LOCUS       BBa_K381001              986 bp    DNA     linear       22-Dec-2024
DEFINITION  Nitrate reporter: PyeaR - GFP composite
ACCESSION   BBa_K381001
VERSION     BBa_K381001.1  
FEATURES             Location/Qualifiers
     misc_feature    50..65
                     /label=NarL-binding region
     misc_feature    58..80
                     /label=NsrR-binding region
     misc_feature    66..71
                     /label=promoter -35 region
     misc_feature    89..94
                     /label=promoter -10 region
     promoter        1..100
                     /label=BBa_K216005
     RBS             109..123
                     /label=RBS-1\Strong
     RBS             116..120
                     /label=RBS
     misc_feature    109..123
                     /label=BBa_B0030
     RBS             109..123
                     /label=BBa_B0030
     CDS             130..849
                     /label=GFP protein
     CDS             130..849
                     /label=BBa_E0040
     stem_loop       869..912
                     /label=stem_loop
     misc_feature    858..937
                     /label=BBa_B0010
     terminator      858..937
                     /label=BBa_B0010
     stem_loop       953..972
                     /label=T7 TE
     misc_feature    979..979
                     /label=stop
     polyA_site      973..986
                     /label=polya
     misc_feature    946..986
                     /label=BBa_B0012
     terminator      946..986
                     /label=BBa_B0012
ORIGIN
        1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa
       61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg tactagagat taaagaggag
      121 aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
      181 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
      241 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
      301 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat
      361 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
      421 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
      481 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
      541 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
      601 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
      661 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
      721 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
      781 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
      841 aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      901 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      961 ttcgggtggg cctttctgcg tttata
//