BBa_K389014
type: ComponentDefinition
persistent identity: BBa K389014
display id: BBa_K389014
version: 1
was generated by: iGEM to SBOL conversion
description: VirA/G screening system test
created: 2010-09-27T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: ''A. tumefaciens'' C58 TI-plasmid, partsregistry, Mr. Gene
owned by: james
owned by: myers
mutable description: This BioBrick is made to test our ''virA'' screening system and the strategy for directed evolution, respectively. It contains an unmutated ''virA'' under the control of a constitutive promoter and the screening device <partinfo>K389011</partinfo>.
discontinued: false
dominant: false
experience: experience/None
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: medium strong constitutive promoters, only for testing the screening principle of mutated ''virA'' screening
star: false
creator: Jonas Aretz
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K389010
component: BBa_K389011
sequence annotation: BBa_K389010
sequence annotation: BBa_K389011
sequence: https://synbiohub.org/public/igem/BBa_K389014_sequence/1

BBa_K389010
type: Component
persistent identity: component2227607
display id: component2227607
version: 1
top level: BBa_K389014
definition: virA
access: public

BBa_K389011
type: Component
persistent identity: component2227625
display id: component2227625
version: 1
top level: BBa_K389014
definition: BBa_K389011
access: public

BBa_K389010
type: SequenceAnnotation
persistent identity: annotation2227607
display id: annotation2227607
version: 1
top level: BBa_K389014
location: https://synbiohub.org/public/igem/BBa_K389014/annotation2227607/range2227607/1
component: BBa_K389010

https://synbiohub.org/public/igem/BBa_K389014/annotation2227607/range2227607/1
type: Range
persistent identity: range2227607
display id: range2227607
version: 1
top level: BBa_K389014
direction: direction/reverse
start: 1
end: 2566
orientation: inline

BBa_K389011
type: SequenceAnnotation
persistent identity: annotation2227625
display id: annotation2227625
version: 1
top level: BBa_K389014
location: https://synbiohub.org/public/igem/BBa_K389014/annotation2227625/range2227625/1
component: BBa_K389011

https://synbiohub.org/public/igem/BBa_K389014/annotation2227625/range2227625/1
type: Range
persistent identity: range2227625
display id: range2227625
version: 1
top level: BBa_K389014
direction: direction/reverse
start: 2575
end: 4524
orientation: inline

virA
type: ComponentDefinition
persistent identity: BBa K389010
display id: BBa_K389010
version: 1
was generated by: iGEM to SBOL conversion
description: VirA receptor (expressed)
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: ''A. tumefaciens'' C58, partsregistry
owned by: james
owned by: myers
mutable description: This BioBrick contains a ''virA'' receptor from ''Agrobacterium tumefaciens'' C58 under the control of a constitutive promotor. We used it to mutate ''virA'' by error-prone PCR. For screening the mutants BBa_K389011 is also needed.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The ''virA'' gene is under the control a medium strong constitutive promotor.
star: false
creator: Jonas Aretz
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_J23110
component: BBa_K389001
component: BBa_B0034
sequence annotation: BBa_J23110
sequence annotation: BBa_B0034
sequence annotation: BBa_K389001
sequence: https://synbiohub.org/public/igem/BBa_K389010_sequence/1

BBa_J23110
type: Component
persistent identity: component2219527
display id: component2219527
version: 1
top level: virA
definition: BBa_J23110
access: public

BBa_K389001
type: Component
persistent identity: component2219533
display id: component2219533
version: 1
top level: virA
definition: virA
access: public

BBa_B0034
type: Component
persistent identity: component2219529
display id: component2219529
version: 1
top level: virA
definition: BBa_B0034
access: public

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2219527
display id: annotation2219527
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219527/range2219527/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389010/annotation2219527/range2219527/1
type: Range
persistent identity: range2219527
display id: range2219527
version: 1
top level: virA
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2219529
display id: annotation2219529
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219529/range2219529/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389010/annotation2219529/range2219529/1
type: Range
persistent identity: range2219529
display id: range2219529
version: 1
top level: virA
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K389001
type: SequenceAnnotation
persistent identity: annotation2219533
display id: annotation2219533
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219533/range2219533/1
component: BBa_K389001

https://synbiohub.org/public/igem/BBa_K389010/annotation2219533/range2219533/1
type: Range
persistent identity: range2219533
display id: range2219533
version: 1
top level: virA
direction: direction/reverse
start: 62
end: 2566
orientation: inline

