttagtagctt aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa 2461 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 2521 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 2581 tagagtaaca ccgtgcgtgt tgactatttt acctctggcg gtgataatgg ttgctactag 2641 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 2701 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 2761 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 2821 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 2881 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 2941 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 3001 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 3061 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 3121 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 3181 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 3241 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 3301 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 3361 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 3421 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 3481 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 3541 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 3601 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 3661 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 3721 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 3781 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 3841 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 3901 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagcg 3961 cgccccgcga ttcgctaagg tgctgttgtg tcagtgataa gccatccggg actgatggcg 4021 gaggatgcgc atcgtcggga aactgatgcc tactagagaa agaggagaaa tactagatgg 4081 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga 4141 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg 4201 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca 4261 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa 4321 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg 4381 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg 4441 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg 4501 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta 4561 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc 4621 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg 4681 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg 4741 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc 4801 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg 4861 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca 4921 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa 4981 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt 5041 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc 5101 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 5161 ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt 5221 gtagatctct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 5281 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 5341 ttcgggtggg cctttctgcg tttatatact agagcaatac gcaaaccgcc tctccccgcg 5401 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 5461 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 5521 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacatactag 5581 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa 5641 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg 5701 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac 5761 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat 5821 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac 5881 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt 5941 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg 6001 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg 6061 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg 6121 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc 6181 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa 6241 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg 6301 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 6361 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 6421 actggctcac cttcgggtgg gcctttctgc gtttata //