##gff-version 3
##sequence-region BBa_K389015 1 5361
BBa_K389015	.	engineered_region	1	2566	.	+	0	ID=BBa_K389010;Name=BBa_K389010
BBa_K389015	.	promoter	1	35	.	+	0	ID=BBa_J23110;Name=BBa_J23110;Parent=BBa_K389010
BBa_K389015	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K389010
BBa_K389015	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K389015	.	CDS	62	2566	.	+	0	ID=BBa_K389001;Name=BBa_K389001;Parent=BBa_K389010
BBa_K389015	.	CDS	62	2566	.	+	0	ID=annotation2077044;Name=virA;Parent=BBa_K389001
BBa_K389015	.	stop_codon	2564	2566	.	+	0	ID=annotation2078363;Name=Stop codon;Parent=BBa_K389001
BBa_K389015	.	sequence_alteration	248	248	.	+	0	ID=annotation2077043;Name=Illegal PstI site removed;Parent=BBa_K389001
BBa_K389015	.	engineered_region	2575	5361	.	+	0	ID=BBa_K389012;Name=BBa_K389012
BBa_K389015	.	promoter	3542	3699	.	+	0	ID=BBa_K389003;Name=BBa_K389003;Parent=BBa_K389012
BBa_K389015	.	ribosome_entry_site	3694	3698	.	+	0	ID=annotation2077849;Name=rbs;Parent=BBa_K389003
BBa_K389015	.	non_covalent_binding_site	3574	3585	.	+	0	ID=annotation2077848;Name=vir-box;Parent=BBa_K389003
BBa_K389015	.	promoter	3630	3634	.	+	0	ID=annotation2077850;Name=-35;Parent=BBa_K389003
BBa_K389015	.	promoter	3654	3658	.	+	0	ID=annotation2077851;Name=-10;Parent=BBa_K389003
BBa_K389015	.	engineered_region	3706	5361	.	+	0	ID=BBa_K389004;Name=BBa_K389004;Parent=BBa_K389012
BBa_K389015	.	CDS	3706	5358	.	+	0	ID=annotation2077057;Name=Luciferase;Parent=BBa_K389004
BBa_K389015	.	stop_codon	5359	5361	.	+	0	ID=annotation2078364;Name=Stop codon;Parent=BBa_K389004
BBa_K389015	.	terminator	3454	3533	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K389012
BBa_K389015	.	stem_loop	3465	3508	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K389015	.	engineered_region	3454	3533	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K389015	.	terminator	3366	3445	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K389012
BBa_K389015	.	stem_loop	3377	3420	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K389015	.	engineered_region	3366	3445	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K389015	.	CDS	2618	3357	.	+	0	ID=BBa_K389002;Name=BBa_K389002;Parent=BBa_K389012
BBa_K389015	.	CDS	2629	3354	.	+	0	ID=annotation2078361;Name=virG;Parent=BBa_K389002
BBa_K389015	.	ribosome_entry_site	2621	2625	.	+	0	ID=annotation2078360;Name=RBS;Parent=BBa_K389002
BBa_K389015	.	stop_codon	3355	3357	.	+	0	ID=annotation2078362;Name=Stop codon;Parent=BBa_K389002
BBa_K389015	.	promoter	2575	2609	.	+	0	ID=BBa_J23110;Name=BBa_J23110;Parent=BBa_K389012
>BBa_K389015
tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaatggaaggtattca
ccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgcct
tgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacag
tgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttggga
gctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaac
tgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgca
agattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcg
aacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcg
acatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgat
tttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaag
tgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggccttt
gcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggt
aatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgctt
ggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaa
gtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatga
gcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctg
ctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccag
gcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcga
tattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttc
aacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagac
acattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactaga
acgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaac
atcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatga
atctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcg
acagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcatt
gccgaaactgtgctcccccacatttttgagcctttctttacaacacgctcttgcagcggcggcacgggtctaggtcttg
ctgccgtgcacggtcatgtcagcgcgcttgcaggatatattgacgttacttcagccgtagggcgagggacgcgcttcga
catttatctacctccttcctcgaagaagcccgtcagcccggacgcgttttttgggccctgtaaaacaccgcgtggcaac
ggagaaattgtggcattgattgagccagatcctgtcttgcgggaggtgtacgaagacaagatcgccgctctgggctatg
agccggtgggctttaagacatgtgcagacctttgcaattggatatcaaaaggcaagcaagccgatctggttctagttga
ccaatcgtctcttcccgagaatcagagtgctactgctttgcacgcagccttcaagacggcgtccatcatcattggagga
agtgatcttaaaatgtcactttccagcgatgacatgacgtccgcgctttttctgcccaaacctatatcctccaggacga
tggcctacgcgattcgtaccaagatcaaagcctaataatactagagtttacggctagctcagtcctaggtacaatgcta
gctactagagaagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagt
atctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgt
cgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgat
atccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgatt
ttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcg
ttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggc
ggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtg
aacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcg
tcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgat
gtccaagtgtcacatggtggcacgatggctgcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagaggcacccttaagtgc
atggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatc
gtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggagatactagatggaaga
tgccaaaaacattaagaagggcccagcgccattctacccactcgaagacgggaccgccggcgagcagctgcacaaagcc
atgaagcgctacgccctggtgcccggcaccatcgcctttaccgacgcacatatcgaggtggacattacctacgccgagt
acttcgagatgagcgttcggctggcagaagctatgaagcgctatgggctgaatacaaaccatcggatcgtggtgtgcag
cgagaatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcggtgtggctgtggccccagctaacgacatc
tacaacgagcgcgagctgctgaacagcatgggcatcagccagcccaccgtcgtattcgtgagcaagaaagggctgcaaa
agatcctcaacgtgcaaaagaagctaccgatcatacaaaagatcatcatcatggatagcaagaccgactaccagggctt
ccaaagcatgtacaccttcgtgacttcccatttgccacccggcttcaacgagtacgacttcgtgcccgagagcttcgac
cgggacaaaaccatcgccctgatcatgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccgca
ccgcttgtgtccgattcagtcatgcccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagcgt
ggtgccatttcaccacggcttcggcatgttcaccacgctgggctacttgatctgcggctttcgggtcgtgctcatgtac
cgcttcgaggaggagctattcttgcgcagcttgcaagactataagattcaatctgccctgctggtgcccacactattta
gcttcttcgctaagagcactctcatcgacaagtacgacctaagcaacttgcacgagatcgccagcggcggggcgccgct
cagcaaggaggtaggtgaggccgtggccaaacgcttccacctaccaggcatccgccagggctacggcctgacagaaaca
accagcgccattctgatcacccccgaaggggacgacaagcctggcgcagtaggcaaggtggtgcccttcttcgaggcta
aggtggtggacttggacaccggtaagacactgggtgtgaaccagcgcggcgagctgtgcgtccgtggccccatgatcat
gagcggctacgttaacaaccccgaggctacaaacgctctcatcgacaaggacggctggctgcacagcggcgacatcgcc
tactgggacgaggacgagcacttcttcatcgtggaccggctgaagagcctgatcaaatacaagggctaccaggtagccc
cagccgaactggagagcatcctgctgcaacaccccaacatcttcgacgccggggtcgccggcctgcccgacgacgatgc
cggcgagctgcccgccgcagtcgtcgtgctggaacacggtaaaaccatgaccgagaaggagatcgtggactatgtggcc
agccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggtgcctaaaggactgaccggcaagt
tggacgcccgcaagatccgcgagattctcattaaggccaagaagggcggcaagatcgccgtgtaataa