LOCUS       BBa_K389012             2787 bp    DNA     linear       25-Nov-2024
DEFINITION  VirA reporter system luc
ACCESSION   BBa_K389012
VERSION     BBa_K389012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     RBS             47..51
                     /label=RBS
     CDS             55..780
                     /label=virG
     misc_feature    781..783
                     /label=Stop codon
     CDS             44..783
                     /label=BBa_K389002
     stem_loop       803..846
                     /label=stem_loop
     misc_feature    792..871
                     /label=BBa_B0010
     terminator      792..871
                     /label=BBa_B0010
     stem_loop       891..934
                     /label=stem_loop
     misc_feature    880..959
                     /label=BBa_B0010
     terminator      880..959
                     /label=BBa_B0010
     misc_feature    1000..1011
                     /label=vir-box
     RBS             1120..1124
                     /label=rbs
     promoter        1056..1060
                     /label=-35
     promoter        1080..1084
                     /label=-10
     promoter        968..1125
                     /label=BBa_K389003
     CDS             1132..2784
                     /label=Luciferase
     misc_feature    2785..2787
                     /label=Stop codon
     misc_feature    1132..2787
                     /label=BBa_K389004
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaagagga gaaaatgaaa
       61 cacgtcctgc tggttgatga tgatgtggct atgcgtcacc tgattatcga gtatctgacc
      121 atccatgcct tcaaagtcac agccgttgcc gattccactc agtttacccg tgtactgtct
      181 agtgctaccg tcgatgttgt cgtggtcgat ctgaatctgg ttcgtgaaga tggcctggaa
      241 attgtccgta acctggcagc aaaaagcgat atccctatca ttgtgatcag cggggatcgt
      301 ctggaggaaa cagacaaagt tgttgccctg gagctgggtg cttctgattt tattgccaaa
      361 ccgttcagca tccgtgagtt tctggcccgt attcgtgtag ccctgcgcgt tcgtcctaac
      421 gtcgttcgtt cgaaagaccg tcgttccttt tgttttaccg actggaccct gaatctgcgt
      481 caacgtcgcc tgatgagcga agctggcggt gaagtgaaac tgacagccgg tgagttcaat
      541 ctgctgctgg catttctgga aaaacctcgt gatgttctga gccgtgaaca gctgctgatt
      601 gcctctcgtg tccgtgatga agaggtgtat gatcgttcta tcgatgtcct gattctgcgc
      661 ctgcgtcgca aactggaagc cgatccgtct agtcctcagc tgattaaaac cgcccgtggt
      721 gctgggtatt tcttcgatgc cgatgtccaa gtgtcacatg gtggcacgat ggctgcttaa
      781 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      841 ttttatctgt tgtttgtcgg tgaacgctct ctactagagc caggcatcaa ataaaacgaa
      901 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      961 actagaggca cccttaagtg catggaaagc cgttttcgct tcaaatgaaa tcgaaaagaa
     1021 gaaaacaaaa atcctagagt aaccgaccct cccgataatc gtgaacatca gatgacagca
     1081 tttcttccga ccgaagtggc tgtgttggtt atgagcttga ggagatacta gatggaagat
     1141 gccaaaaaca ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc
     1201 gagcagctgc acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc
     1261 gacgcacata tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg
     1321 gcagaagcta tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag
     1381 aatagcttgc agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc
     1441 ccagctaacg acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc
     1501 accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta
     1561 ccgatcatac aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc
     1621 atgtacacct tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc
     1681 gagagcttcg accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga
     1741 ttgcccaagg gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc
     1801 gaccccatct tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt
     1861 caccacggct tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg
     1921 ctcatgtacc gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa
     1981 tctgccctgc tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag
     2041 tacgacctaa gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta
     2101 ggtgaggccg tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca
     2161 gaaacaacca gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc
     2221 aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt
     2281 gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac
     2341 aaccccgagg ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc
     2401 gcctactggg acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa
     2461 tacaagggct accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac
     2521 atcttcgacg ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca
     2581 gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc
     2641 agccaggtta caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct
     2701 aaaggactga ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag
     2761 aagggcggca agatcgccgt gtaataa
//