BBa_K389003 1 Pvir virB promoter 2010-08-09T11:00:00Z 2015-05-08T01:12:19Z ''A. tumefaciens'' C58, TI-plasmid This is a ''vir'' promotor from ''Agrobacterium tumefaciens'' C58. It is located on the TI-plasmid before the ''virB'' genes. It is activated by phosphorylated ''virG'' response regulator. false false _517_ 0 6670 9 It's complicated false contains already a RBS false Jonas Aretz annotation2077850 1 -35 range2077850 1 89 93 annotation2077848 1 vir-box range2077848 1 33 44 annotation2077849 1 rbs range2077849 1 153 157 annotation2077851 1 -10 range2077851 1 113 117 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation2214014 1 Help:Barcodes range2214014 1 682 706 annotation1014044 1 mrfp1 range1014044 1 1 675 BBa_B0017 1 BBa_B0017 double terminator (B0010-B0010) 2004-01-08T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator with two copies of <bb_part>BBa_B0010</bb_part> false false _1_ 0 24 7 In stock false true Austin Che component939331 1 BBa_B0010 component939337 1 BBa_B0010 annotation939337 1 BBa_B0010 range939337 1 89 168 annotation939331 1 BBa_B0010 range939331 1 1 80 BBa_J23110 1 BBa_J23110 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K389013 1 BBa_K389013 VirA reporter system mRFP 2010-09-10T11:00:00Z 2015-05-08T01:12:19Z partsregistry, synthetic gene by Mr. Gene, TI-plasmid from ''Agrobacterium tumefaciens'' C58 With this system it is possible to measure the activity of a virA receptor and the vir promoter, respectively. The reporter is the mRFP from the partsregistry. false false _517_ 0 6670 9 It's complicated false medium strong constitutive promotor, terminator to keep basal expression of mRFP low false Jonas Aretz component2263596 1 BBa_E1010 component2263588 1 BBa_B0017 component2263579 1 BBa_J23110 component2263593 1 BBa_K389003 component2263583 1 BBa_K389002 annotation2263588 1 BBa_B0017 range2263588 1 792 959 annotation2263579 1 BBa_J23110 range2263579 1 1 35 annotation2263583 1 BBa_K389002 range2263583 1 44 783 annotation2263596 1 BBa_E1010 range2263596 1 1132 1837 annotation2263593 1 BBa_K389003 range2263593 1 968 1125 BBa_K389002 1 virG Mutated virG 2010-08-09T11:00:00Z 2015-05-08T01:12:18Z synthetic This is the ''virG'' response regulator from the virA/G receptor system. The virA/G receptor system is used by ''Agrobacterium tumefaciens'' to detect phenolic substances secreted by plants. In presence of these substances, ''virG'' is activated by the ''virA'' receptor and induces the transcription of genes under the control of a ''vir'' promotor. Normally, ''virG'' needs the ''rpoA'' RNA polymerase subunit from ''A. tumefaciens'' to work in ''Escherichia coli'' but this ''virG'' BioBrick is mutated, so works with the ''rpoA'' subunit from ''E. coli''. false false _517_ 0 6670 9 It's complicated false synthesized by Mr. Gene, so optimated codon usage, removal of illegal restriction sites false Jonas Aretz annotation2078361 1 virG range2078361 1 12 737 annotation2078362 1 Stop codon range2078362 1 738 740 annotation2078360 1 RBS range2078360 1 4 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K389002_sequence 1 aagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataa BBa_B0017_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K389003_sequence 1 gcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggaga BBa_K389013_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagaagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagaggcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggagatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_J23110_sequence 1 tttacggctagctcagtcctaggtacaatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z