BBa_K389011
type: ComponentDefinition
persistent identity: BBa K389011
display id: BBa_K389011
version: 1
was generated by: iGEM to SBOL conversion
description: VirA screening device
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: synthetic ''virG'' gene, ''vir'' promotor from ''A. tumefaciens'' C58, partsregistry
owned by: james
owned by: myers
mutable description: This part contains: - a mutated ''virG'' response regulator from the virA/G receptor system from ''Agrobacterium tumefaciens'' which works in ''Escherichia coli'' without an ''rpoA'' gene from ''A. tumefaciens'' - a kanamycin resistance under the control of a ''vir'' promotor This device is used to screen ''virA'' receptor mutants. The ''virA'' receptor is mutated in the BBa_K389010 BioBrick. The better the receptor detects a substance, the more ''virG'' is phosphorylated and the stronger the kanamycin resistance from this BioBrick is expressed. The BioBricks BBa_K389010 and BBa_K389011 have to be used together in one cell.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: medium strong constitutive promotor, terminator to keep basal expression of kanamycin resistance low
star: false
creator: Jonas Aretz
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K389003
component: BBa_K389002
component: BBa_J23110
component: BBa_B0010
component: BBa_K389005
sequence annotation: BBa_J23110
sequence annotation: BBa_K389002
sequence annotation: BBa_B0010
sequence annotation: BBa_K389003
sequence annotation: BBa_K389005
sequence: https://synbiohub.org/public/igem/BBa_K389011_sequence/1

BBa_K389003
type: Component
persistent identity: component2078379
display id: component2078379
version: 1
top level: BBa_K389011
definition: Pvir
access: public

BBa_K389002
type: Component
persistent identity: component2078370
display id: component2078370
version: 1
top level: BBa_K389011
definition: virG
access: public

BBa_J23110
type: Component
persistent identity: component2078366
display id: component2078366
version: 1
top level: BBa_K389011
definition: BBa_J23110
access: public

BBa_B0010
type: Component
persistent identity: component2078373
display id: component2078373
version: 1
top level: BBa_K389011
definition: BBa_B0010
access: public

BBa_B0010
type: Component
persistent identity: component2078371
display id: component2078371
version: 1
top level: BBa_K389011
definition: BBa_B0010
access: public

BBa_K389005
type: Component
persistent identity: component2078382
display id: component2078382
version: 1
top level: BBa_K389011
definition: kanR
access: public

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2078366
display id: annotation2078366
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078366/range2078366/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389011/annotation2078366/range2078366/1
type: Range
persistent identity: range2078366
display id: range2078366
version: 1
top level: BBa_K389011
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2078370
display id: annotation2078370
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078370/range2078370/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389011/annotation2078370/range2078370/1
type: Range
persistent identity: range2078370
display id: range2078370
version: 1
top level: BBa_K389011
direction: direction/reverse
start: 44
end: 783
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2078371
display id: annotation2078371
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078371/range2078371/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K389011/annotation2078371/range2078371/1
type: Range
persistent identity: range2078371
display id: range2078371
version: 1
top level: BBa_K389011
direction: direction/forward
start: 792
end: 871
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2078373
display id: annotation2078373
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078373/range2078373/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K389011/annotation2078373/range2078373/1
type: Range
persistent identity: range2078373
display id: range2078373
version: 1
top level: BBa_K389011
direction: direction/forward
start: 880
end: 959
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2078379
display id: annotation2078379
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078379/range2078379/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389011/annotation2078379/range2078379/1
type: Range
persistent identity: range2078379
display id: range2078379
version: 1
top level: BBa_K389011
direction: direction/reverse
start: 968
end: 1125
orientation: inline

