BBa_K389016
type: ComponentDefinition
persistent identity: BBa K389016
display id: BBa_K389016
version: 1
was generated by: iGEM to SBOL conversion
description: VirA/G reporter device mRFP
created: 2010-09-27T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: ''A. tumefaciens'' C58 TI-plasmid, partsregistry, Mr. Gene
owned by: james
owned by: myers
mutable description: This BioBrick is for testing the virA/G signaling system. The read-out is the mRFP <partinfo>E1010</partinfo>. This system contains an unmutated ''virA'', so it is possible to measure, how the natural virA/G system works and reacts.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: this is only for measuring the natural virA/G system
star: false
creator: Jonas Aretz
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K389013
component: BBa_K389010
sequence annotation: BBa_K389010
sequence annotation: BBa_K389013
sequence: https://synbiohub.org/public/igem/BBa_K389016_sequence/1

BBa_K389013
type: Component
persistent identity: component2277667
display id: component2277667
version: 1
top level: BBa_K389016
definition: BBa_K389013
access: public

BBa_K389010
type: Component
persistent identity: component2277648
display id: component2277648
version: 1
top level: BBa_K389016
definition: virA
access: public

BBa_K389010
type: SequenceAnnotation
persistent identity: annotation2277648
display id: annotation2277648
version: 1
top level: BBa_K389016
location: https://synbiohub.org/public/igem/BBa_K389016/annotation2277648/range2277648/1
component: BBa_K389010

https://synbiohub.org/public/igem/BBa_K389016/annotation2277648/range2277648/1
type: Range
persistent identity: range2277648
display id: range2277648
version: 1
top level: BBa_K389016
direction: direction/reverse
start: 1
end: 2566
orientation: inline

BBa_K389013
type: SequenceAnnotation
persistent identity: annotation2277667
display id: annotation2277667
version: 1
top level: BBa_K389016
location: https://synbiohub.org/public/igem/BBa_K389016/annotation2277667/range2277667/1
component: BBa_K389013

https://synbiohub.org/public/igem/BBa_K389016/annotation2277667/range2277667/1
type: Range
persistent identity: range2277667
display id: range2277667
version: 1
top level: BBa_K389016
direction: direction/reverse
start: 2575
end: 4411
orientation: inline

BBa_K389013
type: ComponentDefinition
persistent identity: BBa K389013
display id: BBa_K389013
version: 1
was generated by: iGEM to SBOL conversion
description: VirA reporter system mRFP
created: 2010-09-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: partsregistry, synthetic gene by Mr. Gene, TI-plasmid from ''Agrobacterium tumefaciens'' C58
owned by: james
owned by: myers
mutable description: With this system it is possible to measure the activity of a virA receptor and the vir promoter, respectively. The reporter is the mRFP from the partsregistry.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: medium strong constitutive promotor, terminator to keep basal expression of mRFP low
star: false
creator: Jonas Aretz
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J23110
component: BBa_B0017
component: BBa_K389002
component: BBa_E1010
component: BBa_K389003
sequence annotation: BBa_J23110
sequence annotation: BBa_K389002
sequence annotation: BBa_B0017
sequence annotation: BBa_K389003
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K389013_sequence/1

BBa_J23110
type: Component
persistent identity: component2263579
display id: component2263579
version: 1
top level: BBa_K389013
definition: BBa_J23110
access: public

BBa_B0017
type: Component
persistent identity: component2263588
display id: component2263588
version: 1
top level: BBa_K389013
definition: BBa_B0017
access: public

BBa_K389002
type: Component
persistent identity: component2263583
display id: component2263583
version: 1
top level: BBa_K389013
definition: virG
access: public

BBa_E1010
type: Component
persistent identity: component2263596
display id: component2263596
version: 1
top level: BBa_K389013
definition: mRFP1
access: public

BBa_K389003
type: Component
persistent identity: component2263593
display id: component2263593
version: 1
top level: BBa_K389013
definition: Pvir
access: public

