LOCUS       BBa_K387010             2504 bp    DNA     linear       01-Jun-2024
DEFINITION  RBS34-SoxR-dT-PsoxS-RBS34-luciferase-dt
ACCESSION   BBa_K387010
VERSION     BBa_K387010.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     stem_loop       501..520
                     /label=T7 TE
     misc_feature    527..527
                     /label=stop
     polyA_site      521..534
                     /label=polya
     misc_feature    494..534
                     /label=BBa_B0012
     terminator      494..534
                     /label=BBa_B0012
     stem_loop       555..577
                     /label=stem_loop
     misc_feature    543..588
                     /label=BBa_B0011
     terminator      543..588
                     /label=BBa_B0011
     misc_feature    5..8
                     /label=conserved
     misc_feature    1..12
                     /label=BBa_B0034
     misc_feature    19..485
                     /label=BBa_K387002
     misc_feature    494..534
                     /label=BBa_B0012
     stem_loop       501..520
                     /label=T7 TE
     polyA_site      521..534
                     /label=polya
     misc_feature    527..527
                     /label=stop
     misc_feature    543..588
                     /label=BBa_B0011
     stem_loop       555..577
                     /label=stem_loop
     misc_feature    597..681
                     /label=BBa_K387001
     promoter        1..722
                     /label=BBa_K387004
     misc_feature    735..738
                     /label=conserved
     RBS             731..742
                     /label=BBa_B0034
     CDS             749..2401
                     /label=BBa_I712019
     stem_loop       2417..2436
                     /label=T7 TE
     misc_feature    2443..2443
                     /label=stop
     polyA_site      2437..2450
                     /label=polya
     misc_feature    2410..2450
                     /label=BBa_B0012
     terminator      2410..2450
                     /label=BBa_B0012
     stem_loop       2471..2493
                     /label=stem_loop
     misc_feature    2459..2504
                     /label=BBa_B0011
     terminator      2459..2504
                     /label=BBa_B0011
ORIGIN
        1 aaagaggaga aatactagag ttatggaaaa gaaattaccc cgcattaaag cgctgctaac
       61 ccccggcgaa gtggcgaaac gcagcggtgt ggcggtatcg gcgctgcatt tctatgaaag
      121 taaagggttg attaccagta tccgtaacag cggcaatcag cggcgatata aacgtgatgt
      181 gttgcgatat gttgcaatta tcaaaattgc tcagcgtatt ggcattccgc tggcgaccat
      241 tggtgaagcg tttggcgtgt tgcccgaagg gcatacgtta agtgcgaaag agtggaaaca
      301 gctttcgtcc caatggcgag aagagttgga tcggcgcatt cataccttag tggcgctgcg
      361 tgacgaactg gacggatgta ttggttgtgg ctgcctttcg cgcagtgatt gcccgttgcg
      421 taacccgggc gaccgcttag gagaagaagg taccggcgca cgcttgctgg aagatgaaca
      481 aaactaaagc gccacaaggg cgctttagtt tgttttccgg tctttgtact agagtcacac
      541 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc
      601 agattattaa tccggctttt ttattatttt actagagaaa tctgcctctt ttcagtgttc
      661 agttcgttaa ttcatctgtt ggggagtata attcctcaag ttaacttgag gtaaagcgat
      721 tttactagag aaagaggaga aatactagat ggaagacgcc aaaaacataa agaaaggccc
      781 ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa
      841 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat
      901 cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg
      961 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc
     1021 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga
     1081 acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa
     1141 ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat
     1201 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca
     1261 tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac
     1321 aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc
     1381 tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat
     1441 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac
     1501 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga
     1561 gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct
     1621 attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga
     1681 aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt
     1741 ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat
     1801 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc
     1861 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg
     1921 tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
     1981 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
     2041 cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc
     2101 cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg
     2161 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
     2221 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
     2281 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
     2341 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta
     2401 atactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag
     2461 agaatataaa aagccagatt attaatccgg cttttttatt attt
//