BBa_K389005
type: SequenceAnnotation
persistent identity: annotation2078382
display id: annotation2078382
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078382/range2078382/1
component: BBa_K389005

https://synbiohub.org/public/igem/BBa_K389011/annotation2078382/range2078382/1
type: Range
persistent identity: range2078382
display id: range2078382
version: 1
top level: BBa_K389011
direction: direction/reverse
start: 1132
end: 1950
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

virA
type: ComponentDefinition
persistent identity: BBa K389001
display id: BBa_K389001
version: 1
was generated by: iGEM to SBOL conversion
description: VirA receptor
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:18Z
mutable provenance: ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
mutable description: This is a gene for the ''virA'' receptor from ''Agrobacterium tumefaciens'' C58. An illegal ''PstI'' restriction site was removed by site-directed mutagenesis.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Illegal ''PstI'' restriction site removed
star: false
creator: Jonas Aretz
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: virA
sequence annotation: Illegal PstI site removed
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K389001_sequence/1

virA
type: SequenceAnnotation
persistent identity: annotation2077044
display id: annotation2077044
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389001/annotation2077044/range2077044/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K389001/annotation2077044/range2077044/1
type: Range
persistent identity: range2077044
display id: range2077044
version: 1
top level: virA
direction: direction/forward
start: 1
end: 2505
orientation: inline

Illegal PstI site removed
type: SequenceAnnotation
persistent identity: annotation2077043
display id: annotation2077043
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389001/annotation2077043/range2077043/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K389001/annotation2077043/range2077043/1
type: Range
persistent identity: range2077043
display id: range2077043
version: 1
top level: virA
direction: direction/forward
start: 187
end: 187
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078363
display id: annotation2078363
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389001/annotation2078363/range2078363/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K389001/annotation2078363/range2078363/1
type: Range
persistent identity: range2078363
display id: range2078363
version: 1
top level: virA
direction: direction/forward
start: 2503
end: 2505
orientation: inline

virG
type: ComponentDefinition
persistent identity: BBa K389002
display id: BBa_K389002
version: 1
was generated by: iGEM to SBOL conversion
description: Mutated virG
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:18Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: This is the ''virG'' response regulator from the virA/G receptor system. The virA/G receptor system is used by ''Agrobacterium tumefaciens'' to detect phenolic substances secreted by plants. In presence of these substances, ''virG'' is activated by the ''virA'' receptor and induces the transcription of genes under the control of a ''vir'' promotor. Normally, ''virG'' needs the ''rpoA'' RNA polymerase subunit from ''A. tumefaciens'' to work in ''Escherichia coli'' but this ''virG'' BioBrick is mutated, so works with the ''rpoA'' subunit from ''E. coli''.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: synthesized by Mr. Gene, so optimated codon usage, removal of illegal restriction sites
star: false
creator: Jonas Aretz
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: RBS
sequence annotation: virG
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K389002_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2078360
display id: annotation2078360
version: 1
top level: virG
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1
type: Range
persistent identity: range2078360
display id: range2078360
version: 1
top level: virG
direction: direction/forward
start: 4
end: 8
orientation: inline

virG
type: SequenceAnnotation
persistent identity: annotation2078361
display id: annotation2078361
version: 1
top level: virG
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1
type: Range
persistent identity: range2078361
display id: range2078361
version: 1
top level: virG
direction: direction/forward
start: 12
end: 737
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078362
display id: annotation2078362
version: 1
top level: virG
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1
type: Range
persistent identity: range2078362
display id: range2078362
version: 1
top level: virG
direction: direction/forward
start: 738
end: 740
orientation: inline

Pvir
type: ComponentDefinition
persistent identity: BBa K389003
display id: BBa_K389003
version: 1
was generated by: iGEM to SBOL conversion
description: virB promoter
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: ''A. tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
mutable description: This is a ''vir'' promotor from ''Agrobacterium tumefaciens'' C58. It is located on the TI-plasmid before the ''virB'' genes. It is activated by phosphorylated ''virG'' response regulator.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: contains already a RBS
star: false
creator: Jonas Aretz
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: vir-box
sequence annotation: -35
sequence annotation: -10
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K389003_sequence/1

