LOCUS BBa_K379001 6472 bp DNA linear 26-Dec-2024 DEFINITION AHL induced concentration Band detect ACCESSION BBa_K379001 VERSION BBa_K379001.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1167..1178 /label=B0034 CDS 1188..1430 /label=phiR73 delta misc_feature 1188..1188 /label=phiR73 delta misc_feature 1268..1268 /label=silent mutation to remove PstI site misc_feature 1167..1430 /label=BBa_I746352 stem_loop 1450..1493 /label=stem_loop misc_feature 1439..1518 /label=BBa_B0010 terminator 1439..1518 /label=BBa_B0010 stem_loop 1534..1553 /label=T7 TE misc_feature 1560..1560 /label=stop polyA_site 1554..1567 /label=polya misc_feature 1527..1567 /label=BBa_B0012 terminator 1527..1567 /label=BBa_B0012 promoter 1576..1667 /label=PO promoter 1576..1667 /label=BBa_I746361 misc_feature 1680..1683 /label=conserved RBS 1676..1687 /label=BBa_B0034 CDS 1697..2404 /label=cI lambda misc_feature 2405..2437 /label=LVA misc_feature 2444..2468 /label=Help:Barcodes CDS 1694..2468 /label=BBa_C0051 stem_loop 2488..2531 /label=stem_loop misc_feature 2477..2556 /label=BBa_B0010 terminator 2477..2556 /label=BBa_B0010 stem_loop 2572..2591 /label=T7 TE misc_feature 2598..2598 /label=stop polyA_site 2592..2605 /label=polya misc_feature 2565..2605 /label=BBa_B0012 terminator 2565..2605 /label=BBa_B0012 promoter 2651..2656 /label=-10 promoter 2628..2633 /label=-35 misc_feature 2638..2654 /label=OR1 misc_feature 2614..2630 /label=OR2 misc_feature 2614..2662 /label=BBa_R0051 promoter 2614..2662 /label=BBa_R0051 misc_feature 2675..2678 /label=conserved RBS 2671..2682 /label=BBa_B0034 misc_feature 3778..3816 /label=LVA CDS 2689..3816 /label=lacI-LVA misc_feature 2689..3816 /label=BBa_C0012 misc_feature 3817..3841 /label=Help:Barcodes CDS 2689..3816 /label=BBa_C0012 stem_loop 3861..3904 /label=stem_loop misc_feature 3850..3929 /label=BBa_B0010 terminator 3850..3929 /label=BBa_B0010 stem_loop 3945..3964 /label=T7 TE misc_feature 3971..3971 /label=stop polyA_site 3965..3978 /label=polya misc_feature 3938..3978 /label=BBa_B0012 terminator 3938..3978 /label=BBa_B0012 promoter 3987..4079 /label=Psid promoter 3987..4079 /label=BBa_I746364 misc_feature 4092..4095 /label=conserved RBS 4088..4099 /label=BBa_B0034 misc_feature 5195..5233 /label=LVA CDS 4106..5233 /label=lacI-LVA misc_feature 4106..5233 /label=BBa_C0012 misc_feature 5234..5258 /label=Help:Barcodes CDS 4106..5233 /label=BBa_C0012 stem_loop 5278..5321 /label=stem_loop misc_feature 5267..5346 /label=BBa_B0010 terminator 5267..5346 /label=BBa_B0010 stem_loop 5362..5381 /label=T7 TE misc_feature 5388..5388 /label=stop polyA_site 5382..5395 /label=polya misc_feature 5355..5395 /label=BBa_B0012 terminator 5355..5395 /label=BBa_B0012 CDS 5404..5491 /label=end of LacI coding region (inactive) misc_feature 5404..5603 /label=BBa_R0010 misc_feature 5492..5529 /label=CAP binding site promoter 5540..5545 /label=-35 promoter 5564..5569 /label=-10 misc_feature 5569..5603 /label=LacI binding site misc_feature 5576..5576 /label=start promoter 5404..5603 /label=BBa_R0010 misc_feature 5616..5619 /label=conserved RBS 5612..5623 /label=BBa_B0034 CDS 5630..6304 /label=mrfp1 misc_feature 6311..6335 /label=Help:Barcodes CDS 5630..6335 /label=BBa_E1010 stem_loop 6355..6398 /label=stem_loop misc_feature 6344..6423 /label=BBa_B0010 terminator 6344..6423 /label=BBa_B0010 stem_loop 6439..6458 /label=T7 TE misc_feature 6465..6465 /label=stop polyA_site 6459..6472 /label=polya misc_feature 6432..6472 /label=BBa_B0012 terminator 6432..6472 /label=BBa_B0012 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgatg cgctgccctt 1201 tctgtcgtca ttcagcgcat acccgcacca gccggtatgt gagtgacaat gtcaaagaaa 1261 gttatctcca gtgccagaat atttactgtt cggcgacatt taaaacgcat gagtcaattt 1321 gtgccgtgat tcgttctccg gtcacggagg aaaaaccagc accggcaagc acagcaccgg 1381 ctgttgtccg aaaagttaaa ggctgttaca gctcaccatt caaccattaa tactagagcc 1441 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 1501 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 1561 gtttatatac tagagcgcgc cccgcgattc gctaaggtgc tgttgtgtca gtgataagcc 1621 atccgggact gatggcggag gatgcgcatc gtcgggaaac tgatgcctac tagagaaaga 1681 ggagaaatac tagatgagca caaaaaagaa accattaaca caagagcagc ttgaggacgc 1741 acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa cttggcttat cccaggaatc 1801 tgtcgcagac aagatgggga tggggcagtc aggcgttggt gctttattta atggcatcaa 1861 tgcattaaat gcttataacg ccgcattgct tgcaaaaatt ctcaaagtta gcgttgaaga 1921 atttagccct tcaatcgcca gagaaatcta cgagatgtat gaagcggtta gtatgcagcc 1981 gtcacttaga agtgagtatg agtaccctgt tttttctcat gttcaggcag ggatgttctc 2041 acctgagctt agaaccttta