LOCUS BBa_K389014 4524 bp DNA linear 22-Nov-2024 DEFINITION VirA/G screening system test ACCESSION BBa_K389014 VERSION BBa_K389014.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 248..248 /label=Illegal PstI site removed CDS 62..2566 /label=virA misc_feature 2564..2566 /label=Stop codon CDS 62..2566 /label=BBa_K389001 misc_feature 1..2566 /label=BBa_K389010 promoter 2575..2609 /label=BBa_J23110 RBS 2621..2625 /label=RBS CDS 2629..3354 /label=virG misc_feature 3355..3357 /label=Stop codon CDS 2618..3357 /label=BBa_K389002 stem_loop 3377..3420 /label=stem_loop misc_feature 3366..3445 /label=BBa_B0010 terminator 3366..3445 /label=BBa_B0010 stem_loop 3465..3508 /label=stem_loop misc_feature 3454..3533 /label=BBa_B0010 terminator 3454..3533 /label=BBa_B0010 misc_feature 3574..3585 /label=vir-box RBS 3694..3698 /label=rbs promoter 3630..3634 /label=-35 promoter 3654..3658 /label=-10 promoter 3542..3699 /label=BBa_K389003 CDS 3706..4521 /label=kanamycin resistance misc_feature 4522..4524 /label=Stop codon CDS 3706..4524 /label=BBa_K389005 misc_feature 2575..4524 /label=BBa_K389011 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgaatgga aggtattcac cgtctcggca agatttcaag acaggcgcca agccttggtc 121 tatcctggcc ttggtagttg ctgcaatgat ttttgccttg atggcgatta cgtcttggca 181 ggacaatgaa accaatcggg cgatcctgac ccaattgcga gctattaaca tcgacagtgc 241 ttcgctccag cgggatgtac tccgcgcgga agcgggtgtg gtggcgaact accggcccat 301 tatctccagg ttgggagctt tgcggaagaa cctggaaaat ttgaagcgac tatttaaaca 361 atctcatctt gtgatcggca atgatttctc tcaactgctc gacaagctaa aggtgtctgt 421 ggatacgacc gacgcggccg ttgcagcctt cggagcgcaa aacgtgctcc tgcaagattc 481 gcttgccagc ttcactcgcg cgcttagtat tcttcccaaa atgtcgtcga cggatcagac 541 ggtcgaaaat tcgaacgaat tgggcagcct gatgctgcgg tttgtgcgtc agccaagccc 601 agcactctcg ttggagatca gtcacgaact cgacatgctc caaaaagcta gcggtggggc 661 tgaagttcct atccgtatac ttgcacgcga gggtcgcgtc atcttgtcga ttttgccccg 721 tgtgaacgat gccgtaaaca tgattcagac ctccgacacc gctgaaattg ccgaaagatt 781 ggagcgcaag tgtttggagg cctatagctt gcaaagcgtg agggagcagc gggcacggat 841 cttcttgggt tccgtttcgg tgggcctttg catctacatc atctcactgg tctataggct 901 gcgtcggaaa acggcttggt taacgcggcg tttggattac gaagaggtaa tcaaagagat 961 tggggtttgt ttcgaggggg gaggggccac agcgtcgtcc ctcaattcgt ccgcgcaagc 1021 tgcgcttgga attattcaac gcttctttaa tgcggaatcg tgtgcactag cattggtgga 1081 ccatggtgac aggtgggctg tcgaaagttt cgctgcgaag ctgcctgagc ccgtctggga 1141 ggacctcgcg ctacgcgaga tggtttctct tgccagagcg gatgagcgtg cgtcagtatt 1201 ccgcatcatg tcgacgcgaa aggtcagctg ccttcctccg gagactccgg gcgtttctat 1261 gctgctggca cacaaatcta cggatcaact gatagcgatt tgttccctcg gttaccaggg 1321 ctatcgtctg aaatcttgtc caggcgaagt tcagcttctt gaactcgcca ccgcctgcct 1381 ctgccactat atcgatgtcc ggcgtaagca gaccgaatgc gatattctgg agaggcgatt 1441 agagcatgcg gaacgtcttc aggcagttgg tacacttgct ggtggaatag cgcatgagtt 1501 caacaacatt ttgggagcaa tcctcgggta cgctgaaatg gcgcaaaaca tgctgcgtcg 1561 gtcatctgtc acccgaagac acattgacca aattatttcg tcgggtgaca gagccaggct 1621 cattatcgat cagatcttga ctctaagtcg aaaactagaa cgcgtgacaa agccgttcag 1681 tgtctccgaa ctcgtaatgg aaattgctcc cttattgcgt gttgcattgc agcgcaacat 1741 cgagctgaag ttcaagtttg acgacaagaa gagcgtagtc gaaggaagcc