vir-box
type: SequenceAnnotation
persistent identity: annotation2077848
display id: annotation2077848
version: 1
top level: Pvir
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077848/range2077848/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K389003/annotation2077848/range2077848/1
type: Range
persistent identity: range2077848
display id: range2077848
version: 1
top level: Pvir
direction: direction/forward
start: 33
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2077850
display id: annotation2077850
version: 1
top level: Pvir
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077850/range2077850/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K389003/annotation2077850/range2077850/1
type: Range
persistent identity: range2077850
display id: range2077850
version: 1
top level: Pvir
direction: direction/forward
start: 89
end: 93
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2077851
display id: annotation2077851
version: 1
top level: Pvir
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077851/range2077851/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K389003/annotation2077851/range2077851/1
type: Range
persistent identity: range2077851
display id: range2077851
version: 1
top level: Pvir
direction: direction/forward
start: 113
end: 117
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2077849
display id: annotation2077849
version: 1
top level: Pvir
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077849/range2077849/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K389003/annotation2077849/range2077849/1
type: Range
persistent identity: range2077849
display id: range2077849
version: 1
top level: Pvir
direction: direction/forward
start: 153
end: 157
orientation: inline

kanR
type: ComponentDefinition
persistent identity: BBa K389005
display id: BBa_K389005
version: 1
was generated by: iGEM to SBOL conversion
description: Kanamycin resistance
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: BioBrick BBa_P1003
owned by: james
owned by: myers
mutable description: This is a kanamycin resistance part without promotor. It is made from the BioBrick BBa_P1003 and is used for screenings.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: we removed the promotor from the kanamycin resistance gene in BioBrick BBa_P1003
star: false
creator: Jonas Aretz
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: kanamycin resistance
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K389005_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation2077058
display id: annotation2077058
version: 1
top level: kanR
location: https://synbiohub.org/public/igem/BBa_K389005/annotation2077058/range2077058/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K389005/annotation2077058/range2077058/1
type: Range
persistent identity: range2077058
display id: range2077058
version: 1
top level: kanR
direction: direction/forward
start: 1
end: 816
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078365
display id: annotation2078365
version: 1
top level: kanR
location: https://synbiohub.org/public/igem/BBa_K389005/annotation2078365/range2078365/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K389005/annotation2078365/range2078365/1
type: Range
persistent identity: range2078365
display id: range2078365
version: 1
top level: kanR
direction: direction/forward
start: 817
end: 819
orientation: inline

https://synbiohub.org/public/igem/BBa_K389014_sequence/1
type: Sequence
persistent identity: BBa K389014 sequence
display id: BBa_K389014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaatggaaggtattcaccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgccttgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacagtgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttgggagctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaactgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgcaagattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcgaacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcgacatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgattttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaagtgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggcctttgcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggtaatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgcttggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaagtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatgagcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctgctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccaggcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcgatattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttcaacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagacacattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactagaacgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaacatcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatgaatctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcgacagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcattgccgaaactgtgctcccccacattt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389010_sequence/1
type: Sequence
persistent identity: BBa K389010 sequence
display id: BBa_K389010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaatggaaggtattcaccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgccttgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacagtgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttgggagctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaactgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgcaagattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcgaacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcgacatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgattttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaagtgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggcctttgcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggtaatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgcttggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaagtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatgagcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctgctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccaggcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcgatattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttcaacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagacacattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactagaacgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaacatcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatgaatctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcgacagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcattgccgaaactgtgctcccccacattt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389011_sequence/1
type: Sequence
persistent identity: BBa K389011 sequence
display id: BBa_K389011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctactagagaagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagaggcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggagatactagatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23110_sequence/1
type: Sequence
persistent identity: BBa J23110 sequence
display id: BBa_J23110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389001_sequence/1
type: Sequence
persistent identity: BBa K389001 sequence
display id: BBa_K389001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatggaaggtattcaccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgccttgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacagtgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttgggagctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaactgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgcaagattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcgaacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcgacatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgattttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaagtgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggcctttgcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggtaatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgcttggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaagtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatgagcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctgctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccaggcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcgatattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttcaacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagacacattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactagaacgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaacatcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatgaatctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcgacagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcattgccgaaactgtgctcccccacatttttgagcctttctttacaacacgctcttgcagcggcggcacgggtctaggtcttgctgccgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389002_sequence/1
type: Sequence
persistent identity: BBa K389002 sequence
display id: BBa_K389002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389003_sequence/1
type: Sequence
persistent identity: BBa K389003 sequence
display id: BBa_K389003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389005_sequence/1
type: Sequence
persistent identity: BBa K389005 sequence
display id: BBa_K389005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z