LOCUS       BBa_K389013             1837 bp    DNA     linear       09-May-2024
DEFINITION  VirA reporter system mRFP
ACCESSION   BBa_K389013
VERSION     BBa_K389013.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     RBS             47..51
                     /label=RBS
     CDS             55..780
                     /label=virG
     misc_feature    781..783
                     /label=Stop codon
     CDS             44..783
                     /label=BBa_K389002
     stem_loop       803..846
                     /label=stem_loop
     misc_feature    792..871
                     /label=BBa_B0010
     terminator      792..871
                     /label=BBa_B0010
     stem_loop       891..934
                     /label=stem_loop
     misc_feature    880..959
                     /label=BBa_B0010
     terminator      880..959
                     /label=BBa_B0010
     terminator      792..959
                     /label=BBa_B0017
     misc_feature    1000..1011
                     /label=vir-box
     RBS             1120..1124
                     /label=rbs
     promoter        1056..1060
                     /label=-35
     promoter        1080..1084
                     /label=-10
     promoter        968..1125
                     /label=BBa_K389003
     CDS             1132..1806
                     /label=mrfp1
     misc_feature    1813..1837
                     /label=Help:Barcodes
     CDS             1132..1837
                     /label=BBa_E1010
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaagagga gaaaatgaaa
       61 cacgtcctgc tggttgatga tgatgtggct atgcgtcacc tgattatcga gtatctgacc
      121 atccatgcct tcaaagtcac agccgttgcc gattccactc agtttacccg tgtactgtct
      181 agtgctaccg tcgatgttgt cgtggtcgat ctgaatctgg ttcgtgaaga tggcctggaa
      241 attgtccgta acctggcagc aaaaagcgat atccctatca ttgtgatcag cggggatcgt
      301 ctggaggaaa cagacaaagt tgttgccctg gagctgggtg cttctgattt tattgccaaa
      361 ccgttcagca tccgtgagtt tctggcccgt attcgtgtag ccctgcgcgt tcgtcctaac
      421 gtcgttcgtt cgaaagaccg tcgttccttt tgttttaccg actggaccct gaatctgcgt
      481 caacgtcgcc tgatgagcga agctggcggt gaagtgaaac tgacagccgg tgagttcaat
      541 ctgctgctgg catttctgga aaaacctcgt gatgttctga gccgtgaaca gctgctgatt
      601 gcctctcgtg tccgtgatga agaggtgtat gatcgttcta tcgatgtcct gattctgcgc
      661 ctgcgtcgca aactggaagc cgatccgtct agtcctcagc tgattaaaac cgcccgtggt
      721 gctgggtatt tcttcgatgc cgatgtccaa gtgtcacatg gtggcacgat ggctgcttaa
      781 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      841 ttttatctgt tgtttgtcgg tgaacgctct ctactagagc caggcatcaa ataaaacgaa
      901 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      961 actagaggca cccttaagtg catggaaagc cgttttcgct tcaaatgaaa tcgaaaagaa
     1021 gaaaacaaaa atcctagagt aaccgaccct cccgataatc gtgaacatca gatgacagca
     1081 tttcttccga ccgaagtggc tgtgttggtt atgagcttga ggagatacta gatggcttcc
     1141 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
     1201 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
     1261 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
     1321 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
     1381 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
     1441 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
     1501 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
     1561 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
     1621 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
     1681 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
     1741 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
     1801 ggtgcttaat aacgctgata gtgctagtgt agatcgc
//