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2263579
display id: annotation2263579
version: 1
top level: BBa_K389013
location: https://synbiohub.org/public/igem/BBa_K389013/annotation2263579/range2263579/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389013/annotation2263579/range2263579/1
type: Range
persistent identity: range2263579
display id: range2263579
version: 1
top level: BBa_K389013
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2263583
display id: annotation2263583
version: 1
top level: BBa_K389013
location: https://synbiohub.org/public/igem/BBa_K389013/annotation2263583/range2263583/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389013/annotation2263583/range2263583/1
type: Range
persistent identity: range2263583
display id: range2263583
version: 1
top level: BBa_K389013
direction: direction/reverse
start: 44
end: 783
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2263588
display id: annotation2263588
version: 1
top level: BBa_K389013
location: https://synbiohub.org/public/igem/BBa_K389013/annotation2263588/range2263588/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389013/annotation2263588/range2263588/1
type: Range
persistent identity: range2263588
display id: range2263588
version: 1
top level: BBa_K389013
direction: direction/reverse
start: 792
end: 959
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2263593
display id: annotation2263593
version: 1
top level: BBa_K389013
location: https://synbiohub.org/public/igem/BBa_K389013/annotation2263593/range2263593/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389013/annotation2263593/range2263593/1
type: Range
persistent identity: range2263593
display id: range2263593
version: 1
top level: BBa_K389013
direction: direction/reverse
start: 968
end: 1125
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2263596
display id: annotation2263596
version: 1
top level: BBa_K389013
location: https://synbiohub.org/public/igem/BBa_K389013/annotation2263596/range2263596/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K389013/annotation2263596/range2263596/1
type: Range
persistent identity: range2263596
display id: range2263596
version: 1
top level: BBa_K389013
direction: direction/reverse
start: 1132
end: 1837
orientation: inline

virA
type: ComponentDefinition
persistent identity: BBa K389010
display id: BBa_K389010
version: 1
was generated by: iGEM to SBOL conversion
description: VirA receptor (expressed)
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: ''A. tumefaciens'' C58, partsregistry
owned by: james
owned by: myers
mutable description: This BioBrick contains a ''virA'' receptor from ''Agrobacterium tumefaciens'' C58 under the control of a constitutive promotor. We used it to mutate ''virA'' by error-prone PCR. For screening the mutants BBa_K389011 is also needed.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The ''virA'' gene is under the control a medium strong constitutive promotor.
star: false
creator: Jonas Aretz
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_J23110
component: BBa_K389001
component: BBa_B0034
sequence annotation: BBa_J23110
sequence annotation: BBa_B0034
sequence annotation: BBa_K389001
sequence: https://synbiohub.org/public/igem/BBa_K389010_sequence/1

BBa_J23110
type: Component
persistent identity: component2219527
display id: component2219527
version: 1
top level: virA
definition: BBa_J23110
access: public

BBa_K389001
type: Component
persistent identity: component2219533
display id: component2219533
version: 1
top level: virA
definition: virA
access: public

BBa_B0034
type: Component
persistent identity: component2219529
display id: component2219529
version: 1
top level: virA
definition: BBa_B0034
access: public

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2219527
display id: annotation2219527
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219527/range2219527/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389010/annotation2219527/range2219527/1
type: Range
persistent identity: range2219527
display id: range2219527
version: 1
top level: virA
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2219529
display id: annotation2219529
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219529/range2219529/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389010/annotation2219529/range2219529/1
type: Range
persistent identity: range2219529
display id: range2219529
version: 1
top level: virA
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K389001
type: SequenceAnnotation
persistent identity: annotation2219533
display id: annotation2219533
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219533/range2219533/1
component: BBa_K389001

https://synbiohub.org/public/igem/BBa_K389010/annotation2219533/range2219533/1
type: Range
persistent identity: range2219533
display id: range2219533
version: 1
top level: virA
direction: direction/reverse
start: 62
end: 2566
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

BBa_B0017
type: ComponentDefinition
persistent identity: BBa B0017
display id: BBa_B0017
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0010)
created: 2004-01-08T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator with two copies of <bb_part>BBa_B0010</bb_part>
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Austin Che
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0010
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0017_sequence/1