cgctagaggt 1801 tcagcagatg ctgatgaatc tttgcaagaa cgcttcccag gcgtttaccg ccgatggtca 1861 aatcgacatc atcgttagcc gaatttttgt atctcgacag aaagtactgg cgcatggtgt 1921 tatgccagct ggcgactatg ttcttctttc tgtcagcgac gacggtgaag gcattgccga 1981 aactgtgctc ccccacattt ttgagccttt ctttacaaca cgctcttgca gcggcggcac 2041 gggtctaggt cttgctgccg tgcacggtca tgtcagcgcg cttgcaggat atattgacgt 2101 tacttcagcc gtagggcgag ggacgcgctt cgacatttat ctacctcctt cctcgaagaa 2161 gcccgtcagc ccggacgcgt tttttgggcc ctgtaaaaca ccgcgtggca acggagaaat 2221 tgtggcattg attgagccag atcctgtctt gcgggaggtg tacgaagaca agatcgccgc 2281 tctgggctat gagccggtgg gctttaagac atgtgcagac ctttgcaatt ggatatcaaa 2341 aggcaagcaa gccgatctgg ttctagttga ccaatcgtct cttcccgaga atcagagtgc 2401 tactgctttg cacgcagcct tcaagacggc gtccatcatc attggaggaa gtgatcttaa 2461 aatgtcactt tccagcgatg acatgacgtc cgcgcttttt ctgcccaaac ctatatcctc 2521 caggacgatg gcctacgcga ttcgtaccaa gatcaaagcc taataatact agagtttacg 2581 gctagctcag tcctaggtac aatgctagct actagagaag aggagaaaat gaaacacgtc 2641 ctgctggttg atgatgatgt ggctatgcgt cacctgatta tcgagtatct gaccatccat 2701 gccttcaaag tcacagccgt tgccgattcc actcagttta cccgtgtact gtctagtgct 2761 accgtcgatg ttgtcgtggt cgatctgaat ctggttcgtg aagatggcct ggaaattgtc 2821 cgtaacctgg cagcaaaaag cgatatccct atcattgtga tcagcgggga tcgtctggag 2881 gaaacagaca aagttgttgc cctggagctg ggtgcttctg attttattgc caaaccgttc 2941 agcatccgtg agtttctggc ccgtattcgt gtagccctgc gcgttcgtcc taacgtcgtt 3001 cgttcgaaag accgtcgttc cttttgtttt accgactgga ccctgaatct gcgtcaacgt 3061 cgcctgatga gcgaagctgg cggtgaagtg aaactgacag ccggtgagtt caatctgctg 3121 ctggcatttc tggaaaaacc tcgtgatgtt ctgagccgtg aacagctgct gattgcctct 3181 cgtgtccgtg atgaagaggt gtatgatcgt tctatcgatg tcctgattct gcgcctgcgt 3241 cgcaaactgg aagccgatcc gtctagtcct cagctgatta aaaccgcccg tggtgctggg 3301 tatttcttcg atgccgatgt ccaagtgtca catggtggca cgatggctgc ttaataatac 3361 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 3421 ctgttgtttg tcggtgaacg ctctctacta gagccaggca tcaaataaaa cgaaaggctc 3481 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 3541 ggcaccctta agtgcatgga aagccgtttt cgcttcaaat gaaatcgaaa agaagaaaac 3601 aaaaatccta gagtaaccga ccctcccgat aatcgtgaac atcagatgac agcatttctt 3661 ccgaccgaag tggctgtgtt ggttatgagc ttgaggagat actagatgag ccatattcaa 3721 cgggaaacgt cttgctcccg tccgcgctta aactccaaca tggacgctga tttatatggg 3781 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg 3841 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt 3901 acagatgaga tggtccgtct caactggctg acggagttta tgcctctccc gaccatcaag 3961 cattttatcc gtactcctga tgatgcgtgg ttactcacca ccgcgattcc tgggaaaaca 4021 gccttccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggcc 4081 gtgttcctgc gccggttaca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgt 4141 gtatttcgtc ttgctcaggc gcaatcacgc atgaataacg gtttggttga tgcgagtgat 4201 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcacaagctc 4261 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt 4321 tttgacgagg ggaaattaat aggttgtatt gatgttggac gggtcggaat cgcagaccgt 4381 taccaggacc ttgccattct ttggaactgc ctcggtgagt tttctccttc attacagaaa 4441 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg 4501 atgctcgatg agtttttcta ataa //