BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23110 1 BBa_J23110 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K389001 1 virA VirA receptor 2010-08-09T11:00:00Z 2015-05-08T01:12:18Z ''Agrobacterium tumefaciens'' C58, TI-plasmid This is a gene for the ''virA'' receptor from ''Agrobacterium tumefaciens'' C58. An illegal ''PstI'' restriction site was removed by site-directed mutagenesis. false false _517_ 0 6670 9 It's complicated false Illegal ''PstI'' restriction site removed false Jonas Aretz annotation2077044 1 virA range2077044 1 1 2505 annotation2078363 1 Stop codon range2078363 1 2503 2505 annotation2077043 1 Illegal PstI site removed range2077043 1 187 187 BBa_K389010 1 virA VirA receptor (expressed) 2010-08-09T11:00:00Z 2015-05-08T01:12:19Z ''A. tumefaciens'' C58, partsregistry This BioBrick contains a ''virA'' receptor from ''Agrobacterium tumefaciens'' C58 under the control of a constitutive promotor. We used it to mutate ''virA'' by error-prone PCR. For screening the mutants BBa_K389011 is also needed. false false _517_ 0 6670 9 It's complicated false The ''virA'' gene is under the control a medium strong constitutive promotor. false Jonas Aretz component2219529 1 BBa_B0034 component2219533 1 BBa_K389001 component2219527 1 BBa_J23110 annotation2219533 1 BBa_K389001 range2219533 1 62 2566 annotation2219529 1 BBa_B0034 range2219529 1 44 55 annotation2219527 1 BBa_J23110 range2219527 1 1 35 BBa_K389010_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaatggaaggtattcaccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgccttgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacagtgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttgggagctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaactgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgcaagattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcgaacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcgacatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgattttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaagtgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggcctttgcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggtaatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgcttggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaagtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatgagcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctgctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccaggcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcgatattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttcaacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagacacattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactagaacgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaacatcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatgaatctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcgacagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcattgccgaaactgtgctcccccacatttttgagcctttctttacaacacgctcttgcagcggcggcacgggtctaggtcttgctgccgtgcacggtcatgtcagcgcgcttgcaggatatattgacgttacttcagccgtagggcgagggacgcgcttcgacatttatctacctccttcctcgaagaagcccgtcagcccggacgcgttttttgggccctgtaaaacaccgcgtggcaacggagaaattgtggcattgattgagccagatcctgtcttgcgggaggtgtacgaagacaagatcgccgctctgggctatgagccggtgggctttaagacatgtgcagacctttgcaattggatatcaaaaggcaagcaagccgatctggttctagttgaccaatcgtctcttcccgagaatcagagtgctactgctttgcacgcagccttcaagacggcgtccatcatcattggaggaagtgatcttaaaatgtcactttccagcgatgacatgacgtccgcgctttttctgcccaaacctatatcctccaggacgatggcctacgcgattcgtaccaagatcaaagcctaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_J23110_sequence 1 tttacggctagctcagtcctaggtacaatgctagc BBa_K389001_sequence 1 atgaatggaaggtattcaccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgccttgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacagtgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttgggagctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaactgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgcaagattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcgaacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcgacatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgattttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaagtgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggcctttgcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggtaatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgcttggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaagtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatgagcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctgctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccaggcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcgatattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttcaacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagacacattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactagaacgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaacatcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatgaatctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcgacagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcattgccgaaactgtgctcccccacatttttgagcctttctttacaacacgctcttgcagcggcggcacgggtctaggtcttgctgccgtgcacggtcatgtcagcgcgcttgcaggatatattgacgttacttcagccgtagggcgagggacgcgcttcgacatttatctacctccttcctcgaagaagcccgtcagcccggacgcgttttttgggccctgtaaaacaccgcgtggcaacggagaaattgtggcattgattgagccagatcctgtcttgcgggaggtgtacgaagacaagatcgccgctctgggctatgagccggtgggctttaagacatgtgcagacctttgcaattggatatcaaaaggcaagcaagccgatctggttctagttgaccaatcgtctcttcccgagaatcagagtgctactgctttgcacgcagccttcaagacggcgtccatcatcattggaggaagtgatcttaaaatgtcactttccagcgatgacatgacgtccgcgctttttctgcccaaacctatatcctccaggacgatggcctacgcgattcgtaccaagatcaaagcctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z