BBa_B0010
type: Component
persistent identity: component939331
display id: component939331
version: 1
top level: BBa_B0017
definition: BBa_B0010
access: public

BBa_B0010
type: Component
persistent identity: component939337
display id: component939337
version: 1
top level: BBa_B0017
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation939331
display id: annotation939331
version: 1
top level: BBa_B0017
location: https://synbiohub.org/public/igem/BBa_B0017/annotation939331/range939331/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0017/annotation939331/range939331/1
type: Range
persistent identity: range939331
display id: range939331
version: 1
top level: BBa_B0017
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation939337
display id: annotation939337
version: 1
top level: BBa_B0017
location: https://synbiohub.org/public/igem/BBa_B0017/annotation939337/range939337/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0017/annotation939337/range939337/1
type: Range
persistent identity: range939337
display id: range939337
version: 1
top level: BBa_B0017
direction: direction/forward
start: 89
end: 168
orientation: inline

virA
type: ComponentDefinition
persistent identity: BBa K389001
display id: BBa_K389001
version: 1
was generated by: iGEM to SBOL conversion
description: VirA receptor
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:18Z
mutable provenance: ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
mutable description: This is a gene for the ''virA'' receptor from ''Agrobacterium tumefaciens'' C58. An illegal ''PstI'' restriction site was removed by site-directed mutagenesis.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Illegal ''PstI'' restriction site removed
star: false
creator: Jonas Aretz
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: virA
sequence annotation: Illegal PstI site removed
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K389001_sequence/1

virA
type: SequenceAnnotation
persistent identity: annotation2077044
display id: annotation2077044
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389001/annotation2077044/range2077044/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K389001/annotation2077044/range2077044/1
type: Range
persistent identity: range2077044
display id: range2077044
version: 1
top level: virA
direction: direction/forward
start: 1
end: 2505
orientation: inline

Illegal PstI site removed
type: SequenceAnnotation
persistent identity: annotation2077043
display id: annotation2077043
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389001/annotation2077043/range2077043/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K389001/annotation2077043/range2077043/1
type: Range
persistent identity: range2077043
display id: range2077043
version: 1
top level: virA
direction: direction/forward
start: 187
end: 187
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078363
display id: annotation2078363
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389001/annotation2078363/range2078363/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K389001/annotation2078363/range2078363/1
type: Range
persistent identity: range2078363
display id: range2078363
version: 1
top level: virA
direction: direction/forward
start: 2503
end: 2505
orientation: inline

virG
type: ComponentDefinition
persistent identity: BBa K389002
display id: BBa_K389002
version: 1
was generated by: iGEM to SBOL conversion
description: Mutated virG
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:18Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: This is the ''virG'' response regulator from the virA/G receptor system. The virA/G receptor system is used by ''Agrobacterium tumefaciens'' to detect phenolic substances secreted by plants. In presence of these substances, ''virG'' is activated by the ''virA'' receptor and induces the transcription of genes under the control of a ''vir'' promotor. Normally, ''virG'' needs the ''rpoA'' RNA polymerase subunit from ''A. tumefaciens'' to work in ''Escherichia coli'' but this ''virG'' BioBrick is mutated, so works with the ''rpoA'' subunit from ''E. coli''.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: synthesized by Mr. Gene, so optimated codon usage, removal of illegal restriction sites
star: false
creator: Jonas Aretz
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: RBS
sequence annotation: virG
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K389002_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2078360
display id: annotation2078360
version: 1
top level: virG
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1
type: Range
persistent identity: range2078360
display id: range2078360
version: 1
top level: virG
direction: direction/forward
start: 4
end: 8
orientation: inline

virG
type: SequenceAnnotation
persistent identity: annotation2078361
display id: annotation2078361
version: 1
top level: virG
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1
type: Range
persistent identity: range2078361
display id: range2078361
version: 1
top level: virG
direction: direction/forward
start: 12
end: 737
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078362
display id: annotation2078362
version: 1
top level: virG
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1
type: Range
persistent identity: range2078362
display id: range2078362
version: 1
top level: virG
direction: direction/forward
start: 738
end: 740
orientation: inline

Pvir
type: ComponentDefinition
persistent identity: BBa K389003
display id: BBa_K389003
version: 1
was generated by: iGEM to SBOL conversion
description: virB promoter
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: ''A. tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
mutable description: This is a ''vir'' promotor from ''Agrobacterium tumefaciens'' C58. It is located on the TI-plasmid before the ''virB'' genes. It is activated by phosphorylated ''virG'' response regulator.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: contains already a RBS
star: false
creator: Jonas Aretz
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: vir-box
sequence annotation: -35
sequence annotation: -10
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K389003_sequence/1

vir-box
type: SequenceAnnotation
persistent identity: annotation2077848
display id: annotation2077848
version: 1
top level: Pvir
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077848/range2077848/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K389003/annotation2077848/range2077848/1
type: Range
persistent identity: range2077848
display id: range2077848
version: 1
top level: Pvir
direction: direction/forward
start: 33
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2077850
display id: annotation2077850
version: 1
top level: Pvir
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077850/range2077850/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K389003/annotation2077850/range2077850/1
type: Range
persistent identity: range2077850
display id: range2077850
version: 1
top level: Pvir
direction: direction/forward
start: 89
end: 93
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2077851
display id: annotation2077851
version: 1
top level: Pvir
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077851/range2077851/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K389003/annotation2077851/range2077851/1
type: Range
persistent identity: range2077851
display id: range2077851
version: 1
top level: Pvir
direction: direction/forward
start: 113
end: 117
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2077849
display id: annotation2077849
version: 1
top level: Pvir
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077849/range2077849/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K389003/annotation2077849/range2077849/1
type: Range
persistent identity: range2077849
display id: range2077849
version: 1
top level: Pvir
direction: direction/forward
start: 153
end: 157
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K389016_sequence/1
type: Sequence
persistent identity: BBa K389016 sequence
display id: BBa_K389016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaatggaaggtattcaccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgccttgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacagtgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttgggagctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaactgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgcaagattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcgaacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcgacatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgattttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaagtgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggcctttgcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggtaatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgcttggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaagtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatgagcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctgctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccaggcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcgatattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttcaacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagacacattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactagaacgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaacatcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatgaatctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcgacagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcattgccgaaactgtgctcccccacattt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389013_sequence/1
type: Sequence
persistent identity: BBa K389013 sequence
display id: BBa_K389013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctactagagaagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagaggcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggagatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389010_sequence/1
type: Sequence
persistent identity: BBa K389010 sequence
display id: BBa_K389010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaatggaaggtattcaccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgccttgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacagtgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttgggagctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaactgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgcaagattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcgaacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcgacatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgattttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaagtgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggcctttgcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggtaatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgcttggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaagtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatgagcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctgctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccaggcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcgatattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttcaacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagacacattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactagaacgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaacatcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatgaatctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcgacagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcattgccgaaactgtgctcccccacattt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23110_sequence/1
type: Sequence
persistent identity: BBa J23110 sequence
display id: BBa_J23110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0017_sequence/1
type: Sequence
persistent identity: BBa B0017 sequence
display id: BBa_B0017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389001_sequence/1
type: Sequence
persistent identity: BBa K389001 sequence
display id: BBa_K389001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatggaaggtattcaccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgccttgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacagtgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttgggagctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaactgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgcaagattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcgaacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcgacatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgattttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaagtgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggcctttgcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggtaatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgcttggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaagtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatgagcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctgctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccaggcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcgatattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttcaacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagacacattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactagaacgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaacatcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatgaatctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcgacagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcattgccgaaactgtgctcccccacatttttgagcctttctttacaacacgctcttgcagcggcggcacgggtctaggtcttgctgccgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389002_sequence/1
type: Sequence
persistent identity: BBa K389002 sequence
display id: BBa_K389002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389003_sequence/1
type: Sequence
persistent identity: BBa K389003 sequence
display id: BBa_K389003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z