ccaaaggtga tgcggagaga tgggtaagca caaccaaaaa 2101 agccagtgat tctgcattct ggcttgaggt tgaaggtaat tccatgaccg caccaacagg 2161 ctccaagcca agctttcctg acggaatgtt aattctcgtt gaccctgagc aggctgttga 2221 gccaggtgat ttctgcatag ccagacttgg gggtgatgag tttaccttca agaaactgat 2281 cagggatagc ggtcaggtgt ttttacaacc actaaaccca cagtacccaa tgatcccatg 2341 caatgagagt tgttccgttg tggggaaagt tatcgctagt cagtggcctg aagagacgtt 2401 tggcgctgca aacgacgaaa actacgcttt agtagcttaa taacgctgat agtgctagtg 2461 tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 2521 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 2581 tcgggtgggc ctttctgcgt ttatatacta gagtaacacc gtgcgtgttg actattttac 2641 ctctggcggt gataatggtt gctactagag aaagaggaga aatactagat ggtgaatgtg 2701 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc 2761 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg 2821 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg 2881 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg 2941 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga 3001 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg 3061 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact 3121 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc 3181 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa 3241 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg 3301 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt 3361 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg 3421 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg 3481 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt 3541 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg 3601 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc 3661 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg 3721 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggct 3781 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 3841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 3901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 3961 ggcctttctg cgtttatata ctagagtggc ttgccggtct gaggatgagt ctcctgtgtc 4021 agggctggca catctgcaat gcgtcgtgtt gttgtccggt gtacgtcaca attttcttat 4081 actagagaaa gaggagaaat actagatggt gaatgtgaaa ccagtaacgt tatacgatgt 4141 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca 4201 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 4261 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 4321 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 4381 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc 4441 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga 4501 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga 4561 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 4621 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt 4681 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 4741 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 4801 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat 4861 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc 4921 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 4981 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca 5041 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 5101 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 5161 ggcacgacag gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt 5221 agtagcttaa taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata 5281 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 5341 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 5401 gagcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 5461 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 5521 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 5581 tgagcggata acaatttcac acatactaga gaaagaggag aaatactaga tggcttcctc 5641 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg 5701 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc 5761 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca 5821 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa 5881 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt 5941 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact 6001 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga 6061 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg 6121 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa 6181 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca 6241 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg 6301 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa 6361 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